Identification of QTLs for Yield-Related Traits in the Recombinant Inbred Line Population Derived from the Cross Between a Synthetic Hexaploid Wheat- Derived Variety Chuanmai 42 and a Chinese Elite Variety Chuannong 16

doi:10.1016/S1671-2927(11)60165-X

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (11): 1665-1680.DOI: 10.1016/S1671-2927(11)60165-X

Identification of QTLs for Yield-Related Traits in the Recombinant Inbred Line Population Derived from the Cross Between a Synthetic Hexaploid Wheat- Derived Variety Chuanmai 42 and a Chinese Elite Variety Chuannong 16

TANG Yong-lu, LI Jun, WU Yuan-qi, WEI Hui-ting, LI Chao-su, YANG Wu-yun , CHEN Fang

1.Crop Research Institute, Sichuan Academy of Agricultural Sciences
2.College of Life Sciences, Sichuan University
3.Agronomy College, Sichuan Agricultural University

收稿日期:2010-11-01 出版日期:2011-11-01 发布日期:2011-11-07
通讯作者: Correspondence YANG Wu-yun, Professor, Tel: +86-28-84504657, Fax: +86-28- 84790147, E-mail: yangwuyun@yahoo.com.cn
作者简介:TANG Yong-lu, Professor, Tel: +86-28-84504601, E-mai: ttyycc88@163.com
基金资助:
This work was supported by the Sichuan Provincial Youth Foundation, China (09ZQ026-086), the earmarked fund for Modern Agro-Industry Technology Research System, China (nycytx-03), the National 863 Program of China (2006AA10Z1C6), and the National Natural Science Foundation of China (30771338 and 30871532).

Identification of QTLs for Yield-Related Traits in the Recombinant Inbred Line Population Derived from the Cross Between a Synthetic Hexaploid Wheat- Derived Variety Chuanmai 42 and a Chinese Elite Variety Chuannong 16

TANG Yong-lu, LI Jun, WU Yuan-qi, WEI Hui-ting, LI Chao-su, YANG Wu-yun , CHEN Fang

1.Crop Research Institute, Sichuan Academy of Agricultural Sciences
2.College of Life Sciences, Sichuan University
3.Agronomy College, Sichuan Agricultural University

Received:2010-11-01 Online:2011-11-01 Published:2011-11-07
Contact: Correspondence YANG Wu-yun, Professor, Tel: +86-28-84504657, Fax: +86-28- 84790147, E-mail: yangwuyun@yahoo.com.cn
About author:TANG Yong-lu, Professor, Tel: +86-28-84504601, E-mai: ttyycc88@163.com
Supported by:
This work was supported by the Sichuan Provincial Youth Foundation, China (09ZQ026-086), the earmarked fund for Modern Agro-Industry Technology Research System, China (nycytx-03), the National 863 Program of China (2006AA10Z1C6), and the National Natural Science Foundation of China (30771338 and 30871532).

摘要/Abstract

摘要： Synthetic hexaploid wheat (SHW) represents a valuable source of new resistances to a range of biotic and abiotic stresses. A recombinant inbred line (RIL) population with 127 recombinant inbred lines derived from a SHW-derived variety Chuanmai 42 crossing with a Chinese spring wheat variety Chuannong 16 was used to map QTLs for agronomic traits including grain yield, grains per square meter, thousand-kernel weight, spikes per square meter, grain number per spike, grains weight per spike, and biomass yield. The population was genotyped using 184 simple-sequence repeat (SSR) markers and 34 sequence-related amplified polymorphism (SRAP) markers. Of 76 QTLs (LOD>2.5) identified, 42 were found to have a positive effect from Chuanmai 42. The QTL QGy.saas-4D.2 associated with grain yield on chromosome 4D was detected in four of the six environments and the combined analysis, and the mean yield, across six environments, of individuals carrying the Chuanmai 42 allele at this locus was 8.9% higher than that of those lines carrying the Chuannong 16 allele. Seven clusters of the yield-coincident QTLs were detected on 1A, 4A, 3B, 5B, 4D, and 7D.

Abstract: Synthetic hexaploid wheat (SHW) represents a valuable source of new resistances to a range of biotic and abiotic stresses. A recombinant inbred line (RIL) population with 127 recombinant inbred lines derived from a SHW-derived variety Chuanmai 42 crossing with a Chinese spring wheat variety Chuannong 16 was used to map QTLs for agronomic traits including grain yield, grains per square meter, thousand-kernel weight, spikes per square meter, grain number per spike, grains weight per spike, and biomass yield. The population was genotyped using 184 simple-sequence repeat (SSR) markers and 34 sequence-related amplified polymorphism (SRAP) markers. Of 76 QTLs (LOD>2.5) identified, 42 were found to have a positive effect from Chuanmai 42. The QTL QGy.saas-4D.2 associated with grain yield on chromosome 4D was detected in four of the six environments and the combined analysis, and the mean yield, across six environments, of individuals carrying the Chuanmai 42 allele at this locus was 8.9% higher than that of those lines carrying the Chuannong 16 allele. Seven clusters of the yield-coincident QTLs were detected on 1A, 4A, 3B, 5B, 4D, and 7D.

