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1. Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum
HUANG Tian-yu, ZHANG Rui-bin, YANG Lu-lu, CAO Song, Frederic FRANCIS, WANG Bing, WANG Gui-rong
Journal of Integrative Agriculture    2022, 21 (5): 1414-1423.   DOI: 10.1016/S2095-3119(20)63577-8
摘要236)      PDF    收藏

本研究通过系统发育分析鉴定了蚜虫中一个保守的气味受体分支,并对这一支中豌豆蚜气味受体ApisOr23的功能进行了研究。序列分析的结果显示,豌豆蚜、大豆蚜和棉蚜的同源受体Or23序列相似性为94.28%。此外,我们对8种蚜虫同源受体Or23序列进行保守基序 (motif) 分析,进一步验证了Or23分支的高度保守性。组织特异性表达分析结果显示,豌豆蚜ApisOr23主要在触角高表达。随后,我们利用非洲爪蟾卵母细胞表达系统对ApisOr23进行体外功能研究,结果显示在所有测试的化合物中,ApisOr23对反-2-己烯醛、顺-2-己烯醇、庚醇、4’-乙基苯乙酮和乙酸乙酯这5种植物挥发物有明显的电生理活性。其中,反-2-己烯醛作为豆科植物的一种主要挥发物,能够最大程度地激活ApisOr23。本研究揭示了蚜虫中保守的气味受体Or23的功能,推测该受体在蚜虫寄主识别过程中起到重要作用。


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2. Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR
FU Yan-feng, FU Jin-luan, YANG Lu, TIAN Ming-ming, CHEN Wen-cheng , WANG Ai-guo
Journal of Integrative Agriculture    2011, 10 (9): 1445-1451.   DOI: 10.1016/S1671-2927(11)60137-5
摘要1724)      PDF    收藏
Erythropoietin-producing hepatocellular receptor and its membrane-bound ligands (Eph-Ephrin) system could regulatesome mammalian blastocyst attachment and spreading. In order to investigate the involvement of the Eph-Ephrin systemin swine embryo attachment, mRNA expression of Eph-Ephrin molecules in endometrium was examined by real-time RTPCRduring embryo implantation in pigs. The results indicated that mRNA expressions of Eph A5, A7 and Ephrin A5 allcontinually increased from pregnancy day 13 to 24. Ephrin A3 mRNA expression significantly increased from day 13 to 18and decreased from day 18 to 24, and the expression was the lowest on pregnancy day 13 and the highest on day 18.However, Ephrin A4 mRNA expression was the lowest on pregnancy day 18 and the highest on day 24, and the expressiondecreased from day 13 to 18 and increased from day 18 to 24. Furthermore, mRNA expressions of Eph A5 and A7 were bothfound in other tissues, such as brain, muscle, intestine, stomach, etc. These findings suggest that the Eph-Ephrin systemmay play an important role in regulating the contact between blastocysts and endometrium during swine embryoimplantation.
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