Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

doi:10.1016/S1671-2927(11)60131-4

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (9): 1385-1390.DOI: 10.1016/S1671-2927(11)60131-4

Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

WANG Ying, JIAO Tian-lei, LIU Xiao-hong, LIN Fu-cheng , WU Wei-ren

1. Department of Agronomy, College of Agriculture & Biotechnology
2. Institute of Biotechnology, College of Agriculture & Biotechnology
3. School of Life Sciences, Fujian Agriculture & Forestry University

收稿日期:2010-12-08 出版日期:2011-09-01 发布日期:2011-09-09
通讯作者: Correspondence WU Wei-ren, Professor, Tel/Fax: +86-571-86971910, E-mail: wuwr@zju.edu.cn; LIN Fu-cheng, Professor,Tel/Fax: +86-571-86971185, E-mail: fuchenglin@zju.edu.cn
作者简介:WANG Ying, Ph D, E-mail: wyhrx@126.com
基金资助:
the National Natural Science Foundation of China (30671123 and 31000077).

Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

WANG Ying, JIAO Tian-lei, LIU Xiao-hong, LIN Fu-cheng , WU Wei-ren

1. Department of Agronomy, College of Agriculture & Biotechnology
2. Institute of Biotechnology, College of Agriculture & Biotechnology
3. School of Life Sciences, Fujian Agriculture & Forestry University

Received:2010-12-08 Online:2011-09-01 Published:2011-09-09
Contact: Correspondence WU Wei-ren, Professor, Tel/Fax: +86-571-86971910, E-mail: wuwr@zju.edu.cn; LIN Fu-cheng, Professor,Tel/Fax: +86-571-86971185, E-mail: fuchenglin@zju.edu.cn
About author:WANG Ying, Ph D, E-mail: wyhrx@126.com
Supported by:
the National Natural Science Foundation of China (30671123 and 31000077).

摘要/Abstract

摘要： Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With thetechnology of suppression subtractive hybridization (SSH), it was previously found that MGG_06001 (or named MoNEM1),a gene of M. oryzae homologous to the NEM1 (nuclear envelope morphology protein 1) gene of baker’s yeast(Saccharomyces cerevisiae), is differentially expressed between the mature appressium and the conidium and mycelium.This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target genereplacement. The ΔMonem1 mutants exhibited reduced mycelial growth and conidiation. However, disruption of MoNEM1gene does not affect the pathogenicity of M. oryzae on barley and rice.

关键词:

Magnaporthe oryzae, MoNEM1, conidiation, growth, pathogenicity

Abstract: Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With thetechnology of suppression subtractive hybridization (SSH), it was previously found that MGG_06001 (or named MoNEM1),a gene of M. oryzae homologous to the NEM1 (nuclear envelope morphology protein 1) gene of baker’s yeast(Saccharomyces cerevisiae), is differentially expressed between the mature appressium and the conidium and mycelium.This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target genereplacement. The ΔMonem1 mutants exhibited reduced mycelial growth and conidiation. However, disruption of MoNEM1gene does not affect the pathogenicity of M. oryzae on barley and rice.

Key words:

Magnaporthe oryzae, MoNEM1, conidiation, growth, pathogenicity

WANG Ying, JIAO Tian-lei, LIU Xiao-hong, LIN Fu-cheng , WU Wei-ren. Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae[J]. Journal of Integrative Agriculture, 2011, 10(9): 1385-1390.

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Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

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