Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates

doi:10.1016/S2095-3119(16)61338-2

Journal of Integrative Agriculture ›› 2017, Vol. 16 ›› Issue (03): 713-724.DOI: 10.1016/S2095-3119(16)61338-2

收稿日期:2016-01-14 修回日期:2016-04-04 出版日期:2017-03-20 发布日期:2017-03-07

Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates

JIANG Lin-lin^{1, 2*}, HAN Guang-ming^3*, LAN Yu¹, LIU Sai-nan¹, GAO Ji-ping², YANG Xu¹, MENG Jun¹, CHEN Wen-fu^{1, 2}

¹Liaoning Biochar Engineering & Technology Research Center, Shenyang Agricultural University, Shenyang 110866, P.R.China
²Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, P.R.China
³Industrial Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China

Received:2016-01-14 Revised:2016-04-04 Online:2017-03-20 Published:2017-03-07
Contact: MENG Jun, E-mail: mengjun1217@163.com; CHEN Wen-fu, E-mail: wfchen5512@163.com
About author:JIANG Lin-lin, E-mail: jiangll19830223@163.com; HAN Guang-ming, E-mail: mrhan888@hotmail.com
Supported by:
This research was funded by the National Natural Science Foundation of China (41401325 and 31501250), the Key Technologied R&D Program of China during the 12th Five-Year Plan period (2014BAD02B06-02), the Special Fund for Agro-scientific Research in the Public Interest, China (201303095), the Excellent Talent Support Program of Ministry of Liaoning Education, China.

摘要/Abstract

Abstract: Biochar has been shown to influence soil microbial communities in terms of their abundance and diversity. However, the relationship among microbial abundance, structure and C metabolic traits is not well studied under biochar application. Here it was hypothesized that the addition of biochar with intrinsic properties (i.e., porous structure) could affect the proliferation of culturable microbes and the genetic structure of soil bacterial communities. In the meantime, the presence of available organic carbon in biochar may influence the C utilization capacities of microbial community in Biolog Eco-plates. A pot experiment was conducted with differenct biochar application (BC) rates: control (0 t ha^–1), BC1 (20 t ha^–1) and BC2 (40 t ha^–1). Culturable microorganisms were enumerated via the plate counting method. Bacterial diversity was examined using denaturing gradient gel electrophoresis (DGGE). Microbial capacity in using C sources was assessed using Biolog Eco-plates. The addition of biochar stimulated the growth of actinomyces and bacteria, especially the ammonifying bacteria and azotobacteria, but had no significant effect on fungi proliferation. The phylogenetic distribution of the operational taxonomic units could be divided into the following groups with the biochar addition: Firmicutes, Acidobacteria, Gemmatimonadetes, Actinobacteria, Cyanobacteria and α-, β-, γ- and δ-Proteobacteria (average similarity >95%). Biochar application had a higher capacity utilization for L-asparagine, Tween 80, D-mannitol, L-serine, γ-hydroxybutyric acid, N-acetyl-D-glucosamine, glycogen, itaconic acid, glycyl-L-glutamic acid, α-ketobutyricacid and putrescine, whereas it had received decreased capacities in using the other 20 carbon sources in Biolog Eco-plates. Redundancy analysis (RDA) revealed that the physico-chemical properties, indices of bacterial diversity, and C metabolic traits were positively correlated with the appearance of novel sequences under BC2 treatment. Our study indicates that the addition of biochar can increase culturable microbial abundance and shift bacterial genetic structure without enhancing their capacities in utilizing C sources in Biolog Eco-plates, which could be associated with the porous structure and nutrients from biochar.

Key words: biochar, soil microbial community, DGGE Biolog Eco-plates

JIANG Lin-lin, HAN Guang-ming, LAN Yu, LIU Sai-nan, GAO Ji-ping, YANG Xu, MENG Jun, CHEN Wen-fu. [J]. Journal of Integrative Agriculture, 2017, 16(03): 713-724.

