Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China

doi:10.1016/S2095-3119(15)61044-9

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (9): 1838-1844.DOI: 10.1016/S2095-3119(15)61044-9

Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China

Wang Shuai, ZHang Meng, LIU Xin-chao, LIn Tao, Yang Han-chun, YUan Shi-shan, ZHao guang-wei, Ia Hassan, Yan Ruo-feng, Song Xiao-kai, XU Li-xin, LI Xiang-rui

1、College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China
2、Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/State Key Laboratory of Agrobiotechnology, Collegeof Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China
3、Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Shanghai 200241, P.R.China

收稿日期:2015-01-16 出版日期:2015-09-03 发布日期:2015-09-05
通讯作者: LI Xiang-rui, Tel/Fax: +86-25-84399000,E-mail: lixiangrui@njau.edu.cn
作者简介:WANG Shuai, Tel: +86-25-84395904, E-mail: tongbaiws1003@163.com;
基金资助:
This work was supported by the Special Fund for Public Welfare Industry of Ministry of Agriculture of China (200903036- 04) and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).

Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China

Wang Shuai, ZHang Meng, LIU Xin-chao, LIn Tao, Yang Han-chun, YUan Shi-shan, ZHao guang-wei, Ia Hassan, Yan Ruo-feng, Song Xiao-kai, XU Li-xin, LI Xiang-rui

1、College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China
2、Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/State Key Laboratory of Agrobiotechnology, Collegeof Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China
3、Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Shanghai 200241, P.R.China

Received:2015-01-16 Online:2015-09-03 Published:2015-09-05
Contact: LI Xiang-rui, Tel/Fax: +86-25-84399000,E-mail: lixiangrui@njau.edu.cn
About author:WANG Shuai, Tel: +86-25-84395904, E-mail: tongbaiws1003@163.com;
Supported by:
This work was supported by the Special Fund for Public Welfare Industry of Ministry of Agriculture of China (200903036- 04) and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).

摘要/Abstract

摘要： The objective of the present investigation was to estimate the prevalence of Toxoplasma gondii infection and co-infection with porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV-2) in pigs in China. A total of 372 tissues or serum samples collected from pigs distributed in 9 provinces/ municipalities of China during the period from February 2011 to November 2012 were assayed for T. gondii antigens and antibodies using enzyme linked immunosorbent assay (ELISA) technique, while the PCR was designed for the detection of the PRRSV, CSFV and PCV-2, respectively. The total positive rate of T. gondii, PRSSV, CSFV and PCV-2 was 9.14% (34/372), 50.00% (186/372), 37.10% (138/372) and 3.23% (12/372), respectively. Among the 34 T. gondii positive samples, 26 samples were simultaneously infected with T. gondii and viruses, while the remaining eight samples were infected with T. gondii alone. In addition, the co-infection rate of T. gondii with PRSSV, T. gondii with PRSSV and CSFV, T. gondii with PRSSV and PCV-2, T. gondii with CSFV and PCV-2, T. gondii with PRSSV, CSFV and PCV-2 was 1.61% (6/372), 4.03% (15/372), 0.27% (1/372), 0.27% (1/372) and 0.81% (3/372), respectively. The results of the present survey revealed that PRRSV and CSFV were the common pathogens co-existing with porcine toxoplasmosis in China, and both of them could increase the chances of T. gondii infection in pig. This is the first report of T. gondii co-infections with viruses in pigs. It is very important to understand the interactions of parasite and virus, and can be used as reference data for the control and prevention of co-infections of T. gondii and viruses in pigs.

关键词: Toxoplasma gondii , coinfection , PRRSV , CSFV , PCV-2 , pig

Abstract: The objective of the present investigation was to estimate the prevalence of Toxoplasma gondii infection and co-infection with porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV-2) in pigs in China. A total of 372 tissues or serum samples collected from pigs distributed in 9 provinces/ municipalities of China during the period from February 2011 to November 2012 were assayed for T. gondii antigens and antibodies using enzyme linked immunosorbent assay (ELISA) technique, while the PCR was designed for the detection of the PRRSV, CSFV and PCV-2, respectively. The total positive rate of T. gondii, PRSSV, CSFV and PCV-2 was 9.14% (34/372), 50.00% (186/372), 37.10% (138/372) and 3.23% (12/372), respectively. Among the 34 T. gondii positive samples, 26 samples were simultaneously infected with T. gondii and viruses, while the remaining eight samples were infected with T. gondii alone. In addition, the co-infection rate of T. gondii with PRSSV, T. gondii with PRSSV and CSFV, T. gondii with PRSSV and PCV-2, T. gondii with CSFV and PCV-2, T. gondii with PRSSV, CSFV and PCV-2 was 1.61% (6/372), 4.03% (15/372), 0.27% (1/372), 0.27% (1/372) and 0.81% (3/372), respectively. The results of the present survey revealed that PRRSV and CSFV were the common pathogens co-existing with porcine toxoplasmosis in China, and both of them could increase the chances of T. gondii infection in pig. This is the first report of T. gondii co-infections with viruses in pigs. It is very important to understand the interactions of parasite and virus, and can be used as reference data for the control and prevention of co-infections of T. gondii and viruses in pigs.

Key words: Toxoplasma gondii , coinfection , PRRSV , CSFV , PCV-2 , pig

Wang Shuai, ZHang Meng, LIU Xin-chao, LIn Tao, Yang Han-chun, YUan Shi-shan, ZHao guang-wei, Ia Hassan, Yan Ruo-feng, Song Xiao-kai, XU Li-xin, LI Xiang-rui. Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China[J]. Journal of Integrative Agriculture, 2015, 14(9): 1838-1844.

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Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China

Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China

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