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1. Effects of sediment load on the abrasion of soil aggregate and hydraulic parameters in experimental overland flow
WANG Jun-guang, YU Bing, NI Shi-min, GUO Zhong-lu, CAI Chong-fa
Journal of Integrative Agriculture    2020, 19 (4): 1117-1126.   DOI: 10.1016/S2095-3119(19)62719-X
摘要94)      PDF    收藏
The breakdown of soil aggregates under rainfall and their abrasion in overland flow are important processes in water erosion due to the production of more fine and transportable particles and, the subsequent significant effect on the erosion intensity.  Currently, little is known about the effects of sediment load on the soil aggregate abrasion and the relationship of this abrasion with some related hydraulic parameters.  Here, the potential effects of sediment load on soil aggregate abrasion and hydraulic parameters in overland flow were investigated through a series of experiments in a 3.8-m-long hydraulic flume at the slope gradients of 8.7 and 26.8%, unit flow discharges from 2×10–3 to 6×10–3 m2 s−1, and the sediment concentration from 0 to 110 kg m–3.  All the aggregates from Ultisols developed Quaternary red clay, Central China.  The results indicated that discharge had the most significant (P<0.01) effect on the aggregates abrasion with the contributions of 58.76 and 60.34%, followed by sediment feed rate, with contributions of 39.66 and 34.12% at the slope gradients of 8.7 and 26.8%, respectively.  The abrasion degree of aggregates was found to increase as a power function of the sediment concentration.  Meanwhile, the flow depth, friction factor, and shear stress increased as a power function along with the increase of sediment concentration at different slope gradients and discharges.  Reynolds number was obviously affected by sediment concentration and it decreased as sediment concentration increased.  The ratio of the residual weight to the initial weight of soil aggregates (Wr/Wi) was found to increase as the linear function with an increasing flow depth (P=0.008) or Reynolds number (P=0.002) in the sediment-laden flow.  The Wr/Wi values followed a power function decrease with increasing friction factor or shear stress in the sediment-laden flow, indicating that friction factor is the best hydraulic parameter for prediction of soil aggregate abrasion under different sediment load conditions.  The information regarding the soil aggregate abrasion under various sediment load conditions can facilitate soil process-based erosion modeling.
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2. The toxicological effect of dietary excess of saccharicterpenin, the extract of camellia seed meal, in piglets
WANG Man, YU Bing, HE Jun, YU Jie, LUO Yu-heng, LUO Jun-qiu, MAO Xiang-bin, CHEN Dai-wen
Journal of Integrative Agriculture    2020, 19 (1): 211-224.   DOI: 10.1016/S2095-3119(19)62789-9
摘要127)      PDF    收藏
Recently, saccharicterpenin extracted from Camellia oleifera seed meal has become a widely used feed supplement in animal husbandry.  In order to assess its safety, this study was designed to investigate the toxicity and histopathological effects of saccharicterpenin on piglets.  One hundred-fifty weaned pigs ((Yorkshire×Landrace)×Duroc), 75 males and 75 females with body weight (BW) of (7.35±0.29) kg, were randomly allotted to groups receiving diets supplemented with 0, 500, 1 000, 2 500 or 5 000 mg kg–1 saccharicterpenin for 70 d.  The diet with 500 mg kg–1 saccharicterpenin supplementation improved liver glutathione peroxidase (GSH-Px) activity, and the diet with 1 000 mg kg–1 saccharicterpenin supplementation improved liver glutathione S-transferase (GSH-S) activity in piglets on d 70 (P<0.05).  At 2 500 mg kg–1, saccharicterpenin in the diets reduced average daily feed intake (ADFI) and average daily gain (ADG) of piglets from d 1 to 35, damaged the cardiac tissue and liver on d 35, and decreased white blood cell counts (WBC), activities of catalase (CAT) and GSH-Px, and concentrations of glucose (GLU) and urea nitrogen (BUN) in the blood of piglets on d 70 (P<0.05).  In addition, diets with 5 000 mg kg–1 saccharicterpenin supplementation reduced ADFI, ADG and increased diarrhea rates of piglets from d 36 to 70, and decreased hemoglobin (HGB) concentration and activity of CAT in the blood of piglets on d 70 (P<0.05).  Moreover, at a rate of 5 000 mg kg–1, saccharicterpenin supplementation increased pancreas index on d 35 and hepatic index on d 70, and damaged cardiac tissue, liver and spleen during the whole experimental period (P<0.05).  These results suggested that dietary 500 mg kg–1 saccharicterpenin supplementation had beneficial effects on piglets, but excessive supplementation
(2 500 or 5 000 mg kg–1) of saccharicterpenin in the diets could lead to growth retardation, hematological abnormalities and organ injuries.
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3. Effect of dietary supplementation with mulberry (Morus alba L.) leaves on the growth performance, meat quality and antioxidative capacity of finishing pigs
ZENG Zhu, JIANG Jun-jie, YU Jie, MAO Xiang-bing, YU Bing, CHEN Dai-wen
Journal of Integrative Agriculture    2019, 18 (1): 143-151.   DOI: 10.1016/S2095-3119(18)62072-6
摘要311)      PDF    收藏
The present study was conducted to evaluate the effect of dietary mulberry (Morus alba L.) leaves powder (MLP) supplementation on meat quality of finishing pigs.  A total of 40 Duroc×Landrace×Yorkshire pigs (initial body weight of (40.5±0.63) kg) were randomly allotted into two treatments, fed either with control diet or 15% MLP diet for 85 d.  The results showed that MLP diet decreased (P≤0.05) average daily gain (ADG) and increased (P<0.05) feed/gain ratio (F/G) in the finishing and whole period.  MLP diet also decreased (P<0.05) carcass weight, dressing percentage, last rib and average backfat depth.  However, MLP diet increased (P<0.05) intramuscular fat (IMF) content, decreased (P<0.05) shear force, cooking loss and drip loss.  In addition, MLP diet increased (P<0.05) total antioxidative capacity, glutathione peroxidase and tended (P<0.10) to increase total superoxide dismutase in serum.  In longissimus thoracis, myosin heavy chain (MyHC) I and IIa mRNA levels were increased (P≤0.05) for MLP diet.  In conclusion, 15% MLP supplementation reduced the growth performance and carcass traits, but improved meat quality of finishing pigs possibly through the change of myofiber characteristics, enhancement of antioxidative capacity and increase of IMF. 