JIANG Lin-lin, HAN Guang-ming, LAN Yu, LIU Sai-nan, GAO Ji-ping, YANG Xu, MENG Jun, CHEN Wen-fu. Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates[J]. Journal of Integrative Agriculture, 2017, 16(03): 713-724.

参考文献

Babin D, Ding G C, Pronk G J, Heister K, Kögel-Knabner I, Smalla K. 2013. Metal oxides, clay minerals and charcoal determine the composition of microbial communities in matured arti?cial soils and their response to phenanthrene. FEMS Microbiology Ecology, 86, 3–14.
Ball P N, Mackenzie M D, Deluca T H, Holben W E. 2010. Wildfire and charcoal enhance nitrification and ammonium-oxidizing bacterial abundance in dry montane forest soils. Journal of Environmental Quality, 39, 1243–1253.
Bassam B J, Caetanoanolles G, Gresshoff P M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196, 80–83.
Beesley L, Moreno-Jiménez E, Gomez-Eyles J L, Harris E, Robinson B, Sizmur T. 2011. A review of biochars’ potential role in the remediation, revegetation and restoration of contaminated soils. Environmental Pollution, 159, 3269–3282.
Brewer C E, Schmidt-Rohr K, Satrio J A, Brown R C. 2009. Characterization of biochar from fast pyrolysis and gasification systems. Environmental Progress and Sustainable Energy, 28, 386–396.
Brussaard L. 1997. Biodiversity and ecosystem functioning in soil. AMBIO, 26, 563–570.
Bruun E W, Petersen C T, Hansen E, Hauggaard-Nielsen H. 2014. Biochar amendment to coarse sandy subsoil improves root growth and increases water retention. Soil Use and Management, 30, 109–118.
Chen Z Y, Zhang J, Huang D F. 2003. Plant disease biocontrol bacillus antibacterial mechanism and genetic improvement research. Chinese Plant Pathology, 33, 97–103. (in Chinese)
Craig R A, Leo M C, Tim J C, Mark F, Alison S, Robert A H, Robert R S. 2011. Biochar induced soil microbial community change: Implications for biogeochemical cycling of carbon, nitrogen and phosphorus. Pedobiologia, 54, 309–320.
Covacevich F, Marino M A, Echeverrica H E. 2006. The phosphorus source determines the arbuscular mycorrhizal potential and the native mycorrhizal colonization of tall fescue and wheatgrass. European Journal of Soil Biology, 42, 127–138.
Deanna D N, Claudia W R, Kari E D. 2014. Abundance and gene expression in nitri?er and denitri?er communities associated with a ?eld scale spring thaw N₂O ?ux event. Soil Biology & Biochemistry, 73, 1–9.
Dempster D N, Gleeson D B, Solaiman Z M , Jones D L, Murphy D V. 2012. Decreased soil microbial biomass and nitrogen mineralisation with Eucalyptus biochar addition to a coarse textured soil. Plant Soil, 354, 311–324.
Dodds W K, Gudder D A, Mollenhauer D. 1995. The ecology of Nostoc. Journal of Phycology, 31, 2–18.
Downie A, Crosky A, Munroe P. 2009. Physical properties of biochar. In: Lehmann J, Joseph S, eds., Biochar for Environmental Management: Science and Technology. Earthscan, London. pp. 13–32.
Du S, Gao X Z. 2006. Soil Analysis Technical Apecifications. China Agriculture Press, China. pp. 67–68. (in Chinese)
Fawaz M N. 2013. Revealing the ecological role of gemmatimonadetes through cultivation and molecular analysis of agricultural soils. MSc thesis, University of Tennessee, USA.
Garland J, Mills A. 1991. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilisation. Applied Environmental Microbiology, 57, 2351–2359.
Gomes N C M, Heuer H, Schönfeld J, Costa R, Mendonça-Hagler L, Smalla K. 2001. Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis. Plant and Soil, 232, 167–180.
