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1.
JIA-2021-0532 利用STTM技术抑制miR397提高甘薯对SPVD的抗性
LI Chen, LIU Xuan-xuan, ABOUELNASR Hesham, MOHAMED HAMED Arisha, KOU Meng, TANG Wei, YAN Hui, WANG Xin, WANG Xiao-xiao, ZHANG Yun-gang, LIU Ya-ju, GAO Run-fei, MA Meng, LI Qiang
Journal of Integrative Agriculture 2022, 21 (
10
): 2865-2875. DOI:
10.1016/j.jia.2022.07.054
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203
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甘薯(
Ipomoea batatas
(L.) Lam.)作为一种重要的杂粮作物,在世界各地广泛种植,但深受甘薯病毒病(SPVD)的影响。本研究利用短串联靶标模拟物(STTM)成功抑制甘薯miR397的表达,上调其靶基因漆酶(
IbLACs
)的表达,使木质素合成途径的上游基因,包括苯丙氨酸解氨酶(
PAL
)、4-香豆酸辅酶A连接酶(
4CL
)、羟基肉桂酰CoA:莽草酸/奎宁酸羟基肉桂酰转移酶(
HTC
)、咖啡酸
O
-甲基转移酶(
COMT
)、肉桂醇脱氢酶(
CAD
)等基因被反馈调控而广泛上调表达。同时,导致PAL和LAC的酶活性显著增加,促进木质素的合成与积累。木质素在细胞壁中的沉积增加了转基因甘薯植株的物理防御能力,有效减少了烟粉虱对SPVD的传播,保证甘薯的健康生长。本研究为甘薯抗病育种和绿色生产提供了新思路。
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2.
Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China
GAO Rui, LI Shi-fang, LU Mei-guang
Journal of Integrative Agriculture 2016, 15 (
7
): 1667-1671. DOI:
10.1016/S2095-3119(16)61343-6
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Cherry virus A
(CVA) is a member of the genus
Capillovirus
, in the family
Betaflexiviridae
. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai’an, Shandong Province, China using high fidelity PCR enzymes and specific primer pairs for amplifying long fragments in RT-PCR and RACE. The full-length sequences from isolates ChTA11 and ChTA12 are both 7 382 nucleotide (nt) long, excluding the poly(A) tail, encode two open reading frames (ORFs) and have similar genome organization to the two isolates in GenBank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function (DUF1717) is more variable compared with other domains. This is the first report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China.
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3.
Effects of long-term phosphorus fertilization and straw incorporation on phosphorus fractions in subtropical paddy soil
LI Yu-yuan, YANG Rui, GAO Ru, WEI Hong-an, CHEN An-lei, LI Yong
Journal of Integrative Agriculture 2015, 14 (
2
): 365-373. DOI:
10.1016/S2095-3119(13)60684-X
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Study on soil phosphorus (P) fraction is an important aspect in probing the mechanisms of soil P accumulation in farmland and mitigating its losing risk to the environment. We used a sequential extraction method to evaluate the impacts of long-term fertilization and straw incorporation on inorganic, organic, and residual P (Pi, Po, and Pre) fractions in the plow layer (0–20 cm) of acidic paddy soil in southern China. The experiment comprised of six treatments: (i) no fertilizer control (CK); (ii) straw incorporation and green manure (SG); (iii) nitrogen and P fertilizer (NP); (iv) NP+SG; (v) NP+K fertilizer (NPK); and (vi) NPK+SG. The results showed that, compared to the initial total soil P content (TSP, 600 mg kg–1 in 1990), long-term (20 years) combined continuous P fertilizer and SG significantly increased P accumulation (by 13–20%) while single fertilization (39.3 kg P ha–1 yr–1) could maintain soil P status at the most. The average soil P fractions comprised of extractable Pi, Po, and Pre by 51.7, 33.4, and 14.9% in total soil P, respectively. With comparison of no fertilizer addition (CK), long-term single fertilization significantly (P<0.05) increased the accumulation of NaHCO3 –, NaOH–, and HCl– extractable Pi fractions accounting for two- to three-fold, while SG increased the accumulation of NaHCO3 – and NaOH– extractable Pi and Po accounting for 12–60%. Though the mobilization of Pre fractions was not significant (P>0.05), our data indicate that SG may partially substitute for fertilizer P input and minimizing soil P accumulation and subsequent environmental risk in the subtropical paddy soil.
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