The Effect ofAdministration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats

doi:10.1016/S1671-2927(00)8703

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (10): 1700-1706.DOI: 10.1016/S1671-2927(00)8703

The Effect ofAdministration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats

GUO Xu-dong, DIAO Qi-yu, WANG Yue-ying, TU Yan, DENG Kai-dong, WANG Xin-jian, FU Tong, YAN Gui-long

1.Key Laboratory of Feed Biotechnology, Ministry of Agriculture/Feed Research Institute, Chinese Academy of Agricultural Sciences,Beijing 100081, P.R.China
2.Key Laboratory of Animal Growth and Development, Ministry of Agriculture/Henan Agricultual University, Zhengzhou 450002, P.R.China
3.College of Animal Sciences, Jinling Institute of Technology, Nanjing 210038, P.R.China
4.College of Animal Sciences, Hebei North University, Zhangjiakou 075000, P.R.China

收稿日期:2011-05-31 出版日期:2012-10-01 发布日期:2012-11-12
通讯作者: Correspondence DIAO Qi-yu, E-mail: diaoqiyu@mail.caas.net.cn
作者简介:GUO Xu-dong, E-mail: gxd.168@163.com
基金资助:
This study was funded by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2006BAD12B04-04).

The Effect ofAdministration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats

GUO Xu-dong, DIAO Qi-yu, WANG Yue-ying, TU Yan, DENG Kai-dong, WANG Xin-jian, FU Tong, YAN Gui-long

1.Key Laboratory of Feed Biotechnology, Ministry of Agriculture/Feed Research Institute, Chinese Academy of Agricultural Sciences,Beijing 100081, P.R.China
2.Key Laboratory of Animal Growth and Development, Ministry of Agriculture/Henan Agricultual University, Zhengzhou 450002, P.R.China
3.College of Animal Sciences, Jinling Institute of Technology, Nanjing 210038, P.R.China
4.College of Animal Sciences, Hebei North University, Zhangjiakou 075000, P.R.China

Received:2011-05-31 Online:2012-10-01 Published:2012-11-12
Contact: Correspondence DIAO Qi-yu, E-mail: diaoqiyu@mail.caas.net.cn
About author:GUO Xu-dong, E-mail: gxd.168@163.com
Supported by:
This study was funded by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2006BAD12B04-04).

摘要/Abstract

摘要： The development of mammary glands, endocrine hormone concentrations and the gene expression of related receptors were measured in ovariectomized virgin rats after adminstration of an estrogen-like plant extract, rutin. Thirty-two ovariectomized virgin Wistar rats were randomly assigned to 4 treatments with 8 animals each: gastric infusion of 2 mL normal saline per unovariectomized rat per day (Sham), gastric infusion of 2 mL normal saline per ovariectomized rat per day (Ova), gastric infusion of 60 mg rutin kg-1 body weight (BW) per ovariectomized rat per day (Ova+Rut), or intramuscular injection of 60 μg estradiol kg-1 BW per ovariectomized rat weekly (Ova+Est). Samples of blood and mammary glands were harvested to determine the levels of estrogen (E2), prolactin (PRL) and growth hormone (GH), and the gene expression of estrogen receptors (ER), prolactin receptors (PRLR) and growth hormone receptors (GHR) with radioimmunoassy (RIA) and RT-PCR technology, respectively. The E2 concentration in plasma and gland tissues from the rats of Ovx+Rut or Ovx+Est was higher than that of Ovx (P<0.05), but the plasma E2 concentration from the rats of Ovx+Rut was lower than that of Sham (P<0.05). The order of the PRL concentration in plasma and gland tissues was Ovx

关键词: rutin , rats , mammary gland development , hormones , receptors

Abstract: The development of mammary glands, endocrine hormone concentrations and the gene expression of related receptors were measured in ovariectomized virgin rats after adminstration of an estrogen-like plant extract, rutin. Thirty-two ovariectomized virgin Wistar rats were randomly assigned to 4 treatments with 8 animals each: gastric infusion of 2 mL normal saline per unovariectomized rat per day (Sham), gastric infusion of 2 mL normal saline per ovariectomized rat per day (Ova), gastric infusion of 60 mg rutin kg-1 body weight (BW) per ovariectomized rat per day (Ova+Rut), or intramuscular injection of 60 μg estradiol kg-1 BW per ovariectomized rat weekly (Ova+Est). Samples of blood and mammary glands were harvested to determine the levels of estrogen (E2), prolactin (PRL) and growth hormone (GH), and the gene expression of estrogen receptors (ER), prolactin receptors (PRLR) and growth hormone receptors (GHR) with radioimmunoassy (RIA) and RT-PCR technology, respectively. The E2 concentration in plasma and gland tissues from the rats of Ovx+Rut or Ovx+Est was higher than that of Ovx (P<0.05), but the plasma E2 concentration from the rats of Ovx+Rut was lower than that of Sham (P<0.05). The order of the PRL concentration in plasma and gland tissues was Ovx

Key words: rutin , rats , mammary gland development , hormones , receptors

GUO Xu-dong, DIAO Qi-yu, WANG Yue-ying, TU Yan, DENG Kai-dong, WANG Xin-jian, FU Tong, YAN Gui-long. The Effect ofAdministration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats[J]. Journal of Integrative Agriculture, 2012, 12(10): 1700-1706.

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The Effect ofAdministration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats

The Effect ofAdministration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats

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