Graber E R, Harel Y M, Kolton M, Cytryn E, Silber A, David D R, Tsechansky L, Borenshtein M, Elad Y. 2010. Biochar impact on development and productivity of pepper and tomato grown in fertigated soilless media. Plant and Soil, 337, 481–496.
Grossman J M, O’Neill B E, Tsai S M, Liang B, Neves E, Lehmann J, Thies J E. 2010. Amazonian anthrosols support similar microbial communities that differ distinctly from those extant in adjacent, unmodi?ed soils of the same mineralogy. Microbial Ecology, 60, 192–205.
Hale S E, Meynet P, Davenport R J, Martin J D, Werner D. 2010.Changes in polycyclic aromatic hydrocarbon availability in River Tyne sediment following bioremediation treatments or activated carbon amendment. Water Research, 44, 4529–4536.
Helliwell J R, Miller A J , Whalley W R, Mooney S J , Sturrock C J. 2014. Quantifying the impact of microbes on soil structural development and behaviour in wet soils. Soil Biology & Biochemistry, 74, 138–147.
Jin H. 2010. Characterization of microbial life colonizing biochar and biochar-amended soils. Ph D thesis, Cornell University, Ithaca, NY.
Laird D A. 2008. The charcoal vision: A win-win-win scenario for simultaneously producing bioenergy, permanently sequestering carbon, while improving soil and water quality. Agronomy Journal, 100, 178–181.
Lehmann J, Matthias C R, Janice T, Caroline A M, William C H, David C. 2011. Biochar effects on soil biota - A review. Soil Biology & Biochemistry, 43, 1812–1836.
Lehmann J, Stephen J. 2009. Biochar for environmental management: An introduction. In: Lehmann J D, Joseph S, eds., Biochar for Environmental Management: Science and Technology. Earthscan, London. pp. 1–12.
Liang B, Lehmann J, Solomon D, Kinyangi J, Grossman J, O’Neill B, Skjemstad J O, Thies J, Luizão F J, Petersen J, Neves E G. 2006. Black carbon increases cation exchange capacity in soils. Soil Science Society of America Journal, 70, 1719–1730.
Lin Q M, Wu Y G. 1999. Fumigation method for improve the soil microbial biomass carbon determination. Chinese Journal of Ecology, 18, 63–66. (in Chinese)
Liu Y, Li M, Zheng J W, Li L Q, Zhang X H, Zheng J F, Pan G X, Yu X Y, Wang J F. 2014. Short-term responses of microbial community and functioning to experimental CO₂ enrichment and warming in a Chinese paddy ?eld. Soil Biology & Biochemistry, 77, 58–68.
Lu W W , Ding W X , Zhang J H , Li Y, Luo J F, Nanthi B, Xie Z B. 2015. Biochar suppressed the decomposition of organic carbon in a cultivated sandy loam soil: A negative priming effect. Soil Biology & Biochemistry, 76, 12–21.
Major J, Lehmann J, Rondon M, Goodale C. 2010. Fate of soil-applied black carbon: Downward migration, leaching and soil respiration. Global Change Biology, 16, 1366–1379.
Muyzer G, De Waal E C, Uitterlinden A G. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Applied Environmental Microbiology, 59, 695–700.
Ng E L, Patti A F, Rose M T, Schefe C R, Wilkinson K, Smernik R J, Cavagnaro T R. 2013. Does the chemical nature of soil carbon drive the structure and functioning of soil microbial communities? Soil Biology & Biochemistry, 70, 54–61.
Olsen S R, Cole C V, Watanable F S, Dean L A. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. United States Department of Agriculture Circular, 939, 1–19.
Putman M, Burton R, Nahm M H. 2005. Simplified method to automatically count bacterial colony forming unit. Journal of Immunological Methods, 302, 99–10.
Quilliam R S, Glanville H C, Wade S C, Jones D L. 2013. Life in the ‘charosphere’ - Does biochar in agricultural soil provide a signi?cant habitat for microorganisms? Soil Biology and Biochemistry, 65, 287–293.
