Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (7): 1667-1671.DOI: 10.1016/S2095-3119(16)61343-6

• 论文 • 上一篇    

Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China

  

  • 收稿日期:2015-09-02 出版日期:2016-07-06 发布日期:2016-07-06

Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China

GAO Rui, LI Shi-fang, LU Mei-guang   

  1. State Key Laboratory of Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • Received:2015-09-02 Online:2016-07-06 Published:2016-07-06
  • Contact: LU Mei-guang, Tel: +86-10-62815615, E-mail: mglu@ippcaas.cn
  • About author:GAO Rui, E-mail: jiaoxiaoleng@163.com
  • Supported by:

    This work was supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201203076) and the National Natural Science Foundation of China (31471752).

Abstract:     Cherry virus A (CVA) is a member of the genus Capillovirus, in the family Betaflexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai’an, Shandong Province, China using high fidelity PCR enzymes and specific primer pairs for amplifying long fragments in RT-PCR and RACE. The full-length sequences from isolates ChTA11 and ChTA12 are both 7 382 nucleotide (nt) long, excluding the poly(A) tail, encode two open reading frames (ORFs) and have similar genome organization to the two isolates in GenBank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function (DUF1717) is more variable compared with other domains. This is the first report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China.

Key words: Cherry virus A (CVA) ,  genome sequence ,  Capillovirus