Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea

doi:10.1016/S2095-3119(16)61594-0

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (10): 2239-2245.DOI: 10.1016/S2095-3119(16)61594-0

收稿日期:2016-12-19 出版日期:2017-10-20 发布日期:2017-09-30

Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea

CHEN Guo-hua¹, TIAN Xue-liang², WANG Dian-dong³, LING Jian¹, MAO Zhen-chuan¹, YANG Yu-hong¹, XIE Bing-yan¹

¹ Institute of Vegetable and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture, Beijing 100081, P.R.China
² College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang 453003, P.R.China
³ Life Science and Technology Institute, Yangtze Normal University, Fuling 408100, P.R.China

Received:2016-12-19 Online:2017-10-20 Published:2017-09-30
Contact: Correspondence XIE Bing-yan, Tel: +86-10-82109546, Fax: +86-10-62174123, E-mail: xiebingyan@caas.cn
About author:CHEN Guo-hua, E-mail: chenguohua01@caas.cn;
Supported by:
This research was supported by the National Natural Science Foundation of China (30900926), funded by the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the China Agriculture Research System (CARS-23-D01).

摘要/Abstract

Abstract: The expression of mitogen-activated protein kinase (mapk) double-stranded RNA in cucumber is effective in controlling infestations of the root-knot nematode Meloidogyne incognita. However, little is known about the ecological effects of transgenic plants. Here, we analyzed the diversity of 16S rDNA genes derived from the rhizosphere archaea of transgenic cucumber plants as an indicator of ecological change. A total of 17 and 18 operational taxonomic units were detected in the rhizospheres of non-transgenic cucumber and mapk dsRNA-expressing plants, respectively. No significant difference was observed between the two groups according to Shannon and Simpson indices. In soil samples of the two rhizospheres, the dominant group was Crenarchaeota at the phylum level, with Staphylothermus, Methanococcus, Pyrodictium and Sulfolobus the abundant taxa at the genus level. These results suggest that expressing mapk double-stranded (ds) RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea, and provide powerful evidence for the ecological safety of transgenic cucumber expressing mapk dsRNA.

Key words: cucumber , 16S rDNA , diversity , rhizosphere archaea , mapk dsRNA

. [J]. Journal of Integrative Agriculture, 2017, 16(10): 2239-2245.

CHEN Guo-hua, TIAN Xue-liang, WANG Dian-dong, LING Jian, MAO Zhen-chuan, YANG Yu-hong, XIE Bing-yan. Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea[J]. Journal of Integrative Agriculture, 2017, 16(10): 2239-2245.

参考文献

Abreu C, Jurgens G, De Marco P, Saano A, Bordalo A A. 2001. Crenarchaeota and Euryarchaeota in temperate estuarine sediments. Journal of Applied Microbiology, 90, 713–718.

Aller J Y, Kemp P F. 2008. Are Archaea inherently less diverse than Bacteria in the same environments? Fems Microbiology Ecology, 65, 74–87.

Bintrim S B, Donohue T J, Handelsman J, Roberts G P, Goodman R M. 1997. Molecular phylogeny of Archaea in soil. Proceedings of the National Academy of Sciences of the United States of America, 94, 277–282.

Borneman J, Triplett E W. 1997. Molecular microbial diversity in soils from eastern Amazonia: Evidence for unusual microorganisms and microbial population shifts associated with deforestation. Applied & Environmental Microbiology, 63, 2647–2653.

Charlton W L, Harel H Y M, Bakhetia M, Hibbarda J K, Atkinsona H J, McPhersona M J. 2010. Additive effects of plant expressed double-stranded RNAs on root-knot nematode development. International Journal for Parasitology, 40, 855–864.