Rutigliano F A, Romano M, Marzaioli R, Baglivo I, Baronti S, Miglietta F, Castaldi S. 2014. Effect of biochar addition on soil microbial community in a wheat crop. European Journal of Soil Biology, 60, 9–15.
Samadhi M C, Sarath C J, Ernesto P R. 2006. Identification and analysis of DNA-binding transcription factors in Bacillus subtilis and other Firmicutes - A genomic approach. BMC Genomics, 7, doi: 10.1186/1471-2164-7-147
Samonin V V, Elikova E E. 2004. A study of the adsorption of bacterial cells on porous materials. Microbiology, 73, 696–701.
Smalla K, Wieland G, Buchner A, Zock A, Parzy J, Kaiser S, Roskot N, Heuer H, Berg G. 2001. Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: Plant-dependent enrichment and seasonal shifts revealed. Applied Environmental Microbiology, 67, 4742–4751.
Subbiah B V, Asija G L . 1956. A rapid procedure for assessment of available nitrogen in soils. Current Science, 31, 196–260.
Sun D Q, Meng J, Chen W F. 2013. Effects of abiotic components induced by biochar on microbial communities. Acta Agriculturae Scandinavica (Section B: Soil & Plant Science), 63, 633–641.
Thies J E, Rillig M C. 2009. Characteristics of biochar: Biological properties. In: Lehmann J, Joseph S, eds., Biochar for Environmental Management. Science and Technology. Earthscan, London. pp. 85–105.
Vance E D, Brooks P C, Jenkionson D S. 1987. An extraction method for measuring soil microbial biomass C. Soil Biology & Biochemistry, 19, 703–707.
Vos M, Wolf A B, Jennings S J, Kowalchuk G A. 2013. Micro-scale determinants of bacterial diversity in soil. FEMS Microbiology Reviews, 37, 936–954.
Warnock D D, Lehmann J, Kuyper T W, Rillig M C. 2007. Mycorrhizal responses to biochar in soil - concepts and mechanisms. Plant Soil, 300, 9–20.
Warnock D D, Mummey D L, McBride B, Major J, Lehmann J, Rillig M C. 2010. In?uences of non-herbaceous biochar on arbuscular mycorrhizal fungal abundances in roots and soils: Results from growth-chamber and ?eld experiments. Applied Soil Ecology, 46, 450–456.
Xing D F, Cheng S A, Logan B E, Regan J M. 2010. Isolation of the exoelectrogenic denitrifying bacterium comamonas denitrificans based on dilution to extinction. Applied Microbiology Biotechnology, 85, 1575–1587.
Young I M, Crawford J W. 2004. Interactions and self-organization in the soil Microbe complex. Science, 304, 1634–1637.
Zhang D X, Pan G X, Wu G, Grace W K, Li L Q, Zhang X H, Zheng J W, Zheng J F, Cheng K, Stephen J, Liu X Y. 2015. Biochar helps enhance maize productivity and reduce greenhouse gas emissions under balanced fertilization in a rainfed low fertility inceptiso. Chemosphere, 142, 106–113.
Zhou J Z, Davey M E, Figueras J B, Rivkina E, Gilichinsky D, Tiedje J M. 1997. Phylogenetic diversity of a bacterial community determined from Siberian tundra soil DNA. Microbiology (UK), 143, 3913–3919.
Zhu Q H, Peng X H, Huang T Q, Xie Z B, Holden N M. 2014. Effect of biochar addition on maize growth and nitrogen use efficiency in acidic red soils. Pedosphere, 24, 699–708.
Van Zwieten L, Kimber S, Morris S, Chan K Y, Downie A, Rust J, Joseph S, Cowie A. 2010. Effects of biochar from slow pyrolysis of papermill waste on agronomic performance and soil fertility. Plant and Soil, 327, 235–246.

Corn cob biochar increases soil culturable bacterial abundance without enhancing their capacities in utilizing carbon sources in Biolog Eco-plates

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics