RESEARCH ARTICLE Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks

doi:10.1016/S1671-2927(00)8644

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (7): 1173-1180.DOI: 10.1016/S1671-2927(00)8644

RESEARCH ARTICLE Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks

Khondowe Paul, WANG Song-bo, CHEN Sheng-feng, YU Jian-jian, ZHU Xiao-tong, WANG Li-na, GAO Ping, XI Qian-yun, ZHANG Yong-liang, SHU Gang, JIANG Qing-yan

1.College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R.China
2.School of Natural Sciences, Department of Biological Sciences, University of Zambia, P.O. Box 32379, Lusaka, Zambia

收稿日期:2011-03-29 出版日期:2012-07-01 发布日期:2012-07-27
通讯作者: JIANG Qing-yan, Tel: +86-20-85284930, Fax: +86-20-85284901, E-mail: qyjiang@scau.edu.cn; SHU Gang, Tel: +86-20-85284901, Fax: +86-20- 85284901, E-mail: shugang@scau.edu.cn
基金资助:
This work was supported by the National Basic Research Program of China (2009CB941601), the Joint Funds of the National Natural Science Foundation of China (u0731004), the National Natural Science Foundation of China (30871845, 30901058 and 30972157), the Natural Science Foundation of Guangdong Province of China (9451064201003790 and 9151064201000056), the National Public Benefit (Agricultural) Research Foundation of China (201003011), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20094404120012).

RESEARCH ARTICLE Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks

Khondowe Paul, WANG Song-bo, CHEN Sheng-feng, YU Jian-jian, ZHU Xiao-tong, WANG Li-na, GAO Ping, XI Qian-yun, ZHANG Yong-liang, SHU Gang, JIANG Qing-yan

1.College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R.China
2.School of Natural Sciences, Department of Biological Sciences, University of Zambia, P.O. Box 32379, Lusaka, Zambia

Received:2011-03-29 Online:2012-07-01 Published:2012-07-27
Contact: JIANG Qing-yan, Tel: +86-20-85284930, Fax: +86-20-85284901, E-mail: qyjiang@scau.edu.cn; SHU Gang, Tel: +86-20-85284901, Fax: +86-20- 85284901, E-mail: shugang@scau.edu.cn
Supported by:
This work was supported by the National Basic Research Program of China (2009CB941601), the Joint Funds of the National Natural Science Foundation of China (u0731004), the National Natural Science Foundation of China (30871845, 30901058 and 30972157), the Natural Science Foundation of Guangdong Province of China (9451064201003790 and 9151064201000056), the National Public Benefit (Agricultural) Research Foundation of China (201003011), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20094404120012).

摘要/Abstract

摘要： Different amino acids have been shown to affect feed intake when injected directly into the central nervous system of birds. In the present study, we investigated the effects of L-glutamine and L-alanine on feed intake and the mRNA expression levels of hypothalamic neuropeptides involved in feed intake regulation in broiler chicks. L-Glutamine or Lalanine was intra-cerebroventricularly (ICV) administered to 4-d-old broiler chicks and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of neuropeptide Y (NPY), agouti related protein (AgRP), pro-opiomelanocortin (POMC), melanocortin receptor 4 (MC4R) and corticotropin releasing factor (CRF). Our results showed that ICV administration of L-glutamine (0.55 or 5.5 μmol) significantly increased feed intake up to 2 h post-administration period and the hypothalamic NPY mRNA expression levels, while it markedly decreased hypothalamic POMC and CRF mRNA expression levels. In contrast, ICV administration of L-alanine (4 μmol) significantly decreased feed intake for the first 0.5 h post-administration period, and reduced the hypothalamic AgRP mRNA expression levels, while it remarkablely enhanced the mRNA expression levels of MC4R and CRF. These findings suggested that L-glutamine and L-alanine could act within the hypothalamus to influence feed intake in broiler chicks, and that both orexigenic and anorexigenic neuropeptide genes might contribute directly to these effects.

关键词: L-glutamine, L-alanine, intracerebroventricular (ICV), feed intake, hypothalamus

Abstract: Different amino acids have been shown to affect feed intake when injected directly into the central nervous system of birds. In the present study, we investigated the effects of L-glutamine and L-alanine on feed intake and the mRNA expression levels of hypothalamic neuropeptides involved in feed intake regulation in broiler chicks. L-Glutamine or Lalanine was intra-cerebroventricularly (ICV) administered to 4-d-old broiler chicks and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of neuropeptide Y (NPY), agouti related protein (AgRP), pro-opiomelanocortin (POMC), melanocortin receptor 4 (MC4R) and corticotropin releasing factor (CRF). Our results showed that ICV administration of L-glutamine (0.55 or 5.5 μmol) significantly increased feed intake up to 2 h post-administration period and the hypothalamic NPY mRNA expression levels, while it markedly decreased hypothalamic POMC and CRF mRNA expression levels. In contrast, ICV administration of L-alanine (4 μmol) significantly decreased feed intake for the first 0.5 h post-administration period, and reduced the hypothalamic AgRP mRNA expression levels, while it remarkablely enhanced the mRNA expression levels of MC4R and CRF. These findings suggested that L-glutamine and L-alanine could act within the hypothalamus to influence feed intake in broiler chicks, and that both orexigenic and anorexigenic neuropeptide genes might contribute directly to these effects.

Key words: L-glutamine, L-alanine, intracerebroventricular (ICV), feed intake, hypothalamus

Khondowe Paul, WANG Song-bo, CHEN Sheng-feng, YU Jian-jian, ZHU Xiao-tong, WANG Li-na, GAO Ping, XI Qian-yun, ZHANG Yong-liang, SHU Gang, JIANG Qing-yan . RESEARCH ARTICLE Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks[J]. Journal of Integrative Agriculture, 2012, 12(7): 1173-1180.

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RESEARCH ARTICLE Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks

RESEARCH ARTICLE Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks

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