Chen G H, Xiao L, Zhang S Q, Yang Y H, Xie B Y. 2008. Analysis of silencing MAPK by RNA interference of root-knot nematode. Acta Phytopathologica Sinica, 38, 509–513. (in Chinese)

Clement M, Ketelaar T, Rodiuc N, Banora M Y, Smertenko A, Engler G, Abad P, Hussey P J, Engler J D. 2009. Actin-depolymerizing factor2-mediated actin dynamics are essential for root-knot nematode infection of Arabidopsis. The Plant Cell, 9, 2963–2979.

DeLong E F. 1992. Archaea in coastal marine environments. Proceedings of the National Academy of Sciences of the United States of America, 89, 5685–5689.

Elling A A. 2013. Major Emerging problems with minor meloidogyne species. Phytopathology, 103, 1092–1102.

Fairbairn D J, Cavallaro A S, Bernard M, Mahalingaiyer J, Graham M W. 2007. Host-delivered RNAi: An effective strategy to silence genes in plant parasitic nematodes. Planta, 226, 1525–33.

Fan H X, Fairley D J, Rensing C, Pepper I L, Wang G J. 2006. Identification of similar non-thermophilic Crenarchaeota in four Chinese and American pristine soils. Biodiversity Science, 14, 181–187.

Hamamouch N, Li C, Hewezi T, Baum T J, Mitchum M G, Hussey R S, Vodkin L O , Davis E L. 2012. The interaction of the novel 30c02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism. Journal of Experimental Botany, 63, 3683–3695.

He J Z, Shen J P, Zhang LM, Zhu Y G, Zheng Y M, Xu M G, Di H J. 2007. Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices. Environmental Microbiology, 9, 2364–2374.

Huang G, Allen R, Davis E L, Baum T J, Hussey R S. 2006. Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene. Proceedings of the National Academy of Sciences of the United States of America, 103, 14302–14306.

Huber J A, Butterfield D A, Baross J A. 2002. Temporal changes in archaeal diversity and chemistry in a mid-ocean ridge subseafloor habitat. Applied & Environmental Microbiology, 68, 1585–1594.

Jackson C R, Liew K C, Yule C M. 2009. Structural and functional changes with depth in microbial communities in a tropical Malaysian peat swamp forest. Microbial Ecology, 57, 402–412.

Jurgens G, Lindstrom K, Saano A. 1997. Novel group within the kingdom crenarchaeota from boreal forest soil. Applied & Environmental Microbiology, 63, 803–805.

Jurgens G, Saano A. 1999. Diversity of soil archaea in boreal forest before, and after clear-cutting and prescribed burning 1. Fems Microbiology Ecology, 29, 205–213.

Kamath R S, Martinez-Campos M, Zipperlen P, Fraser A G, Ahringer J. 2001. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biolog, 2, 1–10.

Klink V P, Matthews B F. 2009. Emerging approaches to broaden resistance of soybean to soybean cyst nematode as supported by gene expression studies. Plant Physiology, 151, 1017–1022.

Kyndt T, Ji H, Vanholme B, Gheysen G. 2013. Transcriptional silencing of RNAi constructs against nematode genes in Arabidopsis. Nematology, 15, 519–528.

Lozupone C, Knight R. 2005. UniFrac: A new phylogenetic method for comparing microbial communities. Applied and Environmental Microbiology, 71, 8228–8235.

Nicol G W, Glover L A, Prosser J I. 2003. The impact of grassland management on archaeal community structure in upland pasture rhizosphere soil. Environmental Microbiology, 5, 152–162.

Nicol G W, Leininger S, Schleper C, Prosser J I. 2008. The influence of soil pH on the diversity abundance and transcriptional activity of ammonia oxidizing archaea and bacteria. Environmental Microbiology, 10, 2966–2978.

Nicol G W, Webster G, Glover L A, Prosser J I. 2004. Differential response of archaeal and bacterial communities to nitrogen inputs and pH changes in upland pasture rhizosphere soil. Environmental Microbiology, 6, 861–867.

Pace N R. 1997. A molecular view of microbial diversity and the biosphere. Science, 276, 734–740.

