Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2558-2561.DOI: 10.1016/S2095-3119(14)60896-0

• 论文 • 上一篇    下一篇

Effects of Polysaccharides from Pulsatilla Decoction on the Microvascular Endothelial Glycocalyx

 ZHANG Tao, WU Shuang, SUN Xiong, DUAN Hui-qin , MU Xiang   

  1. Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China
  • 收稿日期:2013-09-29 出版日期:2014-11-06 发布日期:2014-11-14
  • 通讯作者: MU Xiang, Fax/Tel: +86-10-80799515, E-mail: muxiang1109@sina.com
  • 作者简介:ZHANG Tao, Tel: +86-10-80793027, E-mail: zhangtao8008@126.com
  • 基金资助:

    This study was supported by grants from the National Natural Science Foundation of China (31001083), the Scientific Research Program of Beijing Municipal Commission of Education of China (KM201110020014, KZ201110020021) and the Beijing Natural Science Foundation, China (6142004).

Effects of Polysaccharides from Pulsatilla Decoction on the Microvascular Endothelial Glycocalyx

 ZHANG Tao, WU Shuang, SUN Xiong, DUAN Hui-qin , MU Xiang   

  1. Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China
  • Received:2013-09-29 Online:2014-11-06 Published:2014-11-14
  • Contact: MU Xiang, Fax/Tel: +86-10-80799515, E-mail: muxiang1109@sina.com
  • About author:ZHANG Tao, Tel: +86-10-80793027, E-mail: zhangtao8008@126.com
  • Supported by:

    This study was supported by grants from the National Natural Science Foundation of China (31001083), the Scientific Research Program of Beijing Municipal Commission of Education of China (KM201110020014, KZ201110020021) and the Beijing Natural Science Foundation, China (6142004).

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摘要： Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the roles of its polysaccharides still remain unclear. This study aimed to explore effects of polysaccharides from Pulsatilla decoction (PPD) on the microvascular endothelial glycocalyx (eGC). The polysaccharides were extracted from PPD by water extraction and alcohol precipitation method. Mice were administered with PPD for 4 wk, and were then anesthetized with ether inhalation and were fixed by cardiac perfusion with gradient concentration alcian blue solution. The jejunum was sampled and jejunal mucosa was prepared for ultrathin sections by routine method and was analyzed by transmission electron microscope. The results indicated that the eGC was observed as a strong electron-dense smooth linear margin or nonuniform conglomerates coating cell membranes, and PPD significantly increased its thickness from (21.85±1.87) to (28.71±3.61) nm and improved its integrity. This study suggested that PPD may express their biological activities and protect against pathogenic factor damages by influencing the eGC.

关键词: Pulsatilla decoction , herbal polysaccharides , glycocalyx , microvascular endothelial cells , jejunal mucosa , transmission electron microscope

Abstract: Pulsatilla decoction is a famous traditional Chinese herbal formula for clearing heat and treating dysentery of animals or human. To elucidate its mechanism, many active components have been studied, however, the roles of its polysaccharides still remain unclear. This study aimed to explore effects of polysaccharides from Pulsatilla decoction (PPD) on the microvascular endothelial glycocalyx (eGC). The polysaccharides were extracted from PPD by water extraction and alcohol precipitation method. Mice were administered with PPD for 4 wk, and were then anesthetized with ether inhalation and were fixed by cardiac perfusion with gradient concentration alcian blue solution. The jejunum was sampled and jejunal mucosa was prepared for ultrathin sections by routine method and was analyzed by transmission electron microscope. The results indicated that the eGC was observed as a strong electron-dense smooth linear margin or nonuniform conglomerates coating cell membranes, and PPD significantly increased its thickness from (21.85±1.87) to (28.71±3.61) nm and improved its integrity. This study suggested that PPD may express their biological activities and protect against pathogenic factor damages by influencing the eGC.

Key words: Pulsatilla decoction , herbal polysaccharides , glycocalyx , microvascular endothelial cells , jejunal mucosa , transmission electron microscope