本研究通过室内试管石英砂培养的方法，测试香蕉穿孔线虫对本氏烟草的侵染致病过程和规律，明确了香蕉穿孔线虫侵染本氏烟草根系主要在根系皮层寄生，导致皮层细胞分解、组织腐烂。通过采用不同致病型和不同寄主来源的香蕉穿孔线虫群体对本氏烟草侵染致病的接种条件进行测定，以及对发病植株的症状表现、受害严重度和线虫繁殖率等致病性相关因子的评估，确定了香蕉穿孔线虫与本氏烟草互作研究的最佳测试条件和结果评估标准，从而建立了本氏烟草作为寄主的香蕉穿孔线虫致病性测定方法：（1）在玻璃培养试管中装入约占高度1/3 干燥的石英砂并灭菌两次；（2）将20天苗龄的本氏烟草幼苗移栽到试管中，在25±1℃条件下培养定植10 天；（3）以150条/株的接种虫量将香蕉穿孔线虫的雌虫接种到烟草根际；（4）在接种线虫30天后检测植株发病严重度、生长量和线虫繁殖量。本研究还采用该方法对致病型和寄主来源不同的8个香蕉穿孔线虫种群的致病性强弱进行了测定，证明了该方法的可行性。本研究结果为将本氏烟草作为香蕉穿孔线虫与植物互作研究的模式植物提供了依据和方法，为香蕉穿孔线虫致病性及其与寄主互作分子机制研究提供了新途径，对利用本氏烟草作为模式寄主研究香蕉穿孔线虫的致病机制和防治方法具有重要意义。

Ten populations of Radopholus similis from different ornamental hosts were tested for their parasitism and pathogenicity to water spinach (Ipomoea aquatic), malabar spinach (Basella rubra), and squash (Cucurbita moschata) in pots. The results showed all three plants were new hosts of R. similis. Growth parameters of plants inoculated with nematodes were significantly lower than those of healthy control plants. All R. similis populations were pathogenic to the three plants, but pathogenicity differed among populations from different hosts. The same R. similis populations also showed different pathogenic effects in the three different plants. RadN5 population from Anthurium andraeanum had the highest pathogenicity to the three studied plants. RadN1 from A. andraeanum had the lowest pathogenicity to squash and RadN7 from Chrysalidocarpus lutesens had the lowest pathogenicity to water spinach and malabar spinach. R. similis is usually associated with root tissues, but here we report that it could be found to move and feed in the stem bases of all three studied plants. Sequence and phylogenetic analyses of DNA markers of the 18S rRNA, 28S rRNA, ITS rRNA, and mitochondrial DNA gene sequences of ten R. similis populations revealed significant genetic diversity. RadN5 and RadN6 populations from anthurium showed a close genetic relationship and could be distinguished from other populations by PCR-RFLP. At the same time, RadN5 and RadN6 populations were the most pathogenic to three studied plants. These results confirm the existence of large biological variability and molecular diversity among R. similis populations from the same or different hosts, and these characteristics are related to pathogenic variability.