A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection

doi:10.1016/S2095-3119(16)61382-5

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (11): 2556-2562.DOI: 10.1016/S2095-3119(16)61382-5

收稿日期:2016-02-24 出版日期:2016-11-04 发布日期:2016-11-04

A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection

ZHAO Xu¹, SHAN Ya-nan¹, ZHAO Yan¹, WANG Ai-rong¹, WANG Zong-hua^{1, 2}

¹ Fujian University Key Laboratory for Functional Genomics of Plant Fungal Pathogens, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China
² Key Laboratory of Bio-Pesticide and Chemistry Biology, Ministry of Education, Fuzhou 350002, P.R.China

Received:2016-02-24 Online:2016-11-04 Published:2016-11-04
Contact: WANG Ai-rong, E-mail: arxg3000@163.com; WANG Zong-hua, E-mail: wangzh@fafu.edu.cn
Supported by:
This work was supported by grants from the Special Fund for Agro-scientific Research in the Public Interest, China (201103016), and the Fujian Provincial Science Foundation, China (2013J06007).

摘要/Abstract

Abstract: Plant defense responses against penetration or colonization of pathogens are mediated by activation and repression of a large array of genes. Host endogenous small RNAs are essential in gene expression reprogramming process. We identified a new Arabidopsis microRNA (miRNA) ath-miR38-3P by high-throughput sequencing and further confirmed it by Northern blot assay. Interestingly, ath-miR38-3P was highly induced after infection of the pathogen Sclerotinia sclerotiorum. Further analysis based on the miRNA target database demonstrated that ath-miR38-3P might target to five putative genes: AT2G03140, AT5G59430, AT5G66320, AT1G36620 and AT3G03820. To confirm the target, we conducted the quantitative real-time PCR to observe the expression pattern of each candidate gene. The results showed that only AT3G03820 was down-regulated after inoculation of S. sclerotiorum. In addition, overexpression of ath-miR38-3P down-regulates AT3G03820, suggesting AT3G03820 might represent the target for ath-miR38-3P. Our results may provide the useful information for further studying the biological function of a novel ath-miR38-3P and its targets in Arabidopsis-Sclerotinia interaction.

Key words: miRNA , Arabidopsis thaliana , Sclerotinia sclerotiorum , miRNA-target gene

ZHAO Xu, SHAN Ya-nan, ZHAO Yan, WANG Ai-rong, WANG Zong-hua. [J]. Journal of Integrative Agriculture, 2016, 15(11): 2556-2562.

ZHAO Xu, SHAN Ya-nan, ZHAO Yan, WANG Ai-rong, WANG Zong-hua. A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection[J]. Journal of Integrative Agriculture, 2016, 15(11): 2556-2562.

参考文献

Bai M Y, Shang J X, Oh E, Fan M, Bai Y, Zentella R, Sun T P, Wang Z Y. 2012. Brassinosteroid, gibberellin and phytochrome impinge on a common transcription module in Arabidopsis. Nature Cell Biololgy, 14, 810–817.

Boland G J, Hall R. 1994. Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 16, 93–108.

Campo S, Peris C, Sire C, Moreno A B, Donaire L, Zytnicki M, Notredame C, Llave C, San Segundo B. 2013. Identification of a novel microRNA (miRNA) from rice that targets an alternatively spliced transcript of the Nramp6 (Natural resistance-associated macrophage protein 6) gene involved in pathogen resistance. The New phytologist, 199, 212–227.

Cunha W G, Tinoco M L P, Pancoti H L, Ribeiro R E, Aragão F J L. 2010. High resistance to Sclerotinia sclerotiorum in transgenic soybean plants transformed to express an oxalate decarboxylase gene. Plant Pathology, 59, 654–660.

Dai X, Zhao P X. 2011. psRNATarget: A plant small RNA target analysis server. Nucleic Acids Research, 39, 155–159.