Key words: yield-related traits, quantitative trait loci, Chuanmai 42, synthetic hexaploid wheat

TANG Yong-lu, LI Jun, WU Yuan-qi, WEI Hui-ting, LI Chao-su, YANG Wu-yun , CHEN Fang. Identification of QTLs for Yield-Related Traits in the Recombinant Inbred Line Population Derived from the Cross Between a Synthetic Hexaploid Wheat- Derived Variety Chuanmai 42 and a Chinese Elite Variety Chuannong 16 [J]. Journal of Integrative Agriculture, 2011, 10(11): 1665-1680.

参考文献

[1]Araki E, Miura H, Sawada S. 1999. Identification of genetic loci affecting amylose content and agronomic traits on chromosome 4A of wheat. Theoretical and Applied Genetics, 98, 977-984.

[2]Assefa S, Fehrmann H. 2004. Evaluation of Aegilops tauschii Coss. for resistance to wheat stem rust and inheritance of resistance genes in hexaploid wheat. Genetic Resources and Crop Evolution, 51, 663-669.

[3]Bassam B J, Caetano A G, Gresshoff P M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196, 80-83.

[4]del Blanco I A, Rajaram S, Kronstad W E, Reynolds M P. 2000. Physiological performance of synthetic hexaploid wheatderived populations. Crop Science, 40, 1257-1263.

[5]del Blanco I A, Rajaram S, Kronstad W E. 2001. Agronomic potential of synthetic hexaploid wheat-derived populations. Crop Science, 41, 670-676.

[6]Börner A, Schumann E, Furste A, Coster H, Leithold B, Röder M S, Weber W E. 2002. Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 105, 921-936.

[7]Campbell B T, Baenziger P S, Gill K S, Eskridge K M, Budak H, Erayman M, Dweikat I, Yen Y. 2003. Identification of QTLs and environmental interactions associated with agronomic traits on chromosome 3A of wheat. Crop Science, 43, 1493- 1505.

[8]Cakmak I, Tolay I, Ozdemir A, Ozkan H, Ozturk L, Kling C I. 1999. Differences in zinc efficiency among and within diploid, tetraploid and hexaploid wheats. Annals of Botany, 84, 163- 171.

[9]Dreccer M F, Borgognone M G, Ogbonnaya F C, Trethowan R M, Winter B. 2007. CIMMYT-selected derived synthetic bread wheat for rain fed environments: yield evaluation in Mexico and Australia. Field Crops Research, 100, 218-228.

[10]Gautier M F, Cosson P, Guirao A, Alary R, Joudrier P. 2000. Puroindoline genes are highly conserved in diploid ancestor wheats and related species but absent in tetraploid Triticum species. Plant Science, 153, 81-91.

[11]van Ginkel M, Francis O. 2007. Novel genetic diversity from synthetic wheats in breeding cultivars for changing production conditions. Field Crops Research, 104, 86-94.

[12]Gororo N N, Eagles H A, Eastwood R F, Nicolas M E, Flood R G. 2002. Use of Triticum tauschii to improve yield of wheat in low-yielding environments. Euphytica, 123, 241-254.

[13]Gupta P K, Balyan H S, Edwards K J, Isaac P, Korzun V, Röder M S, Gautier M F, Joudrier P, Schlatter A R, Dubcovsky J, et al. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theoretical and Applied Genetics, 105, 413-422.

[14]Guyomarc’h H, Sourdille P, Charmet G, Edwards K J, Bernard M. 2002. Characterization of polymorphic microsatellite markers from Aegilops tauschii and transferability to the Dgenome of bread wheat. Theoretical and Applied Genetics, 104, 1164-1172.

[15]Hegde S G, Waines J G. 2004. Hybridization and introgression between bread wheat and wild and weedy relatives in North America. Crop Science, 44, 1145-1155.

[16]Huang X Q, Hsam S L K, Zeller F J, Wenzel G, Mohler V. 2000. Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theoretical and Applied Genetics, 101, 407-414.

[17]Huang X Q, Coster H, Ganal M W, Röder M S. 2003. Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 106, 1379- 1389.

