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1. 华东南地区机械化栽培下头季稻氮肥的优化管理及其对再生稻的递延效应
HUANG Jin-wen, WU Jia-yi, CHEN Hong-fei, ZHANG Zhi-xing, FANG Chang-xun, SHAO Cai-hong, LIN Wei-wei, WENG Pei-ying, Muhammad Umar KHAN, LIN Wen-xiong
Journal of Integrative Agriculture    2022, 21 (2): 351-364.   DOI: 10.1016/S2095-3119(21)63668-7
摘要255)      PDF    收藏

本试验以甬优1540”为材料,通过2年的田间重复试验,在季稻总施纯氮量(225.00kg·hm-2)不变的前提下,设置季不同氮肥运筹处理(基肥:第一次分蘖肥:第二次分蘖肥:穗肥比分别为3:1:2:4(N1)3:2:1:4(N2)3:3:0:4(N3)4:3:0:3(N4)),从水稻群体分蘖特性、冠层结构特点、光合生理变化及干物质积累与运转规律入手,探讨了东南稻作区机械化栽培下季稻氮肥运筹对再生稻产量形成的影响。结果表明,季前氮后移施肥的N1处理有助于增加季分蘖数,提高分蘖成穗率,N1处理成穗率高达70.18%,比基蘖肥比重高的当地常规施肥N4处理提高了9.15%N1处理还有助于提高群体LAI值,其季及再生季齐穗期LAI值分别比N4处理提高了16.52% 29.87%。就茎鞘物质运转率及冠层光能截获率而言,N1处理下其季稻茎鞘物质运转率比N4处理提高了50.57%,而其再生季齐穗期的冠层光能截获率比N4处理提高了5.07%在本试验中,头季稻前氮后移的N1处理两季实际总产量最高,2年平均实际总产达17351.23 kg·hm-2,比当地常规施肥N4处理提高了23.00%。由此可见,季稻合理氮肥处理有利其提高健桩存量,从而为再生季作物的群体及产量形成奠定基础。在本研究中,低留桩机械化栽培下季稻适当的前氮后移,不仅提高季稻产量,而且还有利其再生腋芽的萌发,增加再生季成穗率,从而实现水稻一种两收目的。

