厘清大豆种质籽粒中的类胡萝卜素组分和含量对大豆种质营养品质评价具有重要意义。本研究针对遗传多样性丰富的中国大豆种质的类胡萝卜素和叶绿素含量进行了系统分析，并揭示了不同营养品质组分间的相关性。结果显示基因型、种植年份、种质类型、子叶色和生态区来源显著影响籽粒中类胡萝卜素和叶绿素含量，其平均类胡萝卜素总含量变化范围为8.15-14.72 µg g^-1。大豆农家种的类胡萝卜素含量是栽培种的1.2倍，特别是绿子叶种质的类胡萝卜素和叶绿素含量显著高于黄子叶种质。需要指出的是单一组分中叶黄素的含量是最高的，其变化范围为1.35-37.44 µg g^-1。类胡萝卜素和叶绿素含量与其他品质组分显著相关，这有利于育种者在强化类胡萝卜素含量同时而不影响其他品质组分。我们结果证明了大豆籽粒中的类胡萝卜素含量是丰富的，但其积累宜受遗传因素、种质类型和种质来源的显著影响。我们还鉴定了一批高类胡萝卜素含量的大豆新种质，可以为大豆育种、食品加工和化妆品行业利用。

Isoprenoids are a functionally and structurally diverse class of natural organic chemicals. The universal precursors of all isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate are synthesized through the mevalonate and 2C-methyl- D-erythritol 4-phosphate (MEP) pathways, respectively. Many isoprenoids produced through the MEP pathway play an important role in plant acclimation to different light environments. Eupatorium adenophorum, an invasive weed in China, presents a remarkable capacity to acclimate to various light environments, which constitutes its solid foundation of being a successful invasive species. Thus we aimed at gaining a deeper insight into the regulation of MEP pathway in E. adenophorum to further understand the invasive mechanism. 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF or MCS) is an essential enzyme in the MEP pathway. In this paper, a novel IspF gene was cloned and characterized from E. adenophorum. Tissue-specific expression assays revealed a higher expression of EaIspF1 in leaves than in stems and roots. The expression of EaIspF1 was responsive to different light conditions. Some up-regulation of EaIspF1 expression was also found after the treatments with signal compounds and after wounding stress. Interestingly, the over-expression of EaIspF1 in Arabidopsis led to increase carotenoids contents, resulting in an enhanced tolerance to high light. Taken together, these results indicate that the EaIspF1-derived enzyme participates in isoprenoid metabolism and among others, the expression of this gene in E. adenophorum is involved in the regulation of plastidial isoprenoids, which play an important role in acclimation to various light environments.