[18]Huang X Q, Kempf H, Ganal M W, Röder M S. 2004. Advanced backcross QTL analysis in progenies derived from a cross between a German elite winter wheat variety and a synthetic wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109, 933-943.

[19]Huang X Q, Cloutier S, Lycar L, Radovanovic N, Humphreys D G, Noll J S, Somers D J, Brown P D. 2006. Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.). Theoretical and Applied Genetics, 113, 753-766.

[20]Kato K, Miura H, Sawada S. 2000. Mapping QTLs controlling grain yield and its components on chromosome 5A of wheat. Theoretical and Applied Genetics, 101, 1114-1121.

[21]Kosambi D D. 1944. The estimation of map distances from recombination values. Annals of Eugenics, 12, 172-175.

[22]Kirigwi F M, van Ginkel M, Brown-Guedira G, Gill B S, Paulsen G M, Fritz A K. 2007. Markers associated with a QTL for grain yield in wheat under drought. Molecular Breeding, 20, 401-413.

[23]Kuchel H, Williams K J, Langridge P, Eagles H A, Jefferies S P . 2007a. Genetic dissection of grain yield in bread wheat. I. QTL analysis. Theoretical and Applied Genetics, 115, 1029- 1041.

[24]Kuchel H, Williams K J, Langridge P, Eagles H A, JeVeries S P . 2007b. Genetic dissection of grain yield in bread wheat. II. QTL-by-environment interaction. Theoretical and Applied Genetics, 115, 1015-1027.

[25]Lan C X, Ni, X W, Yan J, Zhang Y, Xia X C, Chen X M, He Z H. 2010. Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21. Molecular Breeding, 25, 615-622.

[26]Lan X J, Yan J. 1992. An amphidiploid derived from a Chinese landrace of tetraploid wheat, ailanmai crossed with Aegilops tauschii native to China and with reference to its utilization in wheat breeding. Journal of Sichuan Agricultural University, 4, 581-585. (in Chinese)

[27]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1, 174-181.

[28]Li S S, Jia J S, Wei X Y, Zhang X C, Li L Z, Chen H M, Fan Y D, Sun H Y, Zhao X H, Lei T D, et al. 2007. A intervarietal genetic map and QTL analysis for yield traits in wheat. Molecular Breeding, 20, 167-178.

[29]Li G, Quiros C F. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 103, 455-461.

[30]Li H H, Ye G Y, Wang J K. 2007. A modified algorithm for the improvement of composite interval mapping. Genetics, 175, 361-374.

[31]Li J, Wei H T, Yang S J, Li C S, Tang Y L, Hu X R, Yang W Y. 2009. Genetic effects of 1BS chromosome arm on the main agronomic traits in Chuanmai 42. Acta Agronomica Sinica, 35, 1-7. (in Chinese)

[32]Li J, Wei H T, Hu X R, Li C S, Tang Y L, Liu D C, Yang W Y. 2010. Identification of a high-yield introgression locus from synthetic hexaploid wheat in Chuanmai 42. Acta Agronomica Sinica, 37, 1-8. (in Chinese)

[33]Liao J, Wei H T, Li J, Yang Y M, Zeng Y C, Peng Z S, Yang W Y. 2007. Detection of the introgression loci of synthetic hexaploid wheat in wheat cultivar Chuanmai 42 by SSR markers. Acta Agronomica Sinica, 5, 703-707. (in Chinese)

[34]Marza F, Bai G H, Carver B Y, Zhou W C. 2006. Quantitative trait loci for yield and related traits in the wheat population Ning7840×Clark. Theoretical and Applied Genetics, 112, 688- 698.

[35]McCartney C A, Somers D J, Humphreys D G, Lukow O, Ames N, Noll J, Cloutier S, McCallum B D. 2005. Mapping quantitative trait loci controlling agronomic traits in the spring wheat cross RL4452×‘AC Domain’. Genome, 48, 870-883.

[36]Narasimhamoorthy B, Gill B S, Fritz A K, Nelson J C, Brown- Guedira G L. 2006. Advanced backcross QTL analysis of a hard winter wheat×synthetic wheat population. Theoretical and Applied Genetics, 112, 787-796.

[37]Ogbonnaya F C, Ye G Y, Trethowan R, Dreccer F, Lush D, Shepperd J, van Ginkel M. 2007. Yield of synthetic backcrossderived lines in rain fed environments of Australia. Euphytica, 3, 321-336.

[38]Pestsova E, Ganal M W, Röder M S. 2000. Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome, 43, 689-697.

