昆虫蜕皮激素由Halloween家族基因合成，在几个关键的发育事件中发挥重要作用，包括蜕皮和变态。然而，这些Halloween基因在莲草直胸跳甲中的功能作用仍不清楚。在本研究中，通过RT-qPCR技术检测了两个Halloween基因AhCYP307A2和AhCYP314A1在不同发育阶段的表达模式。此外，利用RNA干扰（RNAi）技术探究了这两个基因的功能，并通过对莲草直胸跳甲雌性卵巢进行解剖观察其卵巢发育情况。qPCR结果显示，AhCYP307A2和AhCYP314A1在末龄幼虫和雌虫中均显著高表达。此外，AhCYP307A2在卵期和蛹中也有高表达，但显著低于在末龄幼虫和雌虫中的表达水平。RNAi结果显示，注射dsAhCYP307A2和dsAhCYP314A1显著抑制了它们的表达以及三个相关的卵黄原蛋白基因AhVgs的转录水平。敲除AhCYP307A2和AhCYP314A1可显著抑制幼虫蜕皮，破坏末龄幼虫-蛹-成虫的转变，延迟卵巢发育，停止产卵。以上研究结果表明，AhCYP307A2和AhCYP314A1在莲草直胸跳甲末龄幼虫-蛹-成虫转换期的蜕皮发育和繁殖过程中发挥重要的调控作用。

Thermal adaptation plays a fundamental role in shaping the distribution and abundance of insects, and heat shock proteins (Hsps) play important roles in the temperature adaptation of various organisms. To better understand the temperature tolerance of the indigenous ZHJ2-biotype of whitefly Bemisia tabaci species complex, we obtained complete cDNA sequences for hsp90, hsp70, and hsp20 and analyzed their expression profiles under different high temperature treatments by real-time quantitative polymerase chain reaction. The high temperature tolerance of B. tabaci ZHJ2-biotype was determined by survival rate after exposure to different high temperatures for 1 h. The results showed that after 41°C heat-shock treatment for 1 h, the survival rates of ZHJ2 adults declined significantly and the estimated temperature required to cause 50% mortality (LT50) is 42.85°C for 1 h. Temperatures for onset (Ton) or maximal (Tmax) induction of hsps expression in B. tabaci ZHJ2-biotype were 35 and 39°C (or 41°C). Compared with previous studies, indigenous ZHJ2- biotype exhibits lower heat temperature stress tolerance and Ton (or Tmax) than the invasive B-biotype.