Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (2): 358-364.DOI: 10.1016/S2095-3119(13)60373-1

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Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase (SPS) Activity in Two Tomato Species

 WANG Li, CUI Na, ZHAO Xiao-cui, FAN Hai-yan , LI Tian-lai   

  1. 1.Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, P.R.China
    2.Key Laboratory of Protected Horticulture, Ministry of Education/College of Horticulture, Shenyang Agricultural University, Shenyang 110866,
    P.R.China
  • 收稿日期:2012-12-10 出版日期:2014-02-01 发布日期:2014-02-06
  • 通讯作者: CUI Na, Tel: +86-24-88487163, E-mail: syaua@163.com
  • 作者简介:WANG Li, E-mail: sdwl881209@yahoo.com.cn
  • 基金资助:

    This study was supported by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD12B03).

Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase (SPS) Activity in Two Tomato Species

 WANG Li, CUI Na, ZHAO Xiao-cui, FAN Hai-yan , LI Tian-lai   

  1. 1.Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, P.R.China
    2.Key Laboratory of Protected Horticulture, Ministry of Education/College of Horticulture, Shenyang Agricultural University, Shenyang 110866,
    P.R.China
  • Received:2012-12-10 Online:2014-02-01 Published:2014-02-06
  • Contact: CUI Na, Tel: +86-24-88487163, E-mail: syaua@163.com
  • About author:WANG Li, E-mail: sdwl881209@yahoo.com.cn
  • Supported by:

    This study was supported by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD12B03).

摘要: To explore the differences of carbohydrate metabolism in two tomato species and discuss the possible regulation of 14-3-3 proteins on the sucrose phosphate synthase (SPS) activity, we determined the contents of soluble sugar and starch through high performance liquid chromatography (HPLC). The activities of sugar-metabolizing enzymes were assayed in desalted extract, and the relative expression levels of related genes in sugar metabolism were determined though real-time RT-PCR. The results indicated that glucose and fructose were mainly accumulated during the maturation of the fruit because of the high acid invertase (AI) and neutral invertase (NI) in Micro-Tom (Solanum lycopersicum) fruit, while in Solanum chmielewskii fruit, SPS which went along with the change of sucrose content led to the rapid sucrose increase during the fruit ripening. TFT1 and TFT10, belonging to 14-3-3 protein in tomato, were likely to down-regulated SPS activity during young and intumescence period.

关键词: Micro-Tom , Solanum chmielewskii , carbohydrate accumulation , 14-3-3 proteins , fruit development

Abstract: To explore the differences of carbohydrate metabolism in two tomato species and discuss the possible regulation of 14-3-3 proteins on the sucrose phosphate synthase (SPS) activity, we determined the contents of soluble sugar and starch through high performance liquid chromatography (HPLC). The activities of sugar-metabolizing enzymes were assayed in desalted extract, and the relative expression levels of related genes in sugar metabolism were determined though real-time RT-PCR. The results indicated that glucose and fructose were mainly accumulated during the maturation of the fruit because of the high acid invertase (AI) and neutral invertase (NI) in Micro-Tom (Solanum lycopersicum) fruit, while in Solanum chmielewskii fruit, SPS which went along with the change of sucrose content led to the rapid sucrose increase during the fruit ripening. TFT1 and TFT10, belonging to 14-3-3 protein in tomato, were likely to down-regulated SPS activity during young and intumescence period.

Key words: Micro-Tom , Solanum chmielewskii , carbohydrate accumulation , 14-3-3 proteins , fruit development