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1. Response and adaptation to the accumulation and distribution of photosynthetic product in peanut under salt stress
ZHANG Guan-chu, DAI Liang-xiang, DING Hong, CI Dun-wei, NING Tang-yuan, YANG Ji-shun, ZHAO Xin-hua, YU Hai-qiu, ZHANG Zhi-meng
Journal of Integrative Agriculture    2020, 19 (3): 690-699.   DOI: 10.1016/S2095-3119(19)62608-0
摘要168)      PDF    收藏

本研究以花育25为材料,设置非盐胁迫(CK),0.15%(S1)和0.3%盐胁迫(S2)三个处理,探究了盐胁迫对花生光合特性、植株不同器官干物质积累与分配及荚果发育动态的影响。结果表明,盐胁迫降低了花生的净光合速率(Pn),SPAD值,单株叶面积和产量;播种后第50天,CK的Pn分别较S1和S2处理高出13.71%和28.72%。同一生育期内,处理间的SPAD值差异均为CK>S1>S2。盐胁迫降低了花生的单株荚果重,百果重,百仁重和出米率,大小为CK>S1>S2。与CK相较,花生通过改变了光合产物在不同器官中的分配比例来适应盐胁迫,在营养生长和早期生殖期生长阶段,S1和S2处理增加了光合产物在茎和荚果中分配比例。整个生育期内,S1和S2处理的根系干重集中分布在0-40厘米的土壤层中。荚果发育过程中,S1和S2处理的荚果体积、干重和果仁干重的最大生长速率(Vmax)均下降;收获时,S1和S2处理的荚果和果仁的体积较CK均降低,因此盐胁迫下花生通过降低荚果和果仁体积来提高饱满度。以上发现为盐碱地种植花生提供了理论指导


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2. Effects of potassium deficiency on photosynthesis, chloroplast ultrastructure, ROS, and antioxidant activities in maize (Zea mays L.)
DU Qi, ZHAO Xin-hua, XIA Le, JIANG Chun-ji, WANG Xiao-guang, HAN Yi, WANG Jing, YU Hai-qiu
Journal of Integrative Agriculture    2019, 18 (2): 395-406.   DOI: 10.1016/S2095-3119(18)61953-7
摘要342)      PDF(pc) (5041KB)(821)    收藏
Potassium (K) deficiency significantly decreases photosynthesis due to leaf chlorosis induced by accumulation of reactive oxygen species (ROS).  But, the physiological mechanism for adjusting antioxidative defense system to protect leaf function in maize (Zea mays L.) is unknown.  In the present study, four maize inbred lines (K-tolerant, 90-21-3 and 099; K-sensitive, D937 and 835) were used to analyze leaf photosynthesis, anatomical structure, chloroplast ultrastructure, ROS, and antioxidant activities.  The results showed that the chlorophyll content, net photosynthetic rate (Pn), stomatal conductance (Gs), photochemical quenching (qP), and electron transport rate of PSII (ETR) in 90-21-3 and 099 were higher than those in D937 and 835 under K deficiency treatment.  Parameters of leaf anatomical structure in D937 that were significantly changed under K deficiency treatment include smaller thickness of leaf, lower epidermis cells, and vascular bundle area, whereas the vascular bundle area, xylem vessel number, and area in 90-21-3 were significantly larger or higher.  D937 also had seriously damaged chloroplasts and PSII reaction centers along with increased superoxide anion (O2-·) and hydrogen peroxide (H2O2).  Activities of antioxidants, like superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), were significantly stimulated in 90-21-3 resulting in lower levels of O2-· and H2O2.  These results indicated that the K-tolerant maize promoted antioxidant enzyme activities to maintain ROS homeostasis and suffered less oxidative damage on the photosynthetic apparatus, thereby maintaining regular photosynthesis under K deficiency stress.
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3. Response of root morphology, physiology and endogenous hormones in maize (Zea mays L.) to potassium deficiency
ZHAO Xin-hua, YU Hai-qiu, WEN Jing, WANG Xiao-guang, DU Qi, WANG Jing, WANG Qiao
Journal of Integrative Agriculture    2016, 15 (4): 785-794.   DOI: 10.1016/S1671-2927(00)10445
摘要1525)      PDF    收藏
Potassium (K) deficiency is one of the major abiotic stresses which has drastically influenced maize growth and yield around the world. However, the physiological mechanism of K deficiency tolerance is not yet fully understood. To identify the differences of root morphology, physiology and endogenous hormones at different growing stages, two maize inbred lines 90-21-3 (tolerance to K deficiency) and D937 (sensitive to K deficiency) were cultivated in the long-term K fertilizer experimental pool under high potassium (+K) and low potassium (–K) treatments. The results indicated that the root length, volume and surface area of 90-21-3 were significantly higher than those of D937 under –K treatment at different growing stages. It was noteworthy that the lateral roots of 90-21-3 were dramatically higher than those of D937 at tasselling and flowering stage under –K treatment. Meanwhile, the values of superoxide dismutase (SOD) and oxidizing force of 90-21-3 were apparently higher than those of D937, whereas malondialdehyde (MDA) content of D937 was obviously increased. Compared with +K treatment, the indole-3-acetic acid (IAA) content of 90-21-3 was largely increased under –K treatment, whereas it was sharply decreased in D937. On the contrary, abscisic acid (ABA) content of 90-21-3 was slightly increased, but that of D937 was significantly increased. The zeatin riboside (ZR) content of 90-21-3 was significantly decreased, while that of D937 was relatively increased. These results indicated that the endogenous hormones were stimulated in 90-21-3 to adjust lateral root development and to maintain the physiology function thereby alleviating K deficiency.
