Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage

doi:10.1016/S1671-2927(11)60164-8

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (11): 1653-1664.DOI: 10.1016/S1671-2927(11)60164-8

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Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage

HU Biao-lin, FU Xue-qin, ZHANG Tao, WAN Yong, LI Xia, HUANG Yun-hong, DAI Liang-fang, LUO Xiang-dong , XIE Jian-kun

1.Rice Research Institute, Jiangxi Academy of Agricultural Sciences
2.College of Life Sciences, Jiangxi Normal University

收稿日期:2010-11-09 出版日期:2011-11-01 发布日期:2011-11-07
通讯作者: Correspondence XIE Jian-kun, Professor, Mobile: 13707082911, E-mail: xiejiankun@yahoo.com
作者简介:HU Biao-lin, MSc, Mobile: 13517911207, E-mail: hubiaolin992@yahoo.com.cn
基金资助:
This project was finiancially supported by the National Natural Science Fundation of China (30960189), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China, the project for Principle Research Topic of Jiangxi Education, China (GJJ08146), the Jiangxi Province Project for Principle Research Leader, China (020007), the Jiangxi Province Inviting Tender Project for Principle Research Topic, China (20068), the Natural Science Foundation of Jiangxi Province, China (2009GQN0068), and the Youth Foundation of Jiangxi Academy of Agricultural Sciences, China (2010CQN008).

Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage

HU Biao-lin, FU Xue-qin, ZHANG Tao, WAN Yong, LI Xia, HUANG Yun-hong, DAI Liang-fang, LUO Xiang-dong , XIE Jian-kun

1.Rice Research Institute, Jiangxi Academy of Agricultural Sciences
2.College of Life Sciences, Jiangxi Normal University

Received:2010-11-09 Online:2011-11-01 Published:2011-11-07
Contact: Correspondence XIE Jian-kun, Professor, Mobile: 13707082911, E-mail: xiejiankun@yahoo.com
About author:HU Biao-lin, MSc, Mobile: 13517911207, E-mail: hubiaolin992@yahoo.com.cn
Supported by:
This project was finiancially supported by the National Natural Science Fundation of China (30960189), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China, the project for Principle Research Topic of Jiangxi Education, China (GJJ08146), the Jiangxi Province Project for Principle Research Leader, China (020007), the Jiangxi Province Inviting Tender Project for Principle Research Topic, China (20068), the Natural Science Foundation of Jiangxi Province, China (2009GQN0068), and the Youth Foundation of Jiangxi Academy of Agricultural Sciences, China (2010CQN008).

摘要/Abstract

摘要： Drought stress is one of the major constraints to rice (Oryza sativa L.) production and yield stability especially in rainfed ecosystems and is getting worse as the climate changes worldwide. Dongxiang wild rice (DXWR) Oryza rufipogon Griff., contains drought resistant gene. Improving drought resistance of cultivars is crucial to increase and stabilize rice grain yield via transferring resistant gene from species related to rice. In this paper, four upland rice, sixty backcross inbred lines (BILs) derived from BC1F5 of R974//DXWR/R974, and their parents were employed to evaluate drought-resistance at seedling stage in the greenhouse. Nine traits were recorded for assessment of drought resistance, including maximum root length (MRL), number of roots (NR), shoot length (SL), dry root weight (DRW), fresh root weight (FRW), root relative water content (RRWC), leaf relative water content (LRWC), level for rolling leaf (LRL), and seedling survivability under repeat drought (SSRD). Using more than 88% of accumulative contribution resulted from the principal component analysis (PCA), the nine traits were classified into five independent principal components and the line 1949 showed the highest resistance. Analysis on the stepwise regression equation and correlation demonstrated that MRL, RN, FRW, and RRWC significantly influenced the drought resistance, thus could be used as comprehensive index for drought resistance at the seedling stage. Using the major gene plus polygene mixed inheritance model of quantitative traits, the inheritance of drought-resistance of BIL population at seedling stage was mostly controlled by two independent genes plus polygene. As a result, the DXWR could be precious resources for genetic improvement of drought resistance in cultivated rice.

