Journal of Integrative Agriculture ›› 2019, Vol. 18 ›› Issue (4): 900-906.DOI: 10.1016/S2095-3119(19)62591-8

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  • 收稿日期:2018-06-25 出版日期:2019-04-01 发布日期:2019-04-01

Relationship between copulation and cold hardiness in Ophraella communa (Coleoptera: Chrysomelidae)

ZHAO Chen-chen*, YUE Lei*, WANG Yao, GUO Jian-ying, ZHOU Zhong-shi, WAN Fang-hao
  

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • Received:2018-06-25 Online:2019-04-01 Published:2019-04-01
  • Contact: Correspondence GUO Jian-ying, E-mail: guojianying@caas.cn; ZHOU Zhong-shi, Tel: +86-10-62810159, E-mail: zhouzhongshi@caas.cn
  • About author:ZHAO Chen-chen, E-mail: zhaochen06166@163.com; YUE Lei, E-mail: nongda-yuelei@163.com;
  • Supported by:
     This study was supported by the National Natural Science Foundation of China (31322046). 

Abstract:

Ophraella communa (Coleoptera: Chrysomelidae), the ragweed leaf beetle, is a biological control agent of the invasive common ragweed, Ambrosia artemisiifolia (Asterales: Asteraceae).  Adults can survive cold conditions that occur during winter.  The adults mate before entering overwintering.  Understanding the connection between copulation and overwintering will be useful for determining O. communa seasonality.  Determining the relationship between overwintering and copulation required comparison of mated and unmated beetles at mean lethal temperature (LTemp50) exposures for 2 h.  Cold-related physiological indices, including the water ratio, super cooling point (SCP), cryoprotectant levels, and energy reserve levels, were also measured.  Mating treatment decreased the LTemp50 of both sexes by reducing their mean SCP and water ratios.  Although the changes of cryoprotectant levels in mated adults were not precisely consistent in between the genders, they increased greatly in both males and females.  Body sugar may play a role in copulation and may also elevate cold hardiness in O. communa

Key words: Ophraella communa ,  mating status ,  cold tolerance ,  hibernation , super cooling point ,  energy reserves