Effects of Consecutively Monocultured Rehmannia glutinosa L. on Diversity of Fungal Community in Rhizospheric Soil

doi:10.1016/S1671-2927(11)60130-2

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (9): 1374-1384.DOI: 10.1016/S1671-2927(11)60130-2

Effects of Consecutively Monocultured Rehmannia glutinosa L. on Diversity of Fungal Community in Rhizospheric Soil

ZHANGZhong-yi, LINWen-xiong, YANGYan-hui, CHENHui, CHENXin-jian

1.Engineering Research Center of Chinese Medicinal Materials
2.Institute of Agroecology, Fujian Agriculture and Forestry University

收稿日期:2010-10-13 出版日期:2011-09-01 发布日期:2011-09-09
通讯作者: Correspondence LIN Wen-xiong, Professor, E-mail:wenxiong181@163.com
作者简介:ZHANG Zhong-yi, Ph D, Professor, Tel: +86-371-63555985, Fax: +86-371-63555985, E-mail: hauzzy@163.com
基金资助:
This work was supported by the National Natural ScienceFoundation of China (30772729, 30671201, and81072983), and the Key Technologies R&D Programof China during the 11th Five-Year Plan period(2006BAI09B03 and 2006BAI06A12-06).

Effects of Consecutively Monocultured Rehmannia glutinosa L. on Diversity of Fungal Community in Rhizospheric Soil

ZHANGZhong-yi, LINWen-xiong, YANGYan-hui, CHENHui, CHENXin-jian

1.Engineering Research Center of Chinese Medicinal Materials
2.Institute of Agroecology, Fujian Agriculture and Forestry University

Received:2010-10-13 Online:2011-09-01 Published:2011-09-09
Contact: Correspondence LIN Wen-xiong, Professor, E-mail:wenxiong181@163.com
About author:ZHANG Zhong-yi, Ph D, Professor, Tel: +86-371-63555985, Fax: +86-371-63555985, E-mail: hauzzy@163.com
Supported by:
This work was supported by the National Natural ScienceFoundation of China (30772729, 30671201, and81072983), and the Key Technologies R&D Programof China during the 11th Five-Year Plan period(2006BAI09B03 and 2006BAI06A12-06).

摘要/Abstract

摘要： Continuous monoculture problems, or replanting diseases, are one of the key factors affecting productivity and quality ofChinese medicinal plants. The underlying mechanism is still being explored. Most of the studies on continuous monocultureof Rehmannia glutinosa L. are focused on plant nutritional physiology, root exudate, and its autotoxicity. However, thechanges in the diversity of microflora in the rhizosphere mediated by the continuous monoculture pattern have beenremained unknown. In this study, terminal restriction fragment length polymorphism (T-RFLP) technique was used forfingerprinting fungal diversity in the rhizosphere soil sampled from the fields of R. glutinosa monocultured for 1 and 2 yr. Theresults showed that the structure of fungal community in consecutively moncultured rhizosphere soil was different fromthat in control soil (no cropping soil), and varied with the consecutive monoculture years (1 and 2 yr). The comprehensiveevaluation index (D) of fungal community estimated by principal component analysis of fragment number, peak area,Shannon-Weiner index, and Margalef index was higher in 1 yr monoculture soil than that in 2 yr monoculture soil,suggesting that consecutive monoculture of R. glutinosa could be a causative agent to decrease the diversity of fungalcommunity in the rhizosphere soil.

关键词: biodiversity, consecutive monoculture problem, fungal community, Rehmannia glutinosa, rhizosphere soil, T-RFLP

Abstract: Continuous monoculture problems, or replanting diseases, are one of the key factors affecting productivity and quality ofChinese medicinal plants. The underlying mechanism is still being explored. Most of the studies on continuous monocultureof Rehmannia glutinosa L. are focused on plant nutritional physiology, root exudate, and its autotoxicity. However, thechanges in the diversity of microflora in the rhizosphere mediated by the continuous monoculture pattern have beenremained unknown. In this study, terminal restriction fragment length polymorphism (T-RFLP) technique was used forfingerprinting fungal diversity in the rhizosphere soil sampled from the fields of R. glutinosa monocultured for 1 and 2 yr. Theresults showed that the structure of fungal community in consecutively moncultured rhizosphere soil was different fromthat in control soil (no cropping soil), and varied with the consecutive monoculture years (1 and 2 yr). The comprehensiveevaluation index (D) of fungal community estimated by principal component analysis of fragment number, peak area,Shannon-Weiner index, and Margalef index was higher in 1 yr monoculture soil than that in 2 yr monoculture soil,suggesting that consecutive monoculture of R. glutinosa could be a causative agent to decrease the diversity of fungalcommunity in the rhizosphere soil.

Key words: biodiversity, consecutive monoculture problem, fungal community, Rehmannia glutinosa, rhizosphere soil, T-RFLP

ZHANG Zhong-yi, LIN Wen-xiong, YANG Yan-hui, CHEN Hui, CHEN Xin-jian. Effects of Consecutively Monocultured Rehmannia glutinosa L. on Diversity of Fungal Community in Rhizospheric Soil[J]. Journal of Integrative Agriculture, 2011, 10(9): 1374-1384.

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Effects of Consecutively Monocultured Rehmannia glutinosa L. on Diversity of Fungal Community in Rhizospheric Soil

Effects of Consecutively Monocultured Rehmannia glutinosa L. on Diversity of Fungal Community in Rhizospheric Soil

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