关键词: Dongxiang wild rice (DXWR), drought resistance, principal component analysis (PCA), drought comprehensive index, seedling stage

Abstract: Drought stress is one of the major constraints to rice (Oryza sativa L.) production and yield stability especially in rainfed ecosystems and is getting worse as the climate changes worldwide. Dongxiang wild rice (DXWR) Oryza rufipogon Griff., contains drought resistant gene. Improving drought resistance of cultivars is crucial to increase and stabilize rice grain yield via transferring resistant gene from species related to rice. In this paper, four upland rice, sixty backcross inbred lines (BILs) derived from BC1F5 of R974//DXWR/R974, and their parents were employed to evaluate drought-resistance at seedling stage in the greenhouse. Nine traits were recorded for assessment of drought resistance, including maximum root length (MRL), number of roots (NR), shoot length (SL), dry root weight (DRW), fresh root weight (FRW), root relative water content (RRWC), leaf relative water content (LRWC), level for rolling leaf (LRL), and seedling survivability under repeat drought (SSRD). Using more than 88% of accumulative contribution resulted from the principal component analysis (PCA), the nine traits were classified into five independent principal components and the line 1949 showed the highest resistance. Analysis on the stepwise regression equation and correlation demonstrated that MRL, RN, FRW, and RRWC significantly influenced the drought resistance, thus could be used as comprehensive index for drought resistance at the seedling stage. Using the major gene plus polygene mixed inheritance model of quantitative traits, the inheritance of drought-resistance of BIL population at seedling stage was mostly controlled by two independent genes plus polygene. As a result, the DXWR could be precious resources for genetic improvement of drought resistance in cultivated rice.

Key words: Dongxiang wild rice (DXWR), drought resistance, principal component analysis (PCA), drought comprehensive index, seedling stage

HU Biao-lin, FU Xue-qin, ZHANG Tao, WAN Yong, LI Xia, HUANG Yun-hong, DAI Liang-fang, LUO Xiang-dong , XIE Jian-kun. Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage [J]. Journal of Integrative Agriculture, 2011, 10(11): 1653-1664.

参考文献

[1]Ahmad S, Ahmad R, Ashraf M Y, Ashraf M, Waraich A E. 2009. Sunflower (Helianthus annuus L.) response to drought stress at germination and seedling growth stages. Pakistan Journal of Botany, 41, 647-654.

[2]Ali A J, Xu J L, Ismail A M, Fu B Y, Vijaykumar C H M, Gao Y M, Domingo J, Maghirang R, Yu S B, Gregorio G, et al. 2006. Hidden diversity for abiotic and biotic stress tolerances in the primary gene pool of rice revealed by a large backcross breeding program. Field Crops Research, 97, 66-76.

[3]Ali M A, Abbas A, Niaz S, Zulkkfal M, Ali S. 2009. Morphophysiological criteria for drought tolerance in sorghum (Sorghum bicolor) at seedling and post-anthesis stages. International Journal of Agriculture and Biology, 11, 674-680.

[4]Aline R R, Cléber M G, Paulo H N R, Felipe R da S, Daniela S, Emanuel de S, Ana C M B, Carlos R S, Márcio E F, Ângela M. 2008. Identification of drought-responsive genes in roots of upland rice (Oryza sativa L.). BMC Genomics, 9, 485-497.

[5]Bay D N, Darshan S B, Buu C B, Nguyen T V, Pham L N, Nguyen H T. 2003. Identification and mapping of the QTL for aluminum tolerance introgressed from the new source, Oryza rufipogon Griff. into indica rice (Oryza sativa L.). Theoretical and Applied Genetics, 106, 583-593.

[6]Chen D Z, Zhong P A, Xiao Y Q, Huang,Y J, Xie J K. 2002. Identification of QTLs for cold tolerance at seedling stage in Dongxiang wild rice (Oryza rufipogon Griff.) by SSR markers. Acta Agriculturae Universitatis Jiangxiensis, 24, 754-756 . (in Chinese)

[7]Chen F M, Cheng J F, Pan X Y, Liu Y B. 2000. Screening for drought resistance traits and breeding utilization in indica rice. Acta Agricultuae Universitatis Jiangxiensis, 22, 169-173. (in Chinese)

[8]Chien H P. 2005. Rice consumption in China: Can China change rice consumption from quantity to quality? In: Toriyama K, Heong K L, Hardy B, eds., Rice Is Life: Scientific Perspectives for the 21st Century: Proceedings of the World Rice Research Conference. Tokyo and Tsukuba, Japan, 2004. International Rice Research Institute, Philippines. pp. 497-499.

[9]Farooq M, Wahid A, Lee D J, Ito O, Siddique K H M. 2009. Advances in drought resistance of rice. Critical Reviews in Plant Science, 28, 199-217.

[10]Gai J Y. 2005. Segregation analysis of genetic system of quantitative traits in plants. Hereditas, 27, 130-136. (in Chinese)

[11]Girma G, Tesfaye K, Bekele E. 2010. Inter simple sequence repeat (ISSR) analysis of wild and cultivated rice species from Ethiopia. African Journal of Biotechnology, 9, 5048-5059.

[12]Gu K, Tian D, Yang F, Wu L, Sreekala C, Wang D, Wang G L, Yin Z. 2004. High-resolution genetic mapping of Xa27(t), a new bacterial blight resistance gene in rice, Oryza sativa L. Theoretical and Applied Genetics, 108, 800-807.

