Functional characterization of (E)-β-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin

doi:10.1016/S2095-3119(16)61593-9

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (10): 2231-2238.DOI: 10.1016/S2095-3119(16)61593-9

收稿日期:2016-12-13 出版日期:2017-10-20 发布日期:2017-09-30

Functional characterization of (E)-β-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin

LI Feng-qi^{1, 2}, FU Ning-ning¹, ZHOU Jing-jiang³, WANG Gui-rong²

¹ Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R.China
² Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
³ Department of Biological Chemistry and Crop Protect, Rothamsted Research, Harpenden AL5 2JQ, UK

Received:2016-12-13 Online:2017-10-20 Published:2017-09-30
Contact: Correspondence WANG Gui-rong, E-mail: grwang@ippcaas.cn
About author:LI Feng-qi, E-mail: pandit@163.com
Supported by:
This work was funded by the International Science and Technology Cooperation Program of China (2013DFG32230) and the Major Project of Genetically Modified Organisms Breeding, China (2016ZX08010005).

摘要/Abstract

Abstract: (E)-β-Caryophyllene is a sesquiterpene compound widely distributed in plants and functions in plant defence. However, little is known about the sequence and function of (E)-β-caryophyllene synthase in lima bean (Phaseolus lunatus). Here, we report a new full-length cDNA (PlCAHS) encoding (E)-β-caryophyllene synthase, a possible key enzyme of plant defence. The cDNA of PlCAHS contains an open reading frame of 1 761 bp, encoding a protein of 586 amino acids with a predicted mass of 67.95 kDa. The deduced amino acid sequence shows 52% identity with sesquiterpene synthase MtCAHS of Medicago truncatula. Based on phylogenetic analysis, PlCAHS is classified as the terpene synthases (TPS)-a subfamily. The recombinant enzyme, expressed in Escherichia coli, catalysed the formation of a major product (E)-β-caryophyllene (82%) and a minor product α-humulene (18%) from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis found that the PlCAHS transcript was significantly up-regulated in leaves after treatment with spider mites and alamethicin (ALA), suggesting its ecological function in plant defence.

Key words: plant defence , terpene synthases , (E)-β-caryophyllene , lima bean

. [J]. Journal of Integrative Agriculture, 2017, 16(10): 2231-2238.

LI Feng-qi, FU Ning-ning, ZHOU Jing-jiang, WANG Gui-rong. Functional characterization of (E)-β-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin[J]. Journal of Integrative Agriculture, 2017, 16(10): 2231-2238.

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Functional characterization of (E)-β-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin

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