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2. Silicon impacts on soil microflora under Ralstonia Solanacearum inoculation
LIN Wei-peng, JIANG Ni-hao, PENG Li, FAN Xue-ying, GAO Yang, WANG Guo-ping, CAI Kun-zheng
Journal of Integrative Agriculture    2020, 19 (1): 251-264.   DOI: 10.1016/S2095-3119(18)62122-7
摘要148)      PDF    收藏
Silicon (Si) can increase plant resistance against bacterial wilt caused by Ralstonia solanacearum and enhance plant immune response.  However, whether Si alleviates soil-borne disease stress through altering soil microbial community component and diversity is not clear.  In this study, effects of Si application under R. solanacearum inoculation with or without plant on soil bacterial and fungal communities were investigated through high-throughput pyrosequencing technique.  The results showed that Si addition significantly reduced bacterial wilt incidence.  However, Si did not reduce the amount of R. solanacearum in rhizosphere soil.  Principal components analysis showed that soil microbial community composition was strongly influenced by Si addition.  Total 63.7% bacterial operational taxonomic units (OTUs) and 43.8% fungal OTUs were regulated by Si addition regardless of the presence of tomato plants, indicating the independent effects of Si on soil microbial community.  Si-added soil harbored a lower abundance of Fusarium, Pseudomonas, and Faecalibacterium.  Our finding further demonstrated that exogenous Si could significantly influence soil microbial community component, and this may provide additional insight into the mechanism of Si-enhanced plant resistance against soil-borne pathogens.
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3. Physiological response and phenolic metabolism in tomato (Solanum lycopersicum) mediated by silicon under Ralstonia solanacearum infection
FAN Xue-ying, LIN Wei-peng, LIU Rui, JIANG Ni-hao, CAI Kun-zheng
Journal of Integrative Agriculture    2018, 17 (10): 2160-2171.   DOI: 10.1016/S2095-3119(18)62036-2
摘要342)      PDF(pc) (878KB)(552)    收藏
Bacterial wilt, caused by Ralstonia solanacearum (Rs) is a serious soil-borne disease and silicon can enhance tomato resistance against this disease.  However, few studies have focused on the mechanisms of Si-mediated pathogen resistance from the rhizosphere perspective.  In this study, two tomato genotypes, HYT (susceptible) and H7996 (resistant), were used to investigate the effects of silicon application on disease inhibition, root growth, and organic acid content in both roots and root exudates under R. solanacearum infection.  The results showed that Si application significantly suppressed bacterial wilt in HYT, but had no effect in H7996.  Silicon concentrations in roots, stems and leaves of tomato were significantly increased by Si treatment under R. solanacearum inoculation.  In HYT, Si application increased root dry weight by 22.8–51.6% and leaf photosynthesis by 30.6–208.0%, and reduced the concentrations of citric acid in root exudates by 71.4% and in roots by 83.5%.  However, organic acids did not influence R. solanacearum growth.  Results also demonstrated that salicylic acid (SA) content in roots was significantly increased by silicon addition for H7996 and exogenous SA application could reduce bacterial wilt disease index.  Collectively, these results suggest that Si-modulated phenolic compound metabolism in roots or root exudates, especially citric acid and SA, may be a potential mechanism in the amelioration of bacterial wilt disease by Si.
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4. Relationship of chemical properties of different peanut varieties to peanut butter storage stability
GONG A-na, SHI Ai-min, LIU Hong-zhi, YU Hong-wei, LIU Li, LIN Wei-jing, WANG Qiang
Journal of Integrative Agriculture    2018, 17 (05): 1003-1010.   DOI: 10.1016/S2095-3119(18)61919-7
摘要671)      PDF    收藏
This study examined the effect of peanut quality on the storage stability of peanut butter.  The quality of 17 varieties of peanuts was analyzed, and each was used to prepare peanut butter.  For different storage temperatures and durations, stability of the peanut butter was measured according to three indicators: peroxide value, acid value, and centrifugal rate.  The correlation between peanut components and peanut butter storage stability was also investigated.  The results indicated significant differences in fatty acid composition between different varieties of peanut.  Peanut butter prepared with high oleic peanuts (Kainong 17-15) had a significantly longer shelf life than that of other varieties.  The significant correlation between the stability of peanut butter and peanut quality suggests that oleic acid and linoleic acid were the main influencing factors on stability.  This study finds that the high oleic peanuts (HOP) is the most suitable variety for making peanut butter, which can allow farmers and processors to choose the specific variety for better product and shelf life. 
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5. Impacts of chemical fertilizer reduction and organic amendments supplementation on soil nutrient, enzyme activity and heavy metal content
NING Chuan-chuan, GAO Peng-dong, WANG Bing-qing, LIN Wei-peng, JIANG Ni-hao, CAI Kun-zheng
Journal of Integrative Agriculture    2017, 16 (08): 1819-1831.   DOI: 10.1016/S2095-3119(16)61476-4
摘要745)      PDF    收藏
    Excessive use of agro-chemicals (such as mineral fertilizers) poses potential risks to soil quality. Application of organic amendments and reduction of inorganic fertilizer are economically feasible and environmentally sound approaches to develop sustainable agriculture. This study investigated and evaluated the effects of mineral fertilizer reduction and partial substitution of organic amendment on soil fertility and heavy metal content in a 10-season continually planted vegetable field during 2009–2012. The experiment included four treatments: 100% chemical fertilizer (CF100), 80% chemical fertilizer (CF80), 60% chemical fertilizer and 20% organic fertilizer (CF60+OM20), and 40% chemical fertilizer and 40% organic fertilizer (CF40+OM40). Soil nutrients, enzyme activity and heavy metal content were determined. The results showed that single chemical fertilizer reduction (CF80) had no significant effect on soil organic matter content, soil catalase activity and soil heavy metal content, but slightly reduced soil available N, P, K, and soil urease activity, and significantly reduced soil acid phosphatase activity. Compared with CF100, 40 or 60% reduction of chemical fertilizer supplemented with organic fertilizer (CF60+OM20, CF40+OM40) significantly increased soil organic matter, soil catalase activity and urease activity especially in last several seasons, but reduced soil available P, K, and soil acid phosphatase activity. In addition, continuous application of organic fertilizer resulted in higher accumulation of Zn, Cd, and Cr in soil in the late stage of experiment, which may induce adverse effects on soil health and food safety.
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6. Identification of Antifungal Substance (Iturin A2) Produced by Bacillus subtilis B47 and Its Effect on Southern Corn Leaf Blight
YE Yun-feng, LI Qi-qin, FU Gang, YUAN Gao-qing, MIAO Jian-hua , LIN Wei
Journal of Integrative Agriculture    2012, 12 (1): 90-99.   DOI: 10.1016/S1671-2927(00)8509
摘要2084)      PDF    收藏
Bacillus subtilis B47 is an endophytic bacterium of tomato, and produces substance that strongly inhibits the growth of Bipolaris maydis, the pathogen of southern corn leaf blight (SCLB), as well as several other phytopathogenic fungi. The antifungal substance was purified from the broth culture of the bacterium using acid precipitation, methanol extraction, and three-step chromatography. Based on FT-IR spectrometry, amino acid composition, and MALDI-TOF-MS/MS CID analyses, the antifungal substance was identified as iturin A2, a cyclic lipopeptide antibiotic. To evaluate the efficacy of iturin A2 for control of SCLB, partially purified iturin A2 (75%, w/v) was applied under different conditions. At the concentration of 300 mg kg-1, iturin A2 showed efficacy ranging from 100 to 53.1% under in vitro, in plot and in field conditions. This efficacy was higher than or similar to that of the fungicide chlorothalonil. When the concentration of iturin A2 was increased to 500 mg kg-1, the control efficacy was enhanced to 64.2% in field, which was significantly higher than that of chlorothalonil. These results indicate that iturin A2 has potential for SCLB control and could be a substitute to synthetic fungicides. To our knowledge, this is the first report on using partially purified iturin A for control of SCLB under field conditions.
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7. 烟酰胺单核苷酸(NMN)赋予植物广谱抗病性
Shuangxi Zhang, Xinlin Wei, Rongbo Wang, Hejing Shen, Hehuan You, Langjun Cui, Yi Qiang, Peiqing Liu, Meixiang Zhang, Yuyan An
Journal of Integrative Agriculture    DOI: 10.1016/j.jia.2024.04.027
录用日期: 2024-05-23

8. 利用促生枯草芽孢杆菌表达病原相关分子模式:一种同时提高植物免疫和生长的有效策略
Shuangxi Zhang, Xinlin Wei, Hejing Shen, Qinhu Wang, Yi Qiang, Langjun Cui, Hongxing Xu, Yuyan An, Meixiang Zhang
Journal of Integrative Agriculture    DOI: 10.1016/j.jia.2024.04.034
录用日期: 2024-05-24