Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1817-1826.DOI: 10.1016/S2095-3119(14)60754-1

• 论文 • 上一篇    下一篇

Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability

 GAO Xiao-guang, WANG Zhen-yu, TANG Meng-tian, MA Chang-wei , DAI Rui-tong   

  1. 1、College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R.China
    2、College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, P.R.China
    3、Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products
    Processing, Ministry of Agriculture, Beijing 100193, P.R.China
  • 收稿日期:2013-02-22 出版日期:2014-08-01 发布日期:2014-08-02
  • 通讯作者: DAI Rui-tong, Tel: +86-10-62737644, Mobile: 15376776069, Fax: +86-10-62839300, E-mail: dairuitong@hotmail.com
  • 作者简介:GAO Xiao-guang, E-mail: gaoxiaoguang999@126.com
  • 基金资助:

    the financial support by the National Natural Science Foundation of China (31071624).

Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability

 GAO Xiao-guang, WANG Zhen-yu, TANG Meng-tian, MA Chang-wei , DAI Rui-tong   

  1. 1、College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R.China
    2、College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, P.R.China
    3、Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products
    Processing, Ministry of Agriculture, Beijing 100193, P.R.China
  • Received:2013-02-22 Online:2014-08-01 Published:2014-08-02
  • Contact: DAI Rui-tong, Tel: +86-10-62737644, Mobile: 15376776069, Fax: +86-10-62839300, E-mail: dairuitong@hotmail.com
  • About author:GAO Xiao-guang, E-mail: gaoxiaoguang999@126.com
  • Supported by:

    the financial support by the National Natural Science Foundation of China (31071624).

PDF

摘要： In two experiments, the effects of succinate and NADH (reduced nicotinamide adenine dinucleotide) on metmyoglobin reductase activity and electron transport chain-linked metmyoglobin reduction were investigated and compared. In experiment 1, metmyoglobin (MetMb), substrate and inhibitors were incubated with mitochondria. Comparsion of the effects of succinate and NADH on MetMb reduction was investigated. The MetMb percentage in sample treated with 8 mol L-1 succinate decreased by about 69% after 3 h incubation, and the effect was inhibited by the addition of 10 mol L-1 electron transfer chain complex II inhibitor malonic acid; the MetMb percentage in samples treated with 2 mol L-1 NADH decreased by 56% and the effect was inhibited by the addition of 0.02 mol L-1 electron transport chain complex I inhibitor rotenone. These results indicated that electron transport chain played an important role in MetMb reduction. Both complex II and complex I take part in the MetMb reduction in mitochondria through different pathways. NADH-MetMb reduction system was less stable than succinate- MetMb system. In experiment 2, the beef longissimus dorsi muscle was blended with different concentrations of succinate or NADH. Enhancing patties with higher concentration of succinate or NADH improved colour stability in vacuum packaged samples (P<0.05). These results verified that mitochondria electron transport chain is related to the MetMb reduction in meat system.

关键词: succinate, NADH, malonic acid, rotenone, metmyoglobin, electron transport chain

Abstract: In two experiments, the effects of succinate and NADH (reduced nicotinamide adenine dinucleotide) on metmyoglobin reductase activity and electron transport chain-linked metmyoglobin reduction were investigated and compared. In experiment 1, metmyoglobin (MetMb), substrate and inhibitors were incubated with mitochondria. Comparsion of the effects of succinate and NADH on MetMb reduction was investigated. The MetMb percentage in sample treated with 8 mol L-1 succinate decreased by about 69% after 3 h incubation, and the effect was inhibited by the addition of 10 mol L-1 electron transfer chain complex II inhibitor malonic acid; the MetMb percentage in samples treated with 2 mol L-1 NADH decreased by 56% and the effect was inhibited by the addition of 0.02 mol L-1 electron transport chain complex I inhibitor rotenone. These results indicated that electron transport chain played an important role in MetMb reduction. Both complex II and complex I take part in the MetMb reduction in mitochondria through different pathways. NADH-MetMb reduction system was less stable than succinate- MetMb system. In experiment 2, the beef longissimus dorsi muscle was blended with different concentrations of succinate or NADH. Enhancing patties with higher concentration of succinate or NADH improved colour stability in vacuum packaged samples (P<0.05). These results verified that mitochondria electron transport chain is related to the MetMb reduction in meat system.