Expression and contribution of microphthalmia-associated transcription factor to the melanin deposition in Liancheng white ducks

doi:10.1016/S2095-3119(19)62736-X

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (3): 800-809.DOI: 10.1016/S2095-3119(19)62736-X

收稿日期:2018-08-13 出版日期:2020-03-01 发布日期:2020-03-04

Expression and contribution of microphthalmia-associated transcription factor to the melanin deposition in Liancheng white ducks

XIN Qing-wu¹, MIAO Zhong-wei¹, LIU Zhao-yuan², LI Li¹, ZHANG Lin-li¹, ZHU Zhi-ming¹, ZHANG Zheng-hong², ZHENG Nen-zhu¹, WANG Zheng-chao²

¹ Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.China
² Provincial Key Laboratory for Developmental Biology and Neurosciences/Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou 350007, P.R.China

Received:2018-08-13 Online:2020-03-01 Published:2020-03-04
Contact: Correspondence XIN Qing-wu, E-mail: xinqingwu6656@126.com; ZHENG Nen-zhu, E-mail: zhengnz@163.com; WANG Zheng-chao, E-mail: zcwang@fjnu.edu.cn
Supported by:
This study was supported by the National Natural Science Foundation of China (31372474), the National Guidance Project for Local Science and Technology Development, China (2018L3004), the National and FNU Training Program of Innovation and Enterpreneurship for Undergraduates, China (201910394023, CXXL2019268 and CXXL2019274), the Fujian Province Public Welfare Scientific Project, China (018R1023-2), the Fujian Development and Reform Commission “Five News” Project of China (fjfgw201806) and the Young Talent Innovation Funding of Fujian Academy of Agricultural Sciences of China (YC2017-7 and MYQJ2015-5).

摘要/Abstract

Abstract:

The present study investigates the expression of microphthalmia-associated transcription factor (MITF) and its contribution to the melanin deposition in Liancheng white ducks. Nested PCR was used to clone the MITF gene sequence from the skin tissue of female Liancheng white ducks. Ultraviolet spectrophotometry was used to detect the melanin deposition. MITF mRNA expression and melanin deposition in different tissues and organs were detected and their correlation was analyzed. The MITF gene (GenBank number: MG516570) was 1 323 bp in length, contains a complete CDS region (34–1 323 bp) and codes 429 amino acids with 100% homology to the MITF of Anas platyrhynchos and over 95% homology to those of Gallus gallus and Coturnix japonica. Genetic evolution analysis reveals a close relationship of Liancheng white ducks with A. platyrhynchos, and also to lesser extents with Anser cygnoides, silky fowl and G. gallus, as well as Sus scrofa, Ovis aries and other mammals. Real-time quantitative PCR (qPCR) analysis demonstrated that MITF was expressed in skin, gizzard, liver, kidney and muscle, and of these tissues, its expression was the highest in the skin tissue (skin>gizzard>liver>kidney>muscle). Ultraviolet spectrophotometry showed that melanin deposition was positively correlated with the MITF expression level in these five tissues and organs (P<0.05). Together, these results demonstrated a tissue-specific pattern of MITF expression and a positive correlation between MITF expression and melanin deposition, indicating that MITF expression may contribute to the melanin deposition in Liancheng white ducks.

Key words: microphthalmia-associated transcription factor , genetic evolution analysis , melanin deposition , Liancheng white ducks

. [J]. Journal of Integrative Agriculture, 2020, 19(3): 800-809.

XIN Qing-wu, MIAO Zhong-wei, LIU Zhao-yuan, LI Li, ZHANG Lin-li, ZHU Zhi-ming, ZHANG Zheng-hong, ZHENG Nen-zhu, WANG Zheng-chao . Expression and contribution of microphthalmia-associated transcription factor to the melanin deposition in Liancheng white ducks[J]. Journal of Integrative Agriculture, 2020, 19(3): 800-809.

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Expression and contribution of microphthalmia-associated transcription factor to the melanin deposition in Liancheng white ducks

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