Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

doi:10.1016/S2095-3119(16)61432-6

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (11): 2563-2570.DOI: 10.1016/S2095-3119(16)61432-6

收稿日期:2016-03-11 出版日期:2016-11-04 发布日期:2016-11-04

Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

DENG Wei, YANG Qian, JIAO Hong-tao, ZHANG Yong-zhi, LI Xue-feng, ZHENG Ming-qi

Department of Applied Chemistry, China Agricultural University, Beijing 1000193, P.R.China

Received:2016-03-11 Online:2016-11-04 Published:2016-11-04
Contact: ZHENG Ming-qi, Tel: +86-10-62734294, E-mail: mqzheng@cau.edu.cn
Supported by:
The project was sponsored by the Special Fund for Agro-scientific Research in the Public Interest, China (201303031).

摘要/Abstract

Abstract: Flixweed (Descurainia sophia L.) is a problematic and widespread weed in winter wheat fields and has been controlled by tribenuron-methyl for more than twenty years in China. In this study, a flixweed accession (Hebei 25, HB25) with an Asp-376-Glu mutation in acetohydroxy acid synthase (AHAS) was identified and purified. The purified HB25 accession (pHB25) developed 758.1-fold resistance to tribenuron-methyl and exhibited obvious cross-resistance to four AHAS-inhibiting herbicides. The resistant/susceptible (R/S) ratios of 50% plant growth reduction (GR₅₀) to herbicides of halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 346.1, 15.7, 8.1 and 7.1, respectively. The reduced AHAS sensitivities to four different AHAS-inhibiting herbicides, which were caused by the Asp-376-Glu mutation, were responsible for the resistance and cross-resistance to AHAS-inhibiting herbicides. The R/S ratios of 50% inhibition of AHAS activity (I₅₀) to tribenuron-methyl, halosulfuron-methyl, flumetsulam, imazethapyr and pyribenzoxim were 844.5, 532.9, 74.5, 13.3 and 5.5, respectively. The results of AHAS activity in vitro were highly correlated with that of whole-plant response experiments.

Key words: flixweed , AHAS , cross-resistance , Asp-376-Glu mutation

DENG Wei, YANG Qian, JIAO Hong-tao, ZHANG Yong-zhi, LI Xue-feng, ZHENG Ming-qi. [J]. Journal of Integrative Agriculture, 2016, 15(11): 2563-2570.

DENG Wei, YANG Qian, JIAO Hong-tao, ZHANG Yong-zhi, LI Xue-feng, ZHENG Ming-qi. Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS[J]. Journal of Integrative Agriculture, 2016, 15(11): 2563-2570.

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Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS

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