Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (9): 1827-1837.DOI: 10.1016/S2095-3119(15)61034-6

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Endocrine and ovarian responses in water buffalo cows immunized against inhibin and subjected to the Ovsynch protocol

 Abdalla Bahareldin-Ali, QIN Guang-sheng, GUO Ri-hong, Anastasia Tsigkou, TAN Zheng-zhun, HUANG Jian, LI Hui[Author]) AND 1[Journal]) AND year[Order])" target="_blank">LI Hui, LI Hui[Author]) AND 1[Journal]) AND year[Order])" target="_blank">LI Hui, SHI Zhen-dan   

  1. 1、College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R.China
    2 Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Guangxi Buffalo Research Institute, Nanning 530000, P.R.China
    3、Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou 215123, P.R.China
    4、Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China
  • 收稿日期:2014-09-15 出版日期:2015-09-03 发布日期:2015-09-05
  • 通讯作者: SHI Zhen-dan,Mobile: +86-13913888894, E-mail: zdshi@jaas.ac.cn
  • 作者简介:Abdalla Bahareldin-ali, Mobile: +86-13640261354,E-mail: abdalla406@163.com;
  • 基金资助:

    This work was supported by the National Key Technology R&D Program of China (2011BAD19B02-6), the Open Grant of Guangxi Provincial Key Laboratory of Water Buffalo Genetics, Breeding and Reproduction, China (SNKF-2012-04).

Endocrine and ovarian responses in water buffalo cows immunized against inhibin and subjected to the Ovsynch protocol

 Abdalla Bahareldin-Ali, QIN Guang-sheng, GUO Ri-hong, Anastasia Tsigkou, TAN Zheng-zhun, HUANG Jian, LI Hui, LI Hui, SHI Zhen-dan   

  1. 1、College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R.China
    2 Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Guangxi Buffalo Research Institute, Nanning 530000, P.R.China
    3、Department of Biological Sciences, Xi’an Jiaotong-Liverpool University, Suzhou 215123, P.R.China
    4、Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China
  • Received:2014-09-15 Online:2015-09-03 Published:2015-09-05
  • Contact: SHI Zhen-dan,Mobile: +86-13913888894, E-mail: zdshi@jaas.ac.cn
  • About author:Abdalla Bahareldin-ali, Mobile: +86-13640261354,E-mail: abdalla406@163.com;
  • Supported by:

    This work was supported by the National Key Technology R&D Program of China (2011BAD19B02-6), the Open Grant of Guangxi Provincial Key Laboratory of Water Buffalo Genetics, Breeding and Reproduction, China (SNKF-2012-04).

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摘要： The aim of this study was to investigate the feasibility of stimulating ovarian follicle development in order to improve fertility in water buffalo cows by immunization against inhibin. The experiment was carried out in early summer (May) and included 24 multi-parity crossbred Murrah-Swamp buffaloes that were divided into immunized (n=11) and control (n=13) groups. Each immunized cow was administered with a 2-mL immunogen of mineral oil adjuvant containing 2 mg of recombinant inhibin α-subunit fusion protein. The controls were treated with the adjuvant only. All animals received Ovsynch protocol treatment, starting on the day of the antigen administration, and they were artificially inseminated upon behavioral estrus. As a result, all of the immunized buffaloes generated antibodies against inhibin during the experimental period and had higher plasma concentrations of follicle-stimulating hormone (FSH), activin, and estradiol (E2) related to estrous expression. A higher proportion of immunized animals expressed estrus behavior than did the controls (72% vs. 30%, P<0.05). On average, inhibin-immunized buffaloes had significantly more large follicles (≥9 mm in diameter) than the controls (mean±SEM; 1.2±0.1 vs. 0.84±0.1, respectively; P<0.05) and a slightly higher mean total number of follicles (≥2 mm; 11.4±0.7 vs. 9.0±1.1, respectively; P=0.09) and small (2–4 mm) follicles (8.81±0.6 vs. 6.84±1.0, respectively; P=0.12). A higher percentage of cows ovulated in the immunized group than in the control group (91% (10/11) vs. 54% (7/13), respectively; P<0.05). Moreover, inhibin-immunized cows had slightly larger corpus luteum (CL) than the controls 9 days after ovulation and significantly higher (P<0.01) post-ovulation peak plasma progesterone (P4) concentrations. Immunization against inhibin also marginally increased the conception rate 42 days after insemination (45.8% vs. 15.4%; P>0.05). These results demonstrate that immunization against inhibin, coupled with the treatment with the Ovsynch protocol, can constitute a new technique to increase fertility in water buffalo cows.

关键词: inhibin immunization , ovarian responses , luteal function , hormonal patterns , conception rate , water buffalo cows

Abstract: The aim of this study was to investigate the feasibility of stimulating ovarian follicle development in order to improve fertility in water buffalo cows by immunization against inhibin. The experiment was carried out in early summer (May) and included 24 multi-parity crossbred Murrah-Swamp buffaloes that were divided into immunized (n=11) and control (n=13) groups. Each immunized cow was administered with a 2-mL immunogen of mineral oil adjuvant containing 2 mg of recombinant inhibin α-subunit fusion protein. The controls were treated with the adjuvant only. All animals received Ovsynch protocol treatment, starting on the day of the antigen administration, and they were artificially inseminated upon behavioral estrus. As a result, all of the immunized buffaloes generated antibodies against inhibin during the experimental period and had higher plasma concentrations of follicle-stimulating hormone (FSH), activin, and estradiol (E2) related to estrous expression. A higher proportion of immunized animals expressed estrus behavior than did the controls (72% vs. 30%, P<0.05). On average, inhibin-immunized buffaloes had significantly more large follicles (≥9 mm in diameter) than the controls (mean±SEM; 1.2±0.1 vs. 0.84±0.1, respectively; P<0.05) and a slightly higher mean total number of follicles (≥2 mm; 11.4±0.7 vs. 9.0±1.1, respectively; P=0.09) and small (2–4 mm) follicles (8.81±0.6 vs. 6.84±1.0, respectively; P=0.12). A higher percentage of cows ovulated in the immunized group than in the control group (91% (10/11) vs. 54% (7/13), respectively; P<0.05). Moreover, inhibin-immunized cows had slightly larger corpus luteum (CL) than the controls 9 days after ovulation and significantly higher (P<0.01) post-ovulation peak plasma progesterone (P4) concentrations. Immunization against inhibin also marginally increased the conception rate 42 days after insemination (45.8% vs. 15.4%; P>0.05). These results demonstrate that immunization against inhibin, coupled with the treatment with the Ovsynch protocol, can constitute a new technique to increase fertility in water buffalo cows.

Key words: inhibin immunization , ovarian responses , luteal function , hormonal patterns , conception rate , water buffalo cows