Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (11): 2628-2642.DOI: 10.1016/S2095-3119(19)62846-7

所属专题: 麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources

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  • 收稿日期:2019-07-30 出版日期:2020-11-01 发布日期:2020-10-15

Grain proteomic analysis reveals central stress responsive proteins caused by wheat-Haynaldia villosa 6VS/6AL translocation

ZOU Rong1*, WU Ji-su1*, WANG Ruo-mei1, YAN Yue-ming1, 2  
  

  1. 1 College of Life Science, Capital Normal University, Beijing 100048, P.R.China
    2 Hubei Collaborative Innovation Center for Grain Industry (HCICGI), Yangtze University, Jingzhou 434023, P.R.China
  • Received:2019-07-30 Online:2020-11-01 Published:2020-10-15
  • Contact: Correspondence YAN Yue-ming, Tel/Fax: +86-10-68902777, E-mail: yanym@cnu.edu.cn
  • About author:* These authors contributed equally to this study.
  • Supported by:
    This research was financially supported by the National Key R&D Program of China (2016YFD0100502) and the National Natural Science Foundation of China (31771773).

Abstract: Haynaldia villosa (2n=14, VV), a wild grass of the subtribe Triticeae, serves as potential gene resources for wheat genetic improvement.  In this study, the proteome characterization during grain development of Yangmai 5 and Yangmai 5-H. villosa 6VS/6AL translocation line was investigated by a comparative proteomic approach.  Two-dimensional electrophoresis identified 81 differentially accumulated proteins (DAPs) during five grain developmental stages in wheat-H. villosa translocation line.  These proteins were mainly involved in stress defense, storage protein, energy metabolism, protein metabolism and folding, carbon metabolism, nitrogen metabolism, and starch metabolism.  In particular, 6VS/6AL translocation led to significant upregulation of 36 DAPs and specific expression of 11 DAPs such as chitinase, thaumatin-like proteins, glutathione transferase, α-amylase inhibitor, heat shock proteins, and betaine aldehyde dehydrogenase.  These proteins mainly involved in biotic and abiotic stress responses.  Further analysis found that the upstream 1 500 promoter regions of these stress-responsive DAP genes contained multiple high-frequency cis-acting elements related to stress defense such as abscisic acid response element ABRE, methyl jasmonate (MeJA)-response element TGACG-motif and CGTCA-motif involved in oxidative stress and antioxidant response element (ARE).  RNA-seq and RT-qPCR analyses revealed the high expression of these stress-defensive DAP genes in the developing grains, particularly at the early-middle grain filling stages.  Our results demonstrated that 6VS chromosome of H. villosa contains abundant stress-defensive proteins that have potential values for wheat genetic improvement.

Key words: 6VS/6AL translocation ,  grain development ,  proteome ,  DAPs , cis-acting elements ,  adverse defense