Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (12): 1986-1991.DOI: 10.1016/S1671-2927(11)60200-9

• 论文 • 上一篇    下一篇

Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-Inositol, Vitexin, and Isovitexin Contents in Mung Bean

 YAO Yang, CHENG Xu-zhen , REN Gui-xing   

  1. 1.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • 收稿日期:2010-09-29 出版日期:2011-12-01 发布日期:2011-12-19
  • 通讯作者: Correspondence REN Gui-xing, Tel: +86-10-62115596, Fax: +86-10-62156596, E-mail: renguixing@caas.net.cn
  • 基金资助:

    The present study was supported by the Talent Fund (to Dr. Ren Guixing) from the Chinese Academy of Agricultural Sciences and the Earmarked Fund for Modern Agro-Industry Technology Research System, China (nycytx-018).

Application of Near-Infrared Reflectance Spectroscopy to the Evaluation of D-chiro-Inositol, Vitexin, and Isovitexin Contents in Mung Bean

 YAO Yang, CHENG Xu-zhen , REN Gui-xing   

  1. 1.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • Received:2010-09-29 Online:2011-12-01 Published:2011-12-19
  • Contact: Correspondence REN Gui-xing, Tel: +86-10-62115596, Fax: +86-10-62156596, E-mail: renguixing@caas.net.cn
  • Supported by:

    The present study was supported by the Talent Fund (to Dr. Ren Guixing) from the Chinese Academy of Agricultural Sciences and the Earmarked Fund for Modern Agro-Industry Technology Research System, China (nycytx-018).

摘要: Mung bean (Vigna radiata L.) is rich in D-chiro-inositol (DCI), vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy (NIRS) was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography (HPLC). The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares (PLS) algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin). The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.

关键词: D-chiro-inositol, vitexin, isovitexin, NIRS, mung bean

Abstract: Mung bean (Vigna radiata L.) is rich in D-chiro-inositol (DCI), vitexin, and isovitexin, which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products. In this study, near-infrared reflectance spectroscopy (NIRS) was used to predict the contents of DCI, vitexin, and isovitexin in mung bean. The spectra data were linearized with those determined by high-performance liquid chromatography (HPLC). The models for predicting the DCI, vitexin, and isovitexin contents in mung bean were developed using partial least-squares (PLS) algorithm. Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (R2=0.90 for DCI, R2=0.81 for vitexin, and R2=0.90 for isovitexin). The predictive contents of DCI, vitexin, and isovitexin ranged from 2.082 to 3.084%, 1.277 to 1.307%, and 0.5998 to 0.6286%, respectively. The results showed that NIRS, a well-established and widely applied technique, could be applied to rapid detection of DCI, vitexin, and isovitexin contents in mung bean.

Key words: D-chiro-inositol, vitexin, isovitexin, NIRS, mung bean