Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis

doi:10.1016/S2095-3119(13)60220-8

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (2): 209-217.DOI: 10.1016/S2095-3119(13)60220-8

Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis

LI Jing, SONG Su-sheng, ZHAO Yu-sheng, GUO Wei-wei, GUO Guang-hui, PENG Hui-ru, NI Zhong-fu

1.State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization, Minstry of Education/Key Laboratory
of Crop Genomics and Genetic Improvement, Minstry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
2.School of Life Sciences, Tsinghua University, Beijing 100084, P.R.China

收稿日期:2012-04-15 出版日期:2013-02-01 发布日期:2013-02-06
通讯作者: Correspondence YAO Ying-yin, Tel/Fax: +86-10-62731452, E-mail: yingyin@cau.edu.cn
基金资助:
This work was financially supported by the National Natural Science Foundation of China (30600392, 30871529), the National “863” Program of China (2012AA10A309).

Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis

LI Jing, SONG Su-sheng, ZHAO Yu-sheng, GUO Wei-wei, GUO Guang-hui, PENG Hui-ru, NI Zhong-fu

1.State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization, Minstry of Education/Key Laboratory
of Crop Genomics and Genetic Improvement, Minstry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
2.School of Life Sciences, Tsinghua University, Beijing 100084, P.R.China

Received:2012-04-15 Online:2013-02-01 Published:2013-02-06
Contact: Correspondence YAO Ying-yin, Tel/Fax: +86-10-62731452, E-mail: yingyin@cau.edu.cn
Supported by:
This work was financially supported by the National Natural Science Foundation of China (30600392, 30871529), the National “863” Program of China (2012AA10A309).

摘要/Abstract

摘要： 14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat (Triticum aestivum L., 2n=6x=42, AABBDD) and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.

关键词: wheat , Ta14-3-3 , heterosis , gene expression

Abstract: 14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat (Triticum aestivum L., 2n=6x=42, AABBDD) and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.

Key words: wheat , Ta14-3-3 , heterosis , gene expression

LI Jing, SONG Su-sheng, ZHAO Yu-sheng, GUO Wei-wei, GUO Guang-hui, PENG Hui-ru, NI Zhong-fu. Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis[J]. Journal of Integrative Agriculture, 2013, 12(2): 209-217.

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Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis

Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis

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