Genome-wide association analysis for stripe rust resistance in spring wheat (Triticum aestivum L.) germplasm

doi:10.1016/S2095-3119(19)62841-8

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (8): 2035-2043.DOI: 10.1016/S2095-3119(19)62841-8

所属专题：植物抗病遗传合辑Plant Disease-resistance Genetics

收稿日期:2019-08-08 出版日期:2020-08-01 发布日期:2020-06-21

Genome-wide association analysis for stripe rust resistance in spring wheat (Triticum aestivum L.) germplasm

Sher MUHAMMAD^1*, Muhammad SAJJAD^2*, Sultan Habibullah KHAN³, Muhammad SHAHID¹, Muhammad ZUBAIR¹, Faisal Saeed AWAN³, Azeem Iqbal KHAN⁴, Muhammad Salman MUBARAK³, Ayesha TAHIR², Muhammad UMER², Rumana KEYANI², Muhammad Inam AFZAL², Irfan MANZOOR¹, Javed Iqbal WATTOO⁵, Aziz-ur REHMAN⁶

¹ Department of Bioinformatics and Biotechnology, GC University, Faisalabad 38090, Pakistan
² Department of Biosciences, COMSATS University Islamabad (CUI), Islamabad 45550, Pakistan
³ Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38000, Pakistan
⁴ Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Pakistan
⁵ Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore 54000, Pakistan
⁶ Wheat Research Institute, Ayub Agricultural Research Institute (AARI), Faisalabad 38950, Pakistan

Received:2019-08-08 Online:2020-08-01 Published:2020-06-21
Contact: Correspondence Muhammad Sajjad, Tel: +92-334-6609080, E-mail: muhammad.sajjad@comsats.edu.pk; Sultan Habibullah KHAN, Tel: +92-333-9917733, E-mail: sultan@uaf.edu.pk
About author:* These authors contributed equally to this study.
Supported by:
The authors acknowledge Higher Education Commission (HEC), Islamabad, Pakistan for providing for funding (21-179/SRGP/R&D/HEC/2014) and the International Research Support Initiative Program (IRSIP) Scholarship for this research work.

摘要/Abstract

Abstract:

Stripe rust is a continuous threat to wheat crop all over the world. It causes considerable yield losses in wheat crop every year. Continuous deployment of adult plant resistance (APR) genes in newly developing wheat cultivars is the most judicious strategy to combat this disease. Herein, we dissected the genetics underpinning stripe rust resistance in Pakistani wheat germplasm. An association panel of 94 spring wheat genotypes was phenotyped for two years to score the infestation of stripe rust on each accession and was scanned with 203 polymorphic SSRs. Based on D´ measure, linkage disequilibrium (LD) exhibited between loci distant up to 45 cM. Marker-trait associations (MTAs) were determined using mixed linear model (MLM). Total 31 quantitative trait loci (QTLs) were observed on all 21 wheat chromosomes. Twelve QTLs were newly discovered as well as 19 QTLs and 35 previously reported Yr genes were validated in Pakistani wheat germplasm. The major QTLs were QYr.uaf.2AL and QYr.uaf.3BS (PVE, 11.9%). Dissection of genes from the newly observed QTLs can provide new APR genes to improve genetic resources for APR resistance in wheat crop.

Key words: wheat , Puccinia striiformis , LD , GWAS , MTA , PCoA

. [J]. Journal of Integrative Agriculture, 2020, 19(8): 2035-2043.

Sher MUHAMMAD, Muhammad SAJJAD, Sultan Habibullah KHAN, Muhammad SHAHID, Muhammad ZUBAIR, Faisal Saeed AWAN, Azeem Iqbal KHAN, Muhammad Salman MUBARAK, Ayesha TAHIR, Muhammad Umer, Rumana KEYANI, Muhammad Inam AFZAL, Irfan MANZOOR, Javed Iqbal WATTOO, Aziz-ur REHMAN. Genome-wide association analysis for stripe rust resistance in spring wheat (Triticum aestivum L.) germplasm[J]. Journal of Integrative Agriculture, 2020, 19(8): 2035-2043.

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Genome-wide association analysis for stripe rust resistance in spring wheat (Triticum aestivum L.) germplasm

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