Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations

doi:10.1016/S2095-3119(13)60516-X

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (4): 819-826.DOI: 10.1016/S2095-3119(13)60516-X

Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations

ZHAO Xia, JIA Miao, WANG Lei, CAO Guang-chun , ZHANG Ze-hua

1、State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,
Beijing 100193, P.R.China
2、College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China

收稿日期:2013-02-27 出版日期:2014-04-01 发布日期:2014-04-16
通讯作者: CAO Guang-chun, Tel/Fax: +86-10-82109585, E-mail: caoguangchun77@163.com
基金资助:
This study was supported by the the Special Fund for Agro-Scientific Research in the Public Interest of China (201003079) and the Earmarked Fund for China Agriculture Research System (CARS-35-07).

Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations

ZHAO Xia, JIA Miao, WANG Lei, CAO Guang-chun , ZHANG Ze-hua

1、State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,
Beijing 100193, P.R.China
2、College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, P.R.China

Received:2013-02-27 Online:2014-04-01 Published:2014-04-16
Contact: CAO Guang-chun, Tel/Fax: +86-10-82109585, E-mail: caoguangchun77@163.com
Supported by:
This study was supported by the the Special Fund for Agro-Scientific Research in the Public Interest of China (201003079) and the Earmarked Fund for China Agriculture Research System (CARS-35-07).

摘要/Abstract

摘要： Rapid and mass rearing of Locusta migratoria manilensis is an urgent need to meet the increasing demand for food of people. In this study, the effects of four artificial feeds on the development, reproduction and the activities of detoxification and protective enzymes of L. migratoria manilensis in three successive generations were investigated. The results showed that sucrose and monosodium glutamate (MSG) significantly increased the net reproductive rate (R0) and the intrinsic growth rate (rm) of L. migratoria manilensis, but sodium chloride (0.17%) suppressed this increase. Furthermore, the artificial feed with sucrose and monosodium glutamate increased the activities of esterase (EST), acetylcholinesterase (AChE), glutathione-Stransferase (GST), multi-function oxidase (MFO), phenol oxidase (PO), catalase (CAT) and peroxidase (POD), but inhibited the activity of superoxide dismutase (SOD). However, sodium chloride (0.17%) increased the activities of EST, AChE, CAT and SOD, and inhibited the activities of MFO, GST, PO and POD. Correlation analysis found that the increasing of PO activity and the decreasing of SOD activities were significantly related with the increasing of the intrinsic growth rate (rm). The above results indicated that sucrose and monosodium glutamate could promote the development and reproduction of L. migratoria manilensis, but Na+ inhibit such promotion with the concentration above 0.2%. The activities of PO and SOD can be used as biochemical standard to assess the effect of artificial feed.

关键词: Locusta migratoria manilensis , artificial feed , intrinsic rate , phenol oxidase , superoxide dismutase

Abstract: Rapid and mass rearing of Locusta migratoria manilensis is an urgent need to meet the increasing demand for food of people. In this study, the effects of four artificial feeds on the development, reproduction and the activities of detoxification and protective enzymes of L. migratoria manilensis in three successive generations were investigated. The results showed that sucrose and monosodium glutamate (MSG) significantly increased the net reproductive rate (R0) and the intrinsic growth rate (rm) of L. migratoria manilensis, but sodium chloride (0.17%) suppressed this increase. Furthermore, the artificial feed with sucrose and monosodium glutamate increased the activities of esterase (EST), acetylcholinesterase (AChE), glutathione-Stransferase (GST), multi-function oxidase (MFO), phenol oxidase (PO), catalase (CAT) and peroxidase (POD), but inhibited the activity of superoxide dismutase (SOD). However, sodium chloride (0.17%) increased the activities of EST, AChE, CAT and SOD, and inhibited the activities of MFO, GST, PO and POD. Correlation analysis found that the increasing of PO activity and the decreasing of SOD activities were significantly related with the increasing of the intrinsic growth rate (rm). The above results indicated that sucrose and monosodium glutamate could promote the development and reproduction of L. migratoria manilensis, but Na+ inhibit such promotion with the concentration above 0.2%. The activities of PO and SOD can be used as biochemical standard to assess the effect of artificial feed.

Key words: Locusta migratoria manilensis , artificial feed , intrinsic rate , phenol oxidase , superoxide dismutase

ZHAO Xia, JIA Miao, WANG Lei, CAO Guang-chun , ZHANG Ze-hua. Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations[J]. Journal of Integrative Agriculture, 2014, 13(4): 819-826.

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Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations

Effects of Glutamate and Na+ on the Development and Enzyme Activity of the Oriental Migratory Locust, Locusta migratoria manilensis (Meyen) in Successive Generations

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