The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis

doi:10.1016/S2095-3119(20)63160-4

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (11): 2616-2627.DOI: 10.1016/S2095-3119(20)63160-4

所属专题：麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources

收稿日期:2019-07-18 出版日期:2020-11-01 发布日期:2020-10-15

The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis

WU Tian-ci^{1, 2}, ZHU Xiu-liang², LÜ Liang-jie³, CHEN Xi-yong³, XU Gang-biao¹, ZHANG Zeng-yan²

¹ Laboratory of Forestry Genetics, Central South University of Forestry and Technology, Changsha 410004, P.R.China
² National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
³ Institute for Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.China

Received:2019-07-18 Online:2020-11-01 Published:2020-10-15
Contact: Correspondence ZHANG Zeng-yan, Tel: +86-10-82108781, E-mail: zhangzengyan@caas.cn; XU Gang-biao, E-mail: gangbiaoxu@163.com
Supported by:
This study was funded by the Natural Science Foundation of China (31771789), the National Key Project for Research on Transgenic Biology, China (2016ZX08002-001-004), and Young Elite Scientists Sponsorship Program by China Association for Science and Technology (2018QNRC001).

摘要/Abstract

Abstract:

Receptor-like cytoplasmic kinases (RLCKs) represent a large family of proteins in plants. In Arabidopsis and rice, several RLCKs in subfamily VII (RLCKs-VII) have been implicated in pathogen-associated molecular pattern-triggered immunity and basal resistance against bacterial and fungal pathogens. However, little is known about roles of RLCKs-VII of the important crop common wheat (Triticum aestivum) in immune responses. Here, we isolated a RLCK-VII-encoding gene from wheat, designated as TaRLCK1B, and investigated its role in host immune response to infection of a necrotrophic fungus Rhizoctonia cerealis that is a major pathogen of sharp eyespot, a destructive disease of wheat. RNA-sequencing and RT-qPCR analyses showed that transcriptional level of TaRLCK1B was significantly higher in sharp eyespot-resistant wheat cultivars than in susceptible wheat cultivars. The gene transcription was rapidly and markedly elevated in the resistant wheat cultivars by R. cerealis infection. The TaRLCK1B protein was closely related to OsRLCK176, a rice resistance-related RLCKs-VII, with 84.03% identity. Virus-induced gene silencing plus wheat response to R. cerealis assay results indicated that silencing of TaRLCK1 impaired resistance to R. cerealis. Meantime, silencing of TaRLCK1 significantly elevated both the content of H₂O₂ (a major kind of reactive oxygen species, ROS) and the transcriptional level of the ROS-generating enzyme-encoding gene RBOH, but repressed the expression of the ROS-scavenging enzyme-encoding gene CAT1 at 18 hours after inoculation (hai) with R. cerealis. Taken together, these data suggested that TaRLCK1B was required for the early immune response of wheat to R. cerealis through modulating ROS signaling in wheat.

Key words: common wheat (Triticum aestivum) , H₂O₂ , immune respons , , reactive oxygen species , receptor-like cytoplasmic kinase , Rhizoctonia cerealis

. [J]. Journal of Integrative Agriculture, 2020, 19(11): 2616-2627.

WU Tian-ci, ZHU Xiu-liang, LÜ Liang-jie, CHEN Xi-yong, XU Gang-biao, ZHANG Zeng-yan. The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis[J]. Journal of Integrative Agriculture, 2020, 19(11): 2616-2627.

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The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis

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