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1. Environmental risks for application of magnesium slag to soils in China
WANG Xiao-bin, YAN Xiang, LI Xiu-ying
Journal of Integrative Agriculture    2020, 19 (7): 1671-1679.   DOI: 10.1016/S2095-3119(19)62835-2
摘要141)      PDF    收藏
Magnesium slag (MS) is one of the main industrial solid wastes produced by the magnesium industry.  Solving the problem of its disposal has attracted much attention with increasing amounts of solid wastes generated in the production of metallic magnesium.  Because MS contains calcium (Ca), magnesium (Mg), and silicon (Si), some have tried to use MS as Si-Ca-Mg fertilizer or for soil amendment in agriculture.  However, in the magnesium metallurgical process, some pollutant elements are introduced into MS, resulting in the enrichment of these pollutants in MS, such as arsenic (As), chromium (Cr), cadmium (Cd), mercury (Hg), copper (Cu), nickel (Ni), fluorine (F) and chlorine (Cl).  Research indicates that the enrichment of these pollutants can result in high levels, especially for Cd, Hg, Cu, Ni, F and Cl (0–4, 0–0.74, 20–127, 100–170, 2 277–14 800 and 133–1 000 mg kg–1, respectively) in some MS in China.  These levels are often far beyond the limits (≤0.3, ≤0.5, ≤50 and ≤60 mg kg–1 for Cd, Hg, Cu and Ni, respectively) of the Chinese Risk Screening Values for Soil Contamination of Agricultural Land based on the Soil Environmental Quality Risk Control Standard for Soil Contamination of Agricultural Land (GB 15618-2018) or the critical reference values (≤800 and ≤200 for F and Cl, respectively).  The elements Hg, Cu, Cr and F (detected in MS leachate at 0.00023–0.0052, 0.043–3.89, 0.026–0.171, and 1.43–8.52 mg L–1, respectively) also exceed the limits (Class IV–V) of the Chinese Standard for Groundwater Quality (GB/T 14848-2017).  Based on the above results, it is suggested that without any pretreatment for reducing harmful pollutants MS should not be allowed to be applied for soil remediation or conditioning directly into farmlands in order to ensure soil health, food safety and environmental quality. 
 
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2. Environmental risk for application of ammonia-soda white mud in soils in China
WANG Xiao-bin, YAN Xiang, LI Xiu-ying
Journal of Integrative Agriculture    2020, 19 (3): 601-611.   DOI: 10.1016/S2095-3119(19)62745-0
摘要153)      PDF    收藏
In recent years, some reports, mainly from Chinese research, show that there has been an increasing trend in the use of ammonia-soda residue (ASR) (or called ammonia-soda white mud) as a soil conditioner in farmlands.  Up to now, the studies on ASR have focused on its utilization for acid soil amendment in agriculture, but few studies have assessed its environmental risk.  ASR contains pollutant elements such as mercury (Hg), cadmium (Cd), copper (Cu) and fluorine (F) and the purpose of this study was to review research on the environmental impacts of ASR application in agriculture.  Observations obtained from 23 research reports indicate that the concentrations of  Hg, Cd, Cu, F and Cl (0–170, 0.01–2.8, 4.5–200, 2000–24700 and 1 600–188 000 mg kg–1, respectively) in ASR may exceed the limits (≤0.5, ≤0.3 and ≤50 mg kg–1 for Hg, Cd and Cu, respectively) of Chinese Risk Screening Values for Soil Contamination of Agricultural Land (GB 15618-2018 2018) or the refereed critical value (≤800 and ≤200 mg kg–1 for F and Cl, respectively) based on Chinese research.  The concentrations of the elements Hg, Cd, Cu, F and Cl  in the leachate of ASR detected by the extraction tests also exceed the limits (Class IV–V) of the Chinese Standard for Groundwater Quality (GB/T 14848-2017 2017).  Based on the above results, it is suggested that ASR without any pretreatment for reducing harmful pollutants should not be used for soil remediation or conditioning of farmlands, to ensure soil health, food safety and environmental quality. 
 
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3. Acquisition of Insect-Resistant Transgenic Maize Harboring a Truncated cry1Ah Gene via Agrobacterium-Mediated Transformation
LI Xiu-ying, LANG Zhi-hong, ZHANG Jie, HE Kang-lai, ZHU Li , HUANG Da-fang
Journal of Integrative Agriculture    2014, 13 (5): 937-944.   DOI: 10.1016/S2095-3119(13)60531-6
摘要2075)      PDF    收藏
A novel insecticidal gene cry1Ah was cloned from Bacillus thuringiensis isolate BT8 previously for plant genetic engineering improvement. Truncated active Cry1Ah toxin has a toxicity level similar to that of the full-length Cry1Ah toxin. In this study, plant expression vector pMhGM harboring truncated cry1Ah gene was transformed into maize (Zea mays L.) immature embryos by Agrobacterium tumefaciens-mediated transformation at which maize alcohol dehydrogenase matrix attachment regions (madMARs) were incorporated on both sides of the gene expression cassette to improve gene expression. A total of 23 PCR positive events were obtained with a transformation efficiency of 5% around. Bioassay results showed that events 1-4 and 1-5 exhibited enhanced resistance to the Asian corn borer (Ostrinia furnacalis). These two events were further confirmed by molecular analysis. Southern blot suggested that a single copy of the cry1Ah gene was successfully integrated into the maize genome. Western blot and ELISA showed that the foreign gene cry1Ah was expressed stably at high level in maize and could be inherited stably over generations. The results of a bioassay of T1-T4 transgenic maize plants indicated that the transgenic plants were highly toxic to the Asian corn borer and their resistance could be inherited stably from generation to generation. Thus, events 1-4 and 1-5 are good candidates for the breeding of insect-resistant maize.
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