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4. Genome-wide identification, phylogeny and expression analysis of the SBP-box gene family in maize (Zea mays)
ZHANG Wei, LI Bei, YU Bin
Journal of Integrative Agriculture    2016, 15 (1): 29-41.   DOI: 10.1016/S2095-3119(14)60955-2
摘要2641)      PDF    收藏
The SQUAMOSA promoter binding protein (SBP)-box genes encode a kind of plant-specific transcription factors (TFs) and play important roles in the regulation of plant development. In this study, a genome-wide characterization of this family was conducted in maize (Zea mays). Thirty-one SBP-box genes were identified to be distributed in nine chromosomes and 16 of them were complementary to the mature ZmmiR156 sequences. All the Z. mays SBP (ZmSBP) genes were classified into two clusters with eight subgroups according to the phylogenetic analysis of proteins, which were consistent with the pattern of exon-intron structures. The phylogenetic tree of the ZmSBP, Oryza sativa SBP-like (OsSPL) and Arabidopsis thaliana SBP-like (AtSPL) genes were constructed and all the SBP-box genes were divided into eight groups, which was the same as the classification of ZmSBP genes. The comparision of the expression profiles of all SBP-box genes in these three species indicated that most orthologous genes had similar expression patterns. The results from this study provided a basic understanding of the ZmSBP genes and might facilitate future researches for elucidating the SBP-box genes function in maize.
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5. Effect of Dietary Folic Acid Supplementation on Growth Performance and Hepatic Protein Metabolism in Early-Weaned Intrauterine Growth Retardation Piglets
YAO Ying, YU Bing, CHEN Dai-wen, TIAN Gang, MAO Xiang-bing, ZHENG Ping, LIUJing-bo
Journal of Integrative Agriculture    2013, 12 (5): 862-868.   DOI: 10.1016/S2095-3119(13)60262-2
摘要1565)      PDF    收藏
To investigate the effects of dietary supplementation with folic acid on growth performance, hepatic protein metabolism and serum biochemical indices of early-weaned intrauterine growth retardation (IUGR) piglets, 24 male (Duroc× (Landrace×Yorkshire)) weaned (14-d-old) IUGR piglets were randomly divided into 3 treatments with 8 replicates of 1 piglet per replicate. The piglets in each treatment were fed basal diet supplementation with either 0 (control), 5 and 10 mg kg-1 folic acid. The trial lasted for 21 d. Dietary folic acid supplementation reduced average daily feed intake (ADFI) (P<0.05). In addition, the average daily gain (ADG) in 10 mg kg-1 folic acid group was significantly decreased (P<0.01) and the ratio of feed:gain (F/G) increased slightly (P>0.05). Serum folic acid concentration increased (P<0.01) with increasing folic acid inclusion, however, serum homocysteine concentration decreased significantly (P<0.01). Enhanced serum urine nitrogen (SUN) and diminished serum total protein (TP) as well as liver TP content were observed in 10 mg kg-1 folic acid group (P<0.05). Furthermore, the relative mRNA expressions of insulin-like growth factor 1 (IGF-1) and mammalian target of rapamycin (m-TOR) in liver were respectively tended to reduce (P=0.06) and significantly downregulated (P<0.05) in 10 mg kg-1 group, in compared with 5 mg kg-1 group. However, when compared with control group, folic acid supplementation had no significant effect on the mRNA abundance of IGF-1 and m-TOR. The results indicated that supplementation with 10 mg kg-1 folic acid impaired growth performance and hepatic protein metabolism of early-weaned IUGR piglets while 5 mg kg-1 folic acid enriched diet exerted limited positive effects.
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6. Morphological, Biochemical and Genetic Analysis of a Brittle Stalk Mutant of Maize Inserted by Mutator
FU Xue-qian, FENG Jing, YU Bin, GAO You-jun, ZHENG Yong-lian, YUE Bing
Journal of Integrative Agriculture    2013, 12 (1): 12-18.   DOI: 10.1016/S2095-3119(13)60200-2
摘要1531)      PDF    收藏
Mutants on stalk strength are essential materials for the studies on the formation of plant cell wall. In this study, a brittle stalk mutant of maize, designated as Bk-x, was screened from a Mutator inserted mutant library. At the germination and early seedling stage, the mutant plants were indistinguishable from the normal ones. However, all of the plant organs were brittle after the 5th-leaf stage and remained brittle throughout the rest of the growing period. Microstructure observation showed that the cell wall in vascular bundle sheath of Bk-x was thinner than that in normal plants. The leaf mechanical strength in Bk-x was 77.9% of that in normal plants growing at Xishuangbanna (BN), Yunnan province and that was 61.7% in Wuhan (WH), Hubei Province, China. The proportion of cellulose was 12.3% in Bk-x, which was significantly lower than that in normal plants (26.7%), while the soluble sugar content was 36.1% in Bk-x, which is significantly higher than that in normal plants (12.4%). Genetic analysis using two F2 populations and one F2:3 families demonstrated that the trait of brittle stalk is controlled by a single recessive gene.
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