Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (7): 1010-1015.DOI: 10.1016/S1671-2927(11)60088-6

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Expression Analysis of Restorer Alleles-Induced Genes in Pepper

  

  1. Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University
  • 收稿日期:2010-07-28 出版日期:2011-07-21 发布日期:2011-08-14
  • 通讯作者: Correspondence SHEN Huo-lin, Tel: +86-10-62732831, E-mail: SHL1606@cau.edu.cn
  • 作者简介:GUO Shuang, Ph D, E-mail: guoshuang6286757@163.com

Expression Analysis of Restorer Alleles-Induced Genes in Pepper

  1. Department of Vegetable Science, College of Agronomy and Biotechnology, China Agricultural University
  • Received:2010-07-28 Online:2011-07-21 Published:2011-08-14
  • Contact: Correspondence SHEN Huo-lin, Tel: +86-10-62732831, E-mail: SHL1606@cau.edu.cn
  • About author:GUO Shuang, Ph D, E-mail: guoshuang6286757@163.com

摘要: Fertility restoration of cytoplasmic male-sterility in pepper (Capsicum annuum L.) is useful for commercial production ofhybrid seeds. However, the mechanism of fertility restoration has not been determined. We previously constructed acDNA library and identified some genes related to fertility restoration in pepper using suppression subtractive hybridizationtechnology. In this study, the expression patterns of 20 genes were investigated using semi-quantitative RT-PCR. Threegenes expressed only in restorer lines, but not in sterility lines. Four genes expressed only in anther, but not in otherorgans. Among these 7 genes, the clone TG31 was observed to specifically express in anther of restorer lines. The workdescribed here provides a comprehensive overview on the expression pattern of the genes that are induced by restoreralleles in pepper. It will also contribute to the current understanding of molecular networks for the regulation of fertilityrestoration.

关键词:

Abstract: Fertility restoration of cytoplasmic male-sterility in pepper (Capsicum annuum L.) is useful for commercial production ofhybrid seeds. However, the mechanism of fertility restoration has not been determined. We previously constructed acDNA library and identified some genes related to fertility restoration in pepper using suppression subtractive hybridizationtechnology. In this study, the expression patterns of 20 genes were investigated using semi-quantitative RT-PCR. Threegenes expressed only in restorer lines, but not in sterility lines. Four genes expressed only in anther, but not in otherorgans. Among these 7 genes, the clone TG31 was observed to specifically express in anther of restorer lines. The workdescribed here provides a comprehensive overview on the expression pattern of the genes that are induced by restoreralleles in pepper. It will also contribute to the current understanding of molecular networks for the regulation of fertilityrestoration.

Key words: pepper, restorer alleles, semi-quantitative RT-PCR, expression