Genetic parameter estimation and genome-wide association study (GWAS) of red blood cell count at three stages in a Duroc×Erhualian pig population

doi:10.1016/S2095-3119(19)62773-5

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (3): 793-799.DOI: 10.1016/S2095-3119(19)62773-5

收稿日期:2018-10-19 出版日期:2020-03-01 发布日期:2020-03-04

Genetic parameter estimation and genome-wide association study (GWAS) of red blood cell count at three stages in a Duroc×Erhualian pig population

NAN Jiu-hong^1*, YIN Li-lin^1*, TANG Zhen-shuang¹, CHEN Jian-hai¹, ZHANG Jie¹, WANG Hai-yan^{1, 2}, DU Xiao-yong^{1, 2}, LIU Xiang-dong^{1, 3}

¹ Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, P.R.China
² Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan 430070, P.R.China
³ Key Lab of Swine Healthy Breeding of Ministry of Agriculture and Rural Affairs, Guangxi Yangxiang Co., Ltd., Guigang 537100, P.R.China

Received:2018-10-19 Online:2020-03-01 Published:2020-03-04
Contact: Correspondence LIU Xiang-dong, E-mail: liuxiangdong@mail.hzau.edu.cn; DU Xiao-yong, E-mail: duxiaoyong@mail.hzau.edu.cn
About author:* These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31572375, NSFC-CGIAR31361140365), the Fundamental Research Funds for the Central Universities of China (2662016PY006), the National High Technology Research and Development Program of China (2013AA102502), the earmarked fund for China Agriculture Research System (CARS-35), and the Dabeinong Group Promoted Project for Young Scholar of Huazhong Agricultural University, China (2017DBN019).

摘要/Abstract

Abstract:

Red blood cells play an essential role in the immune system. Moreover, red blood cell count (RBC) is an important clinical indicator of various diseases, including anemia, type 2 diabetes and the metabolic syndrome. Thus, it is necessary to reveal the genetic mechanism of RBC for animal disease resistance breeding. However, quite a few studies had focused on porcine RBC, especially at different stages. Thus, studies on porcine RBC at different stages are needed for disease resistant breeding. In this study, the porcine RBC of 20-, 33-, and 80-day old were measured, and genetic parameter estimation and genome-wide association study (GWAS) were both performed. As a result, the heritability was about 0.6 at the early stages, much higher than that at 80 days. Nine novel genome wide significant single nucleotide polymorphisms (SNPs), located at Sus scrofa chromosome (SSC)3, 4, 8, 9, 10 and 15, respectively, were identified. Further, TGFβ2, TMCC2 and PPP1R15B genes were identified as important candidate genes of porcine red blood cell count. So different SNPs and candidate genes were found significantly associated with porcine RBC at different stages, suggesting that different genes might play key roles on porcine RBC at different stages. Overall, new evidences were offered in this study for the genetic bases of animal RBC, and that the SNPs and candidate genes would be useful for disease resistant breeding of pig.

Key words: RBC , genome-wide association , SNP , candidate gene , pig

. [J]. Journal of Integrative Agriculture, 2020, 19(3): 793-799.

NAN Jiu-hong, YIN Li-lin, TANG Zhen-shuang, CHEN Jian-hai, ZHANG Jie, WANG Hai-yan, DU Xiao-yong, LIU Xiang-dong . Genetic parameter estimation and genome-wide association study (GWAS) of red blood cell count at three stages in a Duroc×Erhualian pig population[J]. Journal of Integrative Agriculture, 2020, 19(3): 793-799.

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Genetic parameter estimation and genome-wide association study (GWAS) of red blood cell count at three stages in a Duroc×Erhualian pig population

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