Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum

doi:10.1016/S2095-3119(20)63577-8

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (5): 1414-1423.DOI: 10.1016/S2095-3119(20)63577-8

收稿日期:2020-08-19 接受日期:2020-11-18 出版日期:2022-05-01 发布日期:2020-11-18

Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum

HUANG Tian-yu^{1, 3*}, ZHANG Rui-bin^{1, 2*}, YANG Lu-lu¹, CAO Song¹, Frederic FRANCIS³, WANG Bing¹, WANG Gui-rong^{1, 2}

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
²Guangdong Laboratory for Lingnan Modern Agriculture (Shenzhen Branch), Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
³ Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux 5030, Belgium

Received:2020-08-19 Accepted:2020-11-18 Online:2022-05-01 Published:2020-11-18
About author:HUANG Tian-yu, E-mail: huangty_caas@hotmail.com; ZHANG Rui-bin, E-mail: zhangruibin1990@126.com; Correspondence WANG Gui-rong, E-mail: wangguirong@caas.cn; WANG Bing, E-mail: bwang@ippcaas.cn; Frederic FRANCIS, E-mail: frederic.francis@uliege.be * These authors contributed equally to this study.
Supported by:
This work was funded by the National Natural Science Foundation of China (31572072 and 31725023), the Intergovernmental International Science, Technology and Innovation Cooperation Key Project, China (2019YFE0105800), and the Shenzhen Science and Technology Program, China (KQTD20180411143628272). The funders had no role in study design, data collection or analysis, decision to publish or preparation of the manuscript.

摘要/Abstract

摘要：

本研究通过系统发育分析鉴定了蚜虫中一个保守的气味受体分支，并对这一支中豌豆蚜气味受体ApisOr23的功能进行了研究。序列分析的结果显示，豌豆蚜、大豆蚜和棉蚜的同源受体Or23序列相似性为94.28%。此外，我们对8种蚜虫同源受体Or23序列进行保守基序 (motif) 分析，进一步验证了Or23分支的高度保守性。组织特异性表达分析结果显示，豌豆蚜ApisOr23主要在触角高表达。随后，我们利用非洲爪蟾卵母细胞表达系统对ApisOr23进行体外功能研究，结果显示在所有测试的化合物中，ApisOr23对反-2-己烯醛、顺-2-己烯醇、庚醇、4’-乙基苯乙酮和乙酸乙酯这5种植物挥发物有明显的电生理活性。其中，反-2-己烯醛作为豆科植物的一种主要挥发物，能够最大程度地激活ApisOr23。本研究揭示了蚜虫中保守的气味受体Or23的功能，推测该受体在蚜虫寄主识别过程中起到重要作用。

Abstract: Pea aphid, Acyrthosiphon pisum, is a serious pest of many different leguminous plants, and it mainly relies on its odorant receptors (Ors) to discriminate among host species. However, less is known about the role that Ors play in the host plant location. In this study, we identified a novel conserved odorant receptor clade by phylogenetic analysis, and conducted the functional analysis of ApisOr23 in A. pisum. The results showed that the homologous Ors from A. pisum, Aphis glycines and Aphis gossypii share 94.28% identity in amino acid sequences. Moreover, conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species, providing further proof of the high conservation level of the Or23 clade. According to the tissue expression pattern analysis, ApisOr23 was mainly expressed in the antennae. Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles, namely trans-2-hexen-1-al, cis-2-hexen-1-ol, 1-heptanol, 4´-ethylacetophenone, and hexyl acetate. Among them, trans-2-hexen-1-al, which is one of the main volatile organic compounds released from legume plants, activated the highest response of ApisOr23. Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.

Key words: Acyrthosiphon pisum , odorant receptor , phylogenetic analysis , two-electrode voltage clamp , trans-2-hexen-1-al

. [J]. Journal of Integrative Agriculture, 2022, 21(5): 1414-1423.

HUANG Tian-yu, ZHANG Rui-bin, YANG Lu-lu, CAO Song, Frederic FRANCIS, WANG Bing, WANG Gui-rong. Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum[J]. Journal of Integrative Agriculture, 2022, 21(5): 1414-1423.

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Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum

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