Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (3): 561-566.DOI: 10.1016/S2095-3119(14)60832-7

• 论文 • 上一篇    下一篇

Prospective use of bacteriocinogenic Pediococcus pentosaceus as direct-fed microbial having methane reducing potential

 Sanjay Kumar, Sumit S Dagar, Seyed H Ebrahimi, Ravinder K Malik, Ramesh C Upadhyay, AnilK Puniya   

  1. 1、Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
    2、Dairy Cattle Nutrition, National Dairy Research Institute, Karnal 132001, India
    3、Dairy Cattle Physiology, National Dairy Research Institute, Karnal 132001, India
    4、Department of Clinical Studies, School of Veterninary Medicine, University of Pennsylvania, Pennsylvania 19348, USA
    5、Microbial Science Division, Agharkar Research Institute, Pune 411004, India
  • 收稿日期:2013-10-21 出版日期:2015-03-01 发布日期:2015-03-12
  • 通讯作者: Anil Kumar Puniya, E-mail: akpuniya@gmail.com
  • 基金资助:

    The work was conducted as a part of a Ph D project of Sanjay Kumar that was supported by NDRI (ICAR) fellowship. The authors are also grateful to National Initiative on Climate Resilient Agriculture, India (NICRA) for providing partial support.

Prospective use of bacteriocinogenic Pediococcus pentosaceus as direct-fed microbial having methane reducing potential

 Sanjay Kumar, Sumit S Dagar, Seyed H Ebrahimi, Ravinder K Malik, Ramesh C Upadhyay, AnilK Puniya   

  1. 1、Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
    2、Dairy Cattle Nutrition, National Dairy Research Institute, Karnal 132001, India
    3、Dairy Cattle Physiology, National Dairy Research Institute, Karnal 132001, India
    4、Department of Clinical Studies, School of Veterninary Medicine, University of Pennsylvania, Pennsylvania 19348, USA
    5、Microbial Science Division, Agharkar Research Institute, Pune 411004, India
  • Received:2013-10-21 Online:2015-03-01 Published:2015-03-12
  • Contact: Anil Kumar Puniya, E-mail: akpuniya@gmail.com
  • Supported by:

    The work was conducted as a part of a Ph D project of Sanjay Kumar that was supported by NDRI (ICAR) fellowship. The authors are also grateful to National Initiative on Climate Resilient Agriculture, India (NICRA) for providing partial support.

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摘要： Direct-fed microbials (DFM), generally regarded as safe status, are successfully used in improving rumen ecology, gastro-intestinal health, feed efficiency, milk production and growth rate in ruminants. On the other hand, methanogenesis in rumen, which accounts for a significant loss of ruminant energy and increased greenhouse gas in environment, is of great concern, therefore, use of DFM for improving productivity without compromising the animal health and ecological sustainability is encouraged. The present study was conducted to investigate the methane reducing potential of bacteriocinogenic strain Pediococcus pentosaceus-34. Since, the culture showed no hemolysis on blood agar and DNase activity, hence, it was considered to be avirulent in nature, a prerequisite for any DFM. The culture also showed tolerance to pH 5.0 for 24 h with 0.5% organic acid mixture, whereas when given a shock for 2 h at different pH and organic acids concentrations, it showed growth at pH 3.0 and 4.0 with 0.1 and 1.0% organic acids, respectively, as having good animal probiotics attributes. The total gas production was significantly (P<0.05) higher in live pedicoccal culture (LPC) and dead pedicoccal culture (DPC) both with wheat straw, when compared to the control. In sugarcane bagasse, gas production was significantly lower (P<0.05) with LPC compared to the control and DPC both. Methane was reduced by the inclusion of LPC in sugarcane bagasse (0.07 mL CH4 mg–1 dry matter digestibility) with no effect on other rumen fermentation parameters. However, with wheat straw and LPC total gas, in vitro dry matter digestibility, total volatile fatty acids increased significantly but no reduction in methane production was observed in comparison to the control. Therefore, further research is warranted in this direction, if the bacteriocinogenic strains can be used as DFM for ruminants to improve the ruminant productivity.

关键词: Pediococcus pentosaceus , bacteriocin , methane , direct-fed microbials , rumen , probiotics

Abstract: Direct-fed microbials (DFM), generally regarded as safe status, are successfully used in improving rumen ecology, gastro-intestinal health, feed efficiency, milk production and growth rate in ruminants. On the other hand, methanogenesis in rumen, which accounts for a significant loss of ruminant energy and increased greenhouse gas in environment, is of great concern, therefore, use of DFM for improving productivity without compromising the animal health and ecological sustainability is encouraged. The present study was conducted to investigate the methane reducing potential of bacteriocinogenic strain Pediococcus pentosaceus-34. Since, the culture showed no hemolysis on blood agar and DNase activity, hence, it was considered to be avirulent in nature, a prerequisite for any DFM. The culture also showed tolerance to pH 5.0 for 24 h with 0.5% organic acid mixture, whereas when given a shock for 2 h at different pH and organic acids concentrations, it showed growth at pH 3.0 and 4.0 with 0.1 and 1.0% organic acids, respectively, as having good animal probiotics attributes. The total gas production was significantly (P<0.05) higher in live pedicoccal culture (LPC) and dead pedicoccal culture (DPC) both with wheat straw, when compared to the control. In sugarcane bagasse, gas production was significantly lower (P<0.05) with LPC compared to the control and DPC both. Methane was reduced by the inclusion of LPC in sugarcane bagasse (0.07 mL CH4 mg–1 dry matter digestibility) with no effect on other rumen fermentation parameters. However, with wheat straw and LPC total gas, in vitro dry matter digestibility, total volatile fatty acids increased significantly but no reduction in methane production was observed in comparison to the control. Therefore, further research is warranted in this direction, if the bacteriocinogenic strains can be used as DFM for ruminants to improve the ruminant productivity.

Key words: Pediococcus pentosaceus , bacteriocin , methane , direct-fed microbials , rumen , probiotics