OsHemA gene, encoding glutamyl-tRNA reductase (GluTR) is essential for chlorophyll biosynthesis in rice (Oryza sativa)

doi:10.1016/S2095-3119(19)62710-3

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (3): 612-623.DOI: 10.1016/S2095-3119(19)62710-3

所属专题：水稻遗传育种合辑Rice Genetics · Breeding · Germplasm Resources

收稿日期:2018-11-29 出版日期:2020-03-01 发布日期:2020-03-04

OsHemA gene, encoding glutamyl-tRNA reductase (GluTR) is essential for chlorophyll biosynthesis in rice (Oryza sativa)

ZENG Zhao-qiong^{1, 2*}, LIN Tian-zi^1*, ZHAO Jie-yu¹, ZHENG Tian-hui¹, XU Le-feng¹, WANG Yi-hua¹, LIU Ling-long¹, JIANG Ling¹, CHEN Sai-hua³, WAN Jian-min¹

¹State Key Laboratory for Crop Genetics & Germplasm Enhancement/Jiangsu Provincial Center of Plant Gene Engineering/College of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.China
² Nanchong Academy of Agricultural Sciences, Nanchong 637000, P.R.China
³ Key Laboratory of Plant Functional Genomics, Ministry of Education/Co?Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China

Received:2018-11-29 Online:2020-03-01 Published:2020-03-04
Contact: Correspondence CHEN Sai-hua, Tel/Fax: +86-514-87972178, E-mail: chensaihua@yzu.edu.cn; WAN Jian-min, E-mail: wanjm@njau.edu.cn
About author:* These authors contributed equally to this study.
Supported by:
This research was supported by the National Key Research and Development Program of China (2016YFD0101801), the National Excellent Doctoral Dissertation of China (201262), the Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, China, the Collaborative Innovation Center for Hybrid Rice in Yangtze River, China, and the Jiangsu Collaborative Innovation Center for Modern Crop Production, China, the National High-Tech R&D Program of China (2014AA10A603-15), the National Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD01B02-16), and the Jiangsu Science and Technology Development Program, China (BE2014394 and BE2015363).

摘要/Abstract

Abstract:

Chlorophyll (Chl) biosynthesis is essential for photosynthesis and plant growth. Glutamyl-tRNA reductase (GluTR) catalyzes glutamyl-tRNA into glutamate-1-semialdehyde (GSA) and initiates the chlorophyll biosynthesis. Even though the main role of GluTR has been established, the effects caused by natural variations in its corresponding gene remain largely unknown. Here, we characterized a spontaneous mutant in paddy field with Chl biosynthesis deficiency, designated as cbd1. With intact thylakoid lamellar structure, the cbd1 plant showed light green leaves and reduced Chl and carotenoids (Cars) content significantly compared to the wild type. By map-based gene cloning, the mutation was restricted within a 57-kb region on chromosome 10, in which an mPingA miniature inverted-repeat transposable element (MITE) inserted in the promoter region of OsHemA gene. Both leaf color and the pigment contents in cbd1 were recovered in a complementation test, confirming OsHemA was responsible for the mutant phenotype. OsHemA was uniquely predicted to encode GluTR and its expression level was dramatically repressed in cbd1. Transient transformation in protoplasts demonstrated that GluTR localized in chloroplasts and a signal peptide exists in its N-terminus. A majority of Chl biosynthesis genes, except for POR and CHLG, were down-regulated synchronously by the repression of OsHemA, suggesting that an attenuation occurred in the Chl biosynthesis pathway. Interestingly, we found major agronomic traits involved in rice yield were statistically unaffected, except for the number of full grains per panicle was increased in cbd1. Collectively, OsHemA plays an essential role in Chl biosynthesis in rice and its weak allele can adjust leaf color and Chls content without compromise to rice yield.

Key words: OsHemA , GluTR , chlorophyll biosynthesis

. [J]. Journal of Integrative Agriculture, 2020, 19(3): 612-623.

ZENG Zhao-qiong, LIN Tian-zi, ZHAO Jie-yu, ZHENG Tian-hui, XU Le-feng, WANG Yi-hua, LIU Ling-long, JIANG Ling, CHEN Sai-hua, WAN Jian-min . OsHemA gene, encoding glutamyl-tRNA reductase (GluTR) is essential for chlorophyll biosynthesis in rice (Oryza sativa)[J]. Journal of Integrative Agriculture, 2020, 19(3): 612-623.

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OsHemA gene, encoding glutamyl-tRNA reductase (GluTR) is essential for chlorophyll biosynthesis in rice (Oryza sativa)

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