Functional analysis of a wheat pleiotropic drug resistance gene involved in Fusarium head blight resistance

doi:10.1016/S2095-3119(16)61362-X

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (10): 2215-2227.DOI: 10.1016/S2095-3119(16)61362-X

收稿日期:2015-11-20 出版日期:2016-10-01 发布日期:2016-10-01

Functional analysis of a wheat pleiotropic drug resistance gene involved in Fusarium head blight resistance

WANG Gui-ping^1*, HOU Wen-qian^{1, 2*}, ZHANG Lei³, WU Hong-yan¹, ZHAO Lan-fei¹, DU Xu-ye¹, MA Xin¹, LI An-fei¹, WANG Hong-wei¹, KONG Ling-rang¹

¹ State Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
² College of Life Science, Guizhou Normal University, Guiyang 550001, P.R.China
³ College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, P.R.China

Received:2015-11-20 Online:2016-10-01 Published:2016-10-01
Contact: KONG Ling-rang, Tel: +86-538-8249278, Fax: +86-538-8242226, E-mail: lkong@sdau.edu.cn; WANG Hong-wei, E-mail: wanghongwei@sdau.edu.cn
About author:WANG Gui-ping, E-mail: wgp03165@163.com; HOU Wen-qian, E-mail: houwenqian123@163.com;
Supported by:
This work was supported by the National Natural Science Foundation of China (3315203911, 31471488 and 31171553), the National High-Tech R&D Program of China (2011AA100102 and 2014CB138100), the Transgenic Special Item, China (2016ZX08009-003 and 2016ZX08002003-002), the Natural Science Foundation of Guizhou Province, China (Qianke 2015-2116), the Scientific Research Starting Fund of Doctor of Guizhou Normal University, China, and the Open Subject of National Key Laboratory of Crop Biology, China (2015KF05).

摘要/Abstract

Abstract: The pleiotropic drug resistance (PDR) sub-family of adenosine triphosphate (ATP)-binding cassette (ABC) transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with AePDR7 and BdPDR7. Subcellular localization analysis showed that TaPDR7 was expressed on the plasmalemma. The quantitative real time PCR (RT-PCR) analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol (DON) in spike. When knocking down the transcripts of all TaPDR7 members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that TaPDR7 might play an important role in response to F. graminearum. Although salicylic acid (SA), methyl jasmonate (MeJA) and abscisic acid (ABA) had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of TaPDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid (IAA).

Key words: PDR , FHB , VIGS

WANG Gui-ping, HOU Wen-qian, ZHANG Lei, WU Hong-yan, ZHAO Lan-fei, DU Xu-ye, MA Xin, LI An-fei, WANG Hong-wei, KONG Ling-rang. [J]. Journal of Integrative Agriculture, 2016, 15(10): 2215-2227.

WANG Gui-ping, HOU Wen-qian, ZHANG Lei, WU Hong-yan, ZHAO Lan-fei, DU Xu-ye, MA Xin, LI An-fei, WANG Hong-wei, KONG Ling-rang. Functional analysis of a wheat pleiotropic drug resistance gene involved in Fusarium head blight resistance[J]. Journal of Integrative Agriculture, 2016, 15(10): 2215-2227.

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Functional analysis of a wheat pleiotropic drug resistance gene involved in Fusarium head blight resistance

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