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1. 硝铵混合供氮增强氮素吸收和同化能力是促进苗期玉米生长的驱动力
WANG Peng, WANG Cheng-dong, WANG Xiao-lin, WU Yuan-hua, ZHANG Yan, SUN Yan-guo, SHI Yi, MI Guo-hua
Journal of Integrative Agriculture    2023, 22 (6): 1896-1908.   DOI: 10.1016/j.jia.2023.04.037
摘要202)      PDF    收藏
与单独供硝(NO3-)或者单独供铵(NH4+)相比,混合供氮能够促进苗期玉米的生长。前期研究表明,混合供氮不仅可以提高玉米的光合效率,还可以促进地上部生长素的合成来增强叶片生长,进而为碳和氮的利用构建一个较大的库。然而,该过程是否依赖于氮的吸收还尚不清楚。在此,将玉米幼苗在具有三种供氮形态(单独供硝,75/25硝铵比和单独供铵)的水培实验中进行生长。结果表明,在0-3天,混合供氮下玉米生长速率和地上部含氮量与单独供硝处理间无显著差异,在6-9天,混合供氮下玉米生长速率和地上部含氮量显著高于单独供硝处理。于此同时,虽然混合供氮条件下15NO3-与15NH4+的瞬时吸收速率较单独供硝和单独供铵相比皆有所下降,但混合供氮在6-9天具有最高的总氮吸收速率。QRT-PCR结果表明,长期混合供氮条件下根系N吸收的增加可能与长期处理下NO3-转运蛋白基因(例如ZmNRT1.1AZmNRT1.1BZmNRT1.1CZmNRT1.2ZmNRT1.3)的高表达或铵转运蛋白基因(例如ZmAMT1.1A)的高表达有关,尤其是后者。此外,与单独供硝处理相比,混合供氮处理下植株地上部与根系中具有较高的谷氨酰胺合酶(GS)活性以及氨基酸含量。硝酸还原酶酶(NR)和GS酶抑制剂实验进一步证明了混合供氮情况下氮的同化能力对于玉米生长促进是至关重要的。该研究证明了混合供氮能够促进氮素吸收并进一步促进了氮的同化,而该过程可能是促进玉米上生长的主要驱动力。
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2. Sequencing and Phylogenetic Analysis of Potato virus Y Liaoning Isolate in China
WANG Fang, GAO Zheng-liang, AN Meng-nan, ZHOU Ben-guo , WU Yuan-hua
Journal of Integrative Agriculture    2013, 12 (7): 1195-1200.   DOI: 10.1016/S2095-3119(13)60442-6
摘要1657)      PDF    收藏
Complete genome sequence of Potato virus Y Liaoning isolate (PVY-LN) causing tobacco vein necrosis symptoms were isolated from Liaoning Province in China. Genome sequences of PVY-LN was 9 714 nucleotides in length, excluding the 3´- terminal poly (A) tail. PVY-LN encodes a single long open reading frame (ORF) of polyprotein that is predicted to be cleaved into ten mature proteins by three viral proteases. No recombination can be predicted in PVY-LN sequences compared with that of the other PVY strains using Recombination Detection Programe v. 4.16 (RDP4). Complete genome sequence comparison and phylogenetic analysis indicated that PVY-LN is closely related to PVY necrosis strain (PVYN).
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3. Genomic Analysis of the Natural Population of Wheat dwarf virus inWheat fromChina and Hungary
LIU Yan, WANG Biao, Gyula Vida, Mónika Cséplo-Károlyi, WU Bei-lei, WU Yuan-hua, WANG Xifeng
Journal of Integrative Agriculture    2012, 12 (12): 2020-2027.   DOI: 10.1016/S1671-2927(00)8739
摘要1399)      PDF    收藏
During the last decade, the leafhopper transmitted Wheat dwarf virus (WDV) has become a serious problem both in northwestern China and Hungary. In order to study the molecular diversity and population structure of WDV in these two countries, 39 Chinese isolates and 16 Hungarian isolates were collected from different regions of China and Hungary, and their genomes were sequenced. All isolates belonged to the wheat strain of WDV and showed limited genetic diversity. The highest and lowest nucleotide sequence identities among isolates from China and Hungary were 99.9 and 90%, respectively. In all isolates, the lowest nucleotide sequence identity was 89.5% between MO10-1 and KP10-5, which were collected from Martonvásár and Kompolt, Hungary. Phylogenetic analyses showed the genome sequences of 55 WDV isolates belong to two big clades, but no clear correlation to geographical location. Population difference analyses indicated that the Chinese and Hungarian WDV populations have no significant difference. The regions in WDV genome with relatively low nucleotide diversities represented protein coding regions suggested that these regions evolved under negative selection, and might be one of the causes restricting the number of genetic variants.
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