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4. Effects of potassium deficiency on photosynthesis and photoprotection mechanisms in soybean (Glycine max (L.) Merr.)
WANG Xiao-guang, ZHAO Xin-hua, JIANG Chun-ji, LI Chun-hong, CONG Shan, WU Di, CHEN Yan-qiu, YU Hai-qiu, WANG Chun-yan
Journal of Integrative Agriculture    2015, 14 (5): 856-863.   DOI: 10.1016/S2095-3119(14)60848-0
摘要2435)      PDF    收藏
Potassium is an important nutrient element requiring high concentration for photosynthetic metabolism. The potassium deficiency in soil could inhibit soybean (Glycine max (L.) Merr.) photosynthesis and result in yield reduction. Research on the photosynthetic variations of the different tolerant soyben varieties should provide important information for high yield tolerant soybean breeding program. Two representative soybean varieties Tiefeng 40 (tolerance to K+ deficiency) and GD8521 (sensitive to K+ deficiency) were hydroponically grown to measure the photosynthesis, chlorophyll fluorescence parameters and Rubisco activity under different potassium conditions. With the K-deficiency stress time extending, the net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) of GD8521 were significantly decreased under K-deficiency condition, whereas the intercellular CO2 concentration (Ci) was significantly increased. As a contrast, the variations of Tiefeng 40 were almost little under K-deficiency condition, which indicated tolerance to K+ deficiency variety could maintain higher efficient photosynthesis. On the 25th d after treatment, the minimal fluorescence (F0) of GD8521 was significantly increased and the maximal fluorescence (Fm), the maximum quantum efficiency of PSII photochemistry (Fv/ Fm), actual photochemical efficiency of PSII (ΦPSII), photochemical quenching (qP), and electron transport rate of PSII (ETR) were significantly decreased under K+ deficiency condition. In addition, the Rubisco content of GD8521 was significantly decreased in leaves. It is particularly noteworthy that the chlorophyll fluorescence parameters and Rubisco content of Tiefeng 40 were unaffected under K+ deficiency condition. On the other hand, the non-photochemical quenching (qN) of Tiefeng 40 was significantly increased. The dry matter weight of Tiefeng 40 was little affected under K+ deficiency condition. Results indicated that Tiefeng 40 could avoid or relieve the destruction of PSII caused by exceeded absorbed solar energy under K-deficiency condition and maintain natural photosynthesis and plant growth. It was an essential physiological mechanism for low-K-tolerant soybean under K-deficiency stress.
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5. Comparison and Analysis of QTLs, Epistatic Effects and QTL×Environment Interactions for Yield Traits Using DH and RILs Populations in Rice
ZHAO Xin-hua, QIN Yang, JIA Bao-yan, Suk-Man Kim, Hyun-Suk Lee, Moo-Young Eun, Kyung-Min
Journal of Integrative Agriculture    2013, 12 (2): 198-208.   DOI: 10.1016/S2095-3119(13)60219-1
摘要1520)      PDF    收藏
Two genetic linkage maps, constructed by DH and RILs populations derived from the same parents, were carried out for the identification and comparison of QTLs controlling yield traits across different years in rice (Oryza sativa L.). A total of 194 SSR and STS markers were used in two maps, of which 114 markers were same. The distribution of Samgang allele was higher in RILs population than it in DH population. Comparing with DH population, RILs population has more lines with higher yield and wider phenotypic transgressive segression for yield traits. Although most of QTLs for the same trait were different in two populations across different years, 8 QTLs (including gwp11.1, spp5.1, spp10.1, spp11.2, ssr1.1, ssr11.1, tgw9.1 and tgw11.1) were detected over 2 yr. It is important to note that ppp10.1, spp10.1 and tgw9.1 were identified in two populations, while spp10.1 and tgw9.1 were simultaneity observed across different years. Epistatic effects were more important than additive effects for PPP, SPP, yield in DH population and TGW, yield in RILs population. Epistatic effects of DH and RILs populations were different on the same genetic background in the present study, which illuminated the QE interaction played an important role on epistatic effect. Identification and comparison of QTLs for yield traits in DH and RILs populations should provide various and more precise information. The QTLs identified in present study would be valuable in marker-assisted selection program for improving rice yield.
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6. Relationship Between the N Concentration of the Leaf Subtending Boll and the Cotton Fiber Quality
WANG You-hua, ZHAO Xin-hua, CHEN Bing-lin, GAO Xiang-bin , ZHOU Zhi-guo
Journal of Integrative Agriculture    2012, 12 (12): 2013-2019.   DOI: 10.1016/S1671-2927(00)8738
摘要1089)      PDF    收藏
This experiments were conducted in Nanjing (118º50´E, 32º02´N) and Xuzhou (117°11´E, 34°15´N), Jiangsu Province, China, to study the response of fiber quality to the N concentration of the leaf subtending boll in cotton (Gossypium hirsutum L.). Results suggested that the N dilution curve of the leaf subtending boll can accurately indicate the stagespecific plant N status for fiber development. Fiber strength is likely to be the most variable fiber quality index responding to the leaf N variation which is different in cultivars. Fiber length was the most stable index among strength, length, micronaire, and elongation. There existed an optimum leaf N concentration for fiber strength development in each stage. The optimum leaf N regression curve was very close to the N dilution curve in the middle positional fruiting branches under the 240 kg N ha-1 soil N application rate.
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