[13]Guo L B, Qian Q. 2003. The methods of evaluating the drought resistance of cultivated rice varieties in field. China Rice, 2, 26-27. (in Chinese)

[14]Hameed A, Goher M, Iqbal N. 2010. Evaluation of seedling survivability and growth response as selection criteria for breeding drought tolerance in wheat. Cereal Research Communications, 38, 193-202.

[15]He G M, Luo X J, Tian F, Li K G, Zhu Z F, Su W, Qian X Y, Fu Y C, Wang X K, Sun C Q, Yang J S. 2006. Haplotype variation in structure and expression of a gene cluster associated with a quantitative trait locus for improved yield in rice. Genome Research, 16, 618-626.

[16]He X H, Gai J Y. 2006. Segregation analysis of quantitative traits in backcross inbred line population. Acta Agronomica Sinica, 32, 210-216. (in Chinese)

[17]Hu B L, Yu S W, Wan Y, Zhang Z, Qiu B Y, Xie J K. 2007. Drought-resistance identification of Dongxiang common wild rice (Oryza rufipogon Griff.) in whole growth period. Acta Agronomica Sinica, 33, 426-433. (in Chinese)

[18]Kato Y, Hirotsu S, Nemoto K, Yamagishi J. 2008. Identification of QTLs controlling rice drought tolerance at seedling stage in hydroponic culture. Euphytica, 260, 423-430.

[19]Lan Y Z, Hu J, Yang J C, Zhang Z J, Zhu Q S. 2003. Morphological and anatomic traits in drought-resistant rice root. Journal of Yanzhou University (Agricultural and Life Science Edition), 24, 58-61. (in Chinese)

[20]Li J B, Xia M Y, Qi H X, He G C, Wan B L, Zha Z P. 2006. Marker-assisted selection for brown planthopper (Nilaparvata lugens Stål) resistance genes Bph14 and Bph15 in rice. Scientia Agricultura Sinica, 39, 2132-2137. (in Chinese)

[21]Li Y, Ma J, Wang H Z, Zhang R P, Li X Y. 2005. Studies on screening of the drought resistance assessment indexes and comprehensive evaluation of rice varieties during seedling stage. Southwest China Journal of Agricultural Science, 18, 250-255. (in Chinese)

[22]Liu Y, Gai J Y, Lv H N, Wang Y J, Chen S Y. 2005. Identification of drought tolerant germplasm and inheritance and QTL mapping of related root traits in Soybean (Glycine max (L.) Merr.). Acta Genetica Sinica, 32, 855-863. (in Chinese)

[23]Lu G, Cai Q S, Liang Y M, Li K N, Liang Y T.1998. Preliminary report of identification on drought resistance of germplasm resource in rice at seedling stage. Guangxi Agricultural Sciences, 4, 166-167. (in Chinese)

[24]Luo L J, Zhang Q F. 2001. The status and strategy on drought resistance of rice (Oryza Sativa L.). Chinese Journal of Rice Science, 15, 209-214. (in Chinese)

[25]Mitra J. 2001. Genetics and genetic improvement of drought resistance in crop plants. Current Science, 80, 758-763.

[26]Rahman M L, Jiang W Z, Chu S H, Qiao Y L, Ham T H, Woo M O, Lee J Y, Khanam M S, Chin J H, Jeung J U, et al. 2009. High-resolution mapping of two rice brown planthopper resistance genes, Bph 20(t) and Bph 21(t), originating from Oryza minuta. Theoretical and Applied Genetics, 119, 1127-1246.

[27]Sakai H, Itoh T. 2010. Massive gene losses in Asian cultivated rice unveiled by comparative genome analysis. BMC Genomics, 11, 121-133.

[28]Serraj R, Hash C T, Buhariwalla K H, Bidinger R F, Folkertsma T R, Chandra S, Gaur M P, Kashiwagi J, Nigam N S, Rupakula R, et al. 2005. Marker-assisted breeding for crop drought tolerance at ICRISAT: Achievements and prospects. In: Tuberosa R, Phillips R L, Gale M, eds., In the Wake of the Double Helix: From the Green Revolution to the Gene Revolution. Proceedings of the International Congress, University of Bologna, Italy. pp. 217-238.

[29]Shlens J. 2005. A Tutorial on Principal Component Analysis. Systems Neurobiology Laboratory, Salk Institute for Biological Studies. Tang Q Y, Feng M G. 2002. DPS Data Processing System for Practical Statistics. Science Press, Beijing. pp. 363-373, 333-339. (in Chinese)

[30]Tuberosa R, Salvi S. 2006. Genomics-based approaches to improve drought tolerance of crops. Trends in Plant Science, 11, 405-412.

