Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China

doi:10.1016/S1671-2927(00)8517

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (1): 116-121.DOI: 10.1016/S1671-2927(00)8517

Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China

GUO Li-li, ZHANG Jian-min, XU Cheng-gang, REN Tao, ZHANG Bin, CHEN Ji-dang , LIAO Ming

1.Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China

收稿日期:2010-12-03 出版日期:2012-01-01 发布日期:2012-01-13
通讯作者: Correspondence LIAO Ming, Tel: +86-20-85280240, Fax: +86-20-85285282, E-mail: mliao@scau.edu.cn
作者简介:GUO Li-li, E-mail: gll2004203746@126.com
基金资助:
This work was supported by the Program for New Century Excellent Talents in University, China (NCET-06-0752), and the Guangdong Technology Planning Committee, China (2006B0152 and 2009A0201006).

Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China

GUO Li-li, ZHANG Jian-min, XU Cheng-gang, REN Tao, ZHANG Bin, CHEN Ji-dang , LIAO Ming

1.Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China

Received:2010-12-03 Online:2012-01-01 Published:2012-01-13
Contact: Correspondence LIAO Ming, Tel: +86-20-85280240, Fax: +86-20-85285282, E-mail: mliao@scau.edu.cn
About author:GUO Li-li, E-mail: gll2004203746@126.com
Supported by:
This work was supported by the Program for New Century Excellent Talents in University, China (NCET-06-0752), and the Guangdong Technology Planning Committee, China (2006B0152 and 2009A0201006).

摘要/Abstract

摘要： To characterize the β-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the β-lactam resistance, the possession of β-lactamase, and the presence of β-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis (PFGE). Overall, the commonly detected resistance phenotypes were resistant to ampicillin (26.09%), penicillin (22.61%), amoxicillin (21.74%), cefazolin (14.78%), cefaclor (12.17%), and cefotaxime (6.96%). These strains showed high minimal inhibitory concentration (MICs) to oxacillin. 20.87% strains produced β-lactamase, and 4.35% strains showed extended-spectrum b-lactamase (ESBL) phenotype. Moreover, 19 strains harboured bla genes including TEM-1 (n=5), TEM-116 (n=10), and ROB-1 (n=5). Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime (MIC=8 mg L-1). The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type β-lactamases are prevalent in H. parasuis isolates in China.

Abstract: To characterize the β-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the β-lactam resistance, the possession of β-lactamase, and the presence of β-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis (PFGE). Overall, the commonly detected resistance phenotypes were resistant to ampicillin (26.09%), penicillin (22.61%), amoxicillin (21.74%), cefazolin (14.78%), cefaclor (12.17%), and cefotaxime (6.96%). These strains showed high minimal inhibitory concentration (MICs) to oxacillin. 20.87% strains produced β-lactamase, and 4.35% strains showed extended-spectrum b-lactamase (ESBL) phenotype. Moreover, 19 strains harboured bla genes including TEM-1 (n=5), TEM-116 (n=10), and ROB-1 (n=5). Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime (MIC=8 mg L-1). The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type β-lactamases are prevalent in H. parasuis isolates in China.

Key words: Haemophilus parasuis, β-lactamase genes, resistance, pulsed-field gel electrophoresis

GUO Li-li, ZHANG Jian-min, XU Cheng-gang, REN Tao, ZHANG Bin, CHEN Ji-dang , LIAO Ming. Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China[J]. Journal of Integrative Agriculture, 2012, 12(1): 116-121.

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Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China

Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China

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