Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 1024-1035.DOI: 10.1016/S2095-3119(13)60519-5

• 论文 • 上一篇    下一篇

EaIspF1, Essential Enzyme in Isoprenoid Biosynthesis from Eupatorium adenophorum, Reveals a Novel Role in Light Acclimation

 ZHANG Sheng-rui, JIANG Xue, WANG Ping, WU Di, WANG Qing-hua , HOU Yu-xia   

  1. College of Science, China Agricultural University, Beijing 100193, P.R.China
  • 收稿日期:2013-03-19 出版日期:2014-05-01 发布日期:2014-05-06
  • 通讯作者: HOU Yu-xia, Tel/Fax: +86-10-62733824, E-mail: houyuxia@tsinghua.org.cn
  • 作者简介:ZHANG Sheng-rui, E-mail: Shengruizhang@cau.edu.cn
  • 基金资助:

    This work was supported by a Public-Agricultural Research Project, Ministry of Agriculture, China (201103027) and the Genetically Modified Organism Breeding Major Project, Ministry of Agriculture, China (2013ZX08005002).

EaIspF1, Essential Enzyme in Isoprenoid Biosynthesis from Eupatorium adenophorum, Reveals a Novel Role in Light Acclimation

 ZHANG Sheng-rui, JIANG Xue, WANG Ping, WU Di, WANG Qing-hua , HOU Yu-xia   

  1. College of Science, China Agricultural University, Beijing 100193, P.R.China
  • Received:2013-03-19 Online:2014-05-01 Published:2014-05-06
  • Contact: HOU Yu-xia, Tel/Fax: +86-10-62733824, E-mail: houyuxia@tsinghua.org.cn
  • About author:ZHANG Sheng-rui, E-mail: Shengruizhang@cau.edu.cn
  • Supported by:

    This work was supported by a Public-Agricultural Research Project, Ministry of Agriculture, China (201103027) and the Genetically Modified Organism Breeding Major Project, Ministry of Agriculture, China (2013ZX08005002).

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摘要： Isoprenoids are a functionally and structurally diverse class of natural organic chemicals. The universal precursors of all isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate are synthesized through the mevalonate and 2C-methyl- D-erythritol 4-phosphate (MEP) pathways, respectively. Many isoprenoids produced through the MEP pathway play an important role in plant acclimation to different light environments. Eupatorium adenophorum, an invasive weed in China, presents a remarkable capacity to acclimate to various light environments, which constitutes its solid foundation of being a successful invasive species. Thus we aimed at gaining a deeper insight into the regulation of MEP pathway in E. adenophorum to further understand the invasive mechanism. 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF or MCS) is an essential enzyme in the MEP pathway. In this paper, a novel IspF gene was cloned and characterized from E. adenophorum. Tissue-specific expression assays revealed a higher expression of EaIspF1 in leaves than in stems and roots. The expression of EaIspF1 was responsive to different light conditions. Some up-regulation of EaIspF1 expression was also found after the treatments with signal compounds and after wounding stress. Interestingly, the over-expression of EaIspF1 in Arabidopsis led to increase carotenoids contents, resulting in an enhanced tolerance to high light. Taken together, these results indicate that the EaIspF1-derived enzyme participates in isoprenoid metabolism and among others, the expression of this gene in E. adenophorum is involved in the regulation of plastidial isoprenoids, which play an important role in acclimation to various light environments.

关键词: EaIspF1 , Eupatorium adenophorum , isoprenoid biosynthesis , light acclimation

Abstract: Isoprenoids are a functionally and structurally diverse class of natural organic chemicals. The universal precursors of all isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate are synthesized through the mevalonate and 2C-methyl- D-erythritol 4-phosphate (MEP) pathways, respectively. Many isoprenoids produced through the MEP pathway play an important role in plant acclimation to different light environments. Eupatorium adenophorum, an invasive weed in China, presents a remarkable capacity to acclimate to various light environments, which constitutes its solid foundation of being a successful invasive species. Thus we aimed at gaining a deeper insight into the regulation of MEP pathway in E. adenophorum to further understand the invasive mechanism. 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (IspF or MCS) is an essential enzyme in the MEP pathway. In this paper, a novel IspF gene was cloned and characterized from E. adenophorum. Tissue-specific expression assays revealed a higher expression of EaIspF1 in leaves than in stems and roots. The expression of EaIspF1 was responsive to different light conditions. Some up-regulation of EaIspF1 expression was also found after the treatments with signal compounds and after wounding stress. Interestingly, the over-expression of EaIspF1 in Arabidopsis led to increase carotenoids contents, resulting in an enhanced tolerance to high light. Taken together, these results indicate that the EaIspF1-derived enzyme participates in isoprenoid metabolism and among others, the expression of this gene in E. adenophorum is involved in the regulation of plastidial isoprenoids, which play an important role in acclimation to various light environments.

Key words: EaIspF1 , Eupatorium adenophorum , isoprenoid biosynthesis , light acclimation