Promotion and Inhibition of Ruminal Epithelium Growth by Butyric Acid and Insulin-Like Growth Factor-1 (IGF-1) in Dairy Goats

doi:10.1016/S2095-3119(13)60603-6

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (9): 2005-2009.DOI: 10.1016/S2095-3119(13)60603-6

Promotion and Inhibition of Ruminal Epithelium Growth by Butyric Acid and Insulin-Like Growth Factor-1 (IGF-1) in Dairy Goats

LIU Da-cheng, ZHOU Xiang-li, LIU Guo-juan, GAO Min , HU Hong-lian

1、College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China
2、Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, P.R.China
3、Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010030, P.R.China

收稿日期:2013-04-08 出版日期:2014-09-22 发布日期:2014-09-24
通讯作者: LIU Da-cheng, Mobile: 13664816212, E-mail: nmgldc@163.com
基金资助:
This work was supported by the National Natural Science Foundation of China (30960252) and the China Agriculture Research System (CARS-37).

Promotion and Inhibition of Ruminal Epithelium Growth by Butyric Acid and Insulin-Like Growth Factor-1 (IGF-1) in Dairy Goats

LIU Da-cheng, ZHOU Xiang-li, LIU Guo-juan, GAO Min , HU Hong-lian

1、College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China
2、Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Hohhot 010018, P.R.China
3、Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010030, P.R.China

Received:2013-04-08 Online:2014-09-22 Published:2014-09-24
Contact: LIU Da-cheng, Mobile: 13664816212, E-mail: nmgldc@163.com
Supported by:
This work was supported by the National Natural Science Foundation of China (30960252) and the China Agriculture Research System (CARS-37).

摘要/Abstract

摘要： Isolated ruminal epithelia from caudal blind sacs of dairy goats were incubated with butyrate and insulin-like growth factor-1 (IGF-1) at different concentrations. Proportions of ruminal epithelium in different phases of the cell division cycle were determined by flow cytometric analysis. The proportion of epithelial cells in S phase and G2-M phase (PS&G2-M) increased significantly (P<0.01) whereas the proportion of epithelial cells in G0-G1 phase (PG0-G1) decreased after incubation with IGF-1. PS&G2-M decreased whereas PG0-G1 increased markedly (P<0.01) after incubation with sodium butyrate. PS&G2-M incubated with IGF-1 and butyrate sodium together increased more than that incubated with IGF-1 alone; PG0-G1, however, decreased significantly (P<0.01). Our results indicate that IGF-1 enhances whereas sodium butyrate inhibits the proliferation of rumen epithelial cells. Furthermore, butyrate and IGF-1, together, have a synergic effect on the proliferation of rumen epithelium.

关键词: butyrate , IGF-1 , rumen epithelium

Abstract: Isolated ruminal epithelia from caudal blind sacs of dairy goats were incubated with butyrate and insulin-like growth factor-1 (IGF-1) at different concentrations. Proportions of ruminal epithelium in different phases of the cell division cycle were determined by flow cytometric analysis. The proportion of epithelial cells in S phase and G2-M phase (PS&G2-M) increased significantly (P<0.01) whereas the proportion of epithelial cells in G0-G1 phase (PG0-G1) decreased after incubation with IGF-1. PS&G2-M decreased whereas PG0-G1 increased markedly (P<0.01) after incubation with sodium butyrate. PS&G2-M incubated with IGF-1 and butyrate sodium together increased more than that incubated with IGF-1 alone; PG0-G1, however, decreased significantly (P<0.01). Our results indicate that IGF-1 enhances whereas sodium butyrate inhibits the proliferation of rumen epithelial cells. Furthermore, butyrate and IGF-1, together, have a synergic effect on the proliferation of rumen epithelium.

Key words: butyrate , IGF-1 , rumen epithelium

LIU Da-cheng, ZHOU Xiang-li, LIU Guo-juan, GAO Min , HU Hong-lian. Promotion and Inhibition of Ruminal Epithelium Growth by Butyric Acid and Insulin-Like Growth Factor-1 (IGF-1) in Dairy Goats[J]. Journal of Integrative Agriculture, 2014, 13(9): 2005-2009.

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Promotion and Inhibition of Ruminal Epithelium Growth by Butyric Acid and Insulin-Like Growth Factor-1 (IGF-1) in Dairy Goats

Promotion and Inhibition of Ruminal Epithelium Growth by Butyric Acid and Insulin-Like Growth Factor-1 (IGF-1) in Dairy Goats

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