[31]Wang Y H, Yao Y Q, Zhang C J, Lu J J, Zhang J, Wang C H, Li J H. 2005. Study on drought-resistance identification methods and evaluation index of dry-land rice-evaluation of droughtresistance of dry-land rice in seedling stage. Agricultural Research in the Arid Areas, 23, 134-137. (in Chinese)

[32]Witcombe J R, Hollington P A, Howarth C J, Reader S, Steele K A. 2008. Breeding for abiotic stresses for sustainable agriculture. Philosophical Transactions of the Royal Society B, 363, 703-716.

[33]Xia R X, Xiao N, Hong Y H, Zhang C, Su Y, Zhang X M, Chen J M. 2010. QTLs mapping for cold tolerance at seedling stage in Dongxiang wild rice (Oryza rufipogon Griff.). Scientia Agricultura Sinica, 43, 443-451. (in Chinese)

[34]Xiao X C, Wang Y J, Xiao S J, Xiong X Y, Zhou J H, Zhou C Y. 2001. Breeding of new cytoplasmic male sterile line “Dong B11A” from Dongxiang wild rice. Acta Agriculturae Jiangxi, 13, 8-11. (in Chinese)

[35]Xie J K, Agrama H, Kong D L, Zhuang J Y, Hu B L, Wan Y, Yan W G. 2010a. Genetic diversity associated with conservation of endangered Dongxiang wild rice (Oryza rufipogon). Genetics Resource and Crop Evolution, 57, 597-609.

[36]Xie J K, Kong X L, Bao J S, Wan Y. 2004. Recent advances in molecular mapping and cloning of useful genes from wild rice and their application in breeding. Hereditas, 26, 115-121. (in Chinese)

[37]Xie J K, Hu B L, Wan Y, Zhang T, Li X, Liu R L, Huang Y H, Dai F L, Luo X D. 2010b. Comparison of the drought resistance characters at seedling stage between Dongxiang common wild rice (Oryza rufipogon Griff.) and cultivars (Oryza sativa L.). Acta Ecologica Sinica, 30, 1665-1674. (in Chinese)

[38]Xie J K, Wu X L, Jin L, Wan Y, Huang Y J, Bao J S. 2006. Identification of simple sequence repeats (SSR) markers for acid detergent fiber in rice straw by bulked segregant analysis. Journal of Agricultural and Food Chemistry, 54, 7616-7620.

[39]Yang H Y, Ren X, Weng Q M, Zhu L L, He G C. 2002. Molecular mapping and genetic analysis of a rice brown planthopper (Nilaparvata lugens Stal.) resistance gene. Hereditas, 136, 39-43.

[40]Ying C S. 1993a. Chinese Rice Germplasm Resource. Chinese Agricultural Science and Technology Press, Beijing. pp. 356-359. (in Chinese)

[41]Ying C S. 1993b. Chinese Rice Germplasm Resource. Chinese Agricultural Science and Technology Press, Beijing. pp. 540-542. (in Chinese)

[42]Yu L Q, Xu Q L, Qiu B Y, Xiong Y Z, Rao S F. 2007. Comparative studies on the main agronomic characteristics between in situ and ex situ conserved wild rice populations in Dongxiang. Journal of Plant Genetic Resources, 8, 99-101. (in Chinese)

[43]Yu S W, Wan Y, Hu B L, Zhang Z, Xie J K. 2005. The inheritance of the fertility restoration for cytoplasmic male sterility in Dongxiang wild rice (Oryza rufipogon). Molecular Plant Breeding, 3, 761-767. (in Chinese)

[44]Zhang J, Liu S M, Su Q P, Chen W Y, Jiang S X, Duan W Y. 2000. Preliminary study on yield potential and high-yielding cultivation of Brazil upland rice (CV. IAPAR9). Jiangsu Agricultural Research, 21, 44-46. (in Chinese)

[45]Zhang Z M, Wang S B, Dai L X, Song W W, Chen J, Shi Y Q. 2011. Estimating and screening of drought resistance indexes of peanut. Chinese Journal of Plant Ecology, 35, 100-109. (in Chinese)

[46]Zheng B S, Yang L, Zhang W P, Mao C Z, Wu Y R, Yi K K, Liu F Y, Wu P. 2003. Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations. Theoretical and Applied Genetics, 107, 1505-1515.

[47]Zhou G S, Cui K H, Jin D M, Cao C G, Xu C G, Luo B S. 2005. Studies on using rooting capacity to appraise the drought resistance of different varieties of Oryza sativa at seedling stage. Scientia Agricultura Sinica, 38, 2571-2576. (in Chinese)

[48]Zhou S X, Tian F, Zhu Z F, Fu Y C, Wang X K, Sun C Q. 2006. Identification of quantitative trait loci controlling drought tolerance at seedling stage in Chinese Dongxiang common wild rice (Oryza rufipogon Griff.). Acta Genetica Sinica, 33, 551-558.

Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage

Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage

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