Pesaro M, Widmer F. 2006. Identification of novel Crenarchaeota and Euryarchaeota clusters associated with different depth layers of a forest soil. Fems Microbiology Ecology, 42, 89–98.

Rani A, Porwal S, Sharma R, kapley A, Purohit H J, Kalia V C. 2008. Assessing microbial diversity by culture-dependent and independent approaches for efficient functioning of effluent treatment plants. Bioresource Technology, 99, 7098–7107.

Sandaa R A, Enger, Torsvik V. 1999. Abundance and diversity of Archaea in heavy-metal-contaminated soils. Applied and Environmental Microbiology, 65, 3293–3297.

Schloss P D, Handelsman J. 2005. Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Applied and Environmental Microbiology, 7, 1501–1506.

Simon H M, Dodsworth J A, Goodman R M. 2000. Crenarchaeota colonize terrestrial plant roots. Environmental Microbiology, 2, 495–505.

Sindhu A S, Maier T R, Mitchum M G, Hussey R S, Davis E L, Baum T J. 2009. Effective and specific in planta RNAi in cyst nematodes: Expression interference of four parasitism genes reduces parasitic success. Journal of Experimental Botany, 60, 315–324.

Takahashi M, Iwasaki H, Inoue H, Takahashi K. 2002. Reverse genetic analysis of the Caenorhabditis elegans 26s proteasome subunits by RNA interference. Biological Chemistry, 383, 1263–1266.

Urwin P, Lilley C J, Atkinson H J. 2002. Ingestion of double-stranded RNA by preparasitic juvenile cyst nematodes leads to RNA interference. Molecular Plant-Microbe Interactions, 15, 747–752.

Wachinger G, Fiedler S, Zepp K, Gattinger A, Sommer M, Roth K. 2000. Variability of soil methane production on the micro-scale: Spatial association with hot spots of organic material and Archaeal populations. Soil Biology and Biochemistry, 32, 1121–1130.

Wang Y, Gu X F, Zhang S P, Miao H, Chen G H, Xie B Y. 2014. Transformation of RNAi vector in cucumber (Cucumis sativus) in vitro by Agrobacterium tumefaciens-mediated transfection. Chinese Bulletin of Botany, 49, 183–189. (in Chinese)

Widmann C, Gibson S, Jarpe M B. 1999. Mitogen-activated protein kinase: Conservation of a three-kinase module from yeast to human. Physiological Reviews, 79, 143–180.

Xue B, Hamamouch N, Li C, Huang G M, Hussey R S, Baum T J, Davis E L. 2013. The 8D05 parasitism gene of Meloidogyne incognita is required for successful infection of host roots. Phytopathology, 103, 175–181.

Yadav B C, Veluthambi K, Subramaniam K. 2006. Host-generated double stranded RNA induces RNAi in plant-parasitic nematodes and protects the host from infection. Molecular and Biochemical Parasitology, 148, 219–222.

Yang Y H. 2011. Advances on the effects of genetically modified crops on soil microbial community and main countermeasures of their approaches. Journal of Agricultural Biotechnology, 19, 1–8.

Zamore P D, Tuschl T, Sharp P A, Bartel D. 2000. RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell, 101, 25–34.

Zhao Z X, Lu X F, Chen G H, Mao Z C, Yang Y H, Liu E M, Xie B Y. 2011 Diversity analysis of archaeal and fungal communities in adjacent cucumber root soil samples in greenhouse by small-subunit rRNA gene cloning. Chinese Journal of Biotechnology, 27, 41–51. (in Chinese)

Zheng J, Peng D, Chen L, Liu L, Chen F, Xu M, Ruan L, Sun M. 2016. The Ditylenchus destructor genome provides new insights into the evolution of plant parasitic nematodes. Proceedings of the Royal Society (B: Biological Sciences), 283, 1835.

Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea

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