[39]Quarrie S A, Steed A, Calestani C, Semikhodskii A, Lebreton C, Chinoy C, Steele N, Pljevljakusi D, Waterman E, Weyen J, et al. 2005. A high-density genetic map of hexaploid wheat (Triticum aestivum L.) from the cross Chinese Spring×SQ1 and its use to compare QTLs for grain yield across a range of environments. Theoretical and Applied Genetics, 110, 865- 880.

[40]Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. 1998. A microsatellite map of wheat. Genetics, 149, 2007-2023.

[41]SAS Institute. 1988. SAS User Guide: Statistics. SAS Institute, Cary, NC. Slafer G A. 1996. Differences in phasic development rate amongst wheat cultivars independent of responses to photoperiod and vernalization: a viewpoint of the intrinsic earliness hypothesis. The Journal of Agricultural Science, 126, 403- 419.

[42]Snape J W, Foulkes M J, Simmonds J, Leverington M, Fish L J, Wang Y K, Ciavarrella M. 2007. Dissecting gene 3 environmental effects on wheat yields via QTL and physiological analysis. Euphytica, 154, 401-408.

[43]Somers D J, Isaac P, Edwards K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109, 1105- 1114.

[44]Song Q J, Shi J R, Singh S, Fickus E W, Mosta J M , Lewis J, Gill B S, Ward R, Cregan P B. 2005. Development and mapping of microsatellite (SSR) markers in wheat. Theoretical and Applied Genetics, 110, 550-560.

[45]Swamy B P M, Sarla N. 2008. Yield-enhancing quantitative trait loci (QTLs) from wild species. Biotechnollgy Advances, 26, 106-120.

[46]Tang Y L, Yang W Y, Huang G, Hu X R. 2006. Study on the yield potential of two wheat cultivars with different spike type in Sichuan basin. Southwest China Journal of Agricultural Sciences, 19, 339-341. (in Chinese)

[47]Tang Y L, Zhu H Z, Li C S, Huang G, Yu X F, Chen F, Yang W Y. 2007. Study on ecological adaptability and yield potential of new wheat cultivar “Chuanmai 42” derived from synthetic hexaploid wheat. Southwest China Journal of Agricultural Sciences, 20, 275-280. (in Chinese)

[48]Wang S C, Basten C J, Zeng Z B. 2011. Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC. http://statgen.ncsu.edu/qtlcart/ WQTLCart.htm

[49]Wang R X, Hai L, Zhang X Y, You G X, Yan C S, Xiao X H. 2009. QTL mapping for grain filling rate and yield-related traits in RILs of the Chinese winter wheat population Heshanmai × Yu8679. Theoretical and Applied Genetics, 118, 313-325.

[50]Yang P, Liu X J, Liu X C, Li J, Wang X W, He S P, Li G, Yang W Y, Peng Z Y. 2008. Genetic diversity analysis of the developed qingke (hulless barley) varieties from the plateau regions of Sichuan Province in China revealed by SRAP markers. Hereditas, 30, 115-122. (in Chinese)

[51]Yang W Y, Lu B R, Yi Y, Hu X R. 2001. Genetic evaluation of synthetic hexaploid wheat for resistance to the physiological strain CYR30 and CYR31 of wheat stripe rust in China. Journal of Genetics and Molecular Genetics, 12, 190-198.

[52]Yang W Y, Li J, Hu X R. 2007. Crossability of 102 CIMMYT synthetic hexaploid wheats with rye. Southwest China Journal of Agricultural Sciences, 20, 218-224. (in Chinese)

[53]Yang W Y, Liu D C, Li J, Zhang Z Q, Wei H T, Hu X R, Zheng L Y, He Z H, Zou Y C. 2009. Synthetic hexaploid wheat and its utilization for wheat genetic improvement in China. Journal of Genetics and Genomics, 36, 539-546.

[54]Zeng Z B. 1994. Precision mapping of quantitative trait loci. Genetics, 136, 1457-1468.

[55]Zhang Y, Yang W Y, Hu X R, Yu Y, Zou Y C, Li Q M. 2004. Analysis of agronomic characters of new wheat variety Chuanmai 42 derived from synthetics (Triticum durum× Aegilops tauschii). Southwest China Journal of Agricultural Sciences, 2, 141-145. (in Chinese)

Identification of QTLs for Yield-Related Traits in the Recombinant Inbred Line Population Derived from the Cross Between a Synthetic Hexaploid Wheat- Derived Variety Chuanmai 42 and a Chinese Elite Variety Chuannong 16

Identification of QTLs for Yield-Related Traits in the Recombinant Inbred Line Population Derived from the Cross Between a Synthetic Hexaploid Wheat- Derived Variety Chuanmai 42 and a Chinese Elite Variety Chuannong 16

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