Dias B B A, Cunha W G, Morais L S, Vianna G R, Rech E L, de Capdeville G, Aragão F J L. 2006. Expression of an oxalate decarboxylase gene from Flammulina sp. in transgenic lettuce (Lactuca sativa) plants and resistance to Sclerotinia sclerotiorum. Plant Pathology, 55, 187–193.

Gil P, Green P. 1997. Regulatory activity exerted by the SAUR-AC1 promoter region in transgenic plants. Plant Molecular Biology, 34, 803–808.

Hagen G, Guilfoyle T. 2002. Auxin-responsive gene expression: Genes, promoters and regulatory factors. Plant Molecular Biology, 49, 373–385.

He X F, Fang Y Y, Feng L, Guo H S. 2008. Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR-NBS-LRR class R gene-derived novel miRNA in Brassica. Febs Letters, 582, 2445–2452.

Jain M, Tyagi A K, Khurana J P. 2006. Genome-wide analysis, evolutionary expansion, and expression of early auxin-responsive SAUR gene family in rice (Oryza sativa). Genomics, 88, 360–371.

Katiyar-Agarwal S, Jin H. 2010. Role of small RNAs in host-microbe interactions. Annual Review of Phytopathology, 48, 225–246.

Lee R C, Feinbaum R L, Ambros V. 1993. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell, 75, 843–854.

Li Y, Lu Y G, Shi Y, Wu L, Xu Y J, Huang F, Guo X Y, Zhang Y, Fan J, Zhao J Q, Zhang H Y, Xu P Z, Zhou J M, Wu X J, Wang P R, Wang W M. 2014. Multiple rice microRNAs are involved in immunity against the blast fungus Magnaporthe oryzae. Plant Physiology, 164, 1077–1092.

Liu F, Wang M, Wen J, Yi B, Shen J, Ma C, Tu J, Fu T. 2015. Overexpression of barley oxalate oxidase gene induces partial leaf resistance to Sclerotinia sclerotiorum in transgenic oilseed rape. Plant Pathology, 64, 1407–1416.

Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2–ΔΔCT method. Methods, 25, 402–408.

McClure B, Guilfoyle T. 1987. Characterization of a class of small auxin-inducible soybean polyadenylated RNAs. Plant Molecular Biology, 9, 611–623.

Navarro L, Dunoyer P, Jay F, Arnold B, Dharmasiri N, Estelle M, Voinnet O, Jones J D G. 2006. A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science, 312, 436–439.

Oh E, Zhu J Y, Wang Z Y. 2012. Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses. Nature Cell Biology, 14, 802–809.

Spartz A K, Ren H, Park M Y, Grandt K N, Lee S H, Murphy A S, Sussman M R, Overvoorde P J, Gray W M. 2014. SAUR Inhibition of PP2C-D phosphatases activates plasma membrane H+-ATPases to promote cell expansion in Arabidopsis. The Plant Cell, 26, 2129–2142.

Wang Z, Mao H, Dong C, Ji R, Cai L, Fu H, Liu S. 2009. Overexpression of Brassica napus MPK4 enhances resistance to Sclerotinia sclerotiorum in oilseed rape. Molecular Plant-Microbe Interactions, 22, 235–244.

Williams B, Kabbage M, Kim H J, Britt R, Dickman M B. 2011. Tipping the balance: Sclerotinia sclerotiorum secreted oxalic acid suppresses host defenses by manipulating the host redox environment. Plos Pathogens, 7, e1002107.

Zhang X, Zhao H, Gao S, Wang W C, Katiyar-Agarwal S, Huang H D, Raikhel N, Jin H. 2011. Arabidopsis argonaute 2 regulates innate immunity via miRNA393*-mediated silencing of a golgi-localized SNARE gene, MEMB12. Molecular Cell, 42, 356–366.

A novel Arabidopsis miRNA, ath-miR38-3P, is involved in response to Sclerotinia sclerotiorum infection

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics