Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

doi:10.1016/S2095-3119(14)60899-6

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (3): 469-482.DOI: 10.1016/S2095-3119(14)60899-6

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

WANG Shun-li, Seong Sub Ku, YE Xing-guo, HE Cong-fen, Suk Yoon Kwon, Pil Son Choi

1、Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and
Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、Department of Oriental Pharmaceutical Development, Nambu University, Gwangju 506–824, Korea
3、Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic
Improvement, Beijing 100081, P.R.China
4、School of Sciences, Beijing Technology and Business University, Beijing 100048, P.R.China
5、Plant Systems Engineering Research Center, KRIBB, Daejeon 305–806, Korea

收稿日期:2014-08-22 出版日期:2015-03-01 发布日期:2015-03-12
通讯作者: YE Xing-guo, Tel: +86-10-82109765,Fax: +86-10-82105819, E-mail: yexingguo@caas.cn;Pil Son Choi, E-mail: cps6546@hanmail.net
基金资助:
This research was financially supported by grants from the Biogreen 21 Program, RDA, Korea (PJ00810304), the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences (2014–2015) and the Beijing Municipal Education Commission, China (KM200910011001).

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

WANG Shun-li, Seong Sub Ku, YE Xing-guo, HE Cong-fen, Suk Yoon Kwon, Pil Son Choi

1、Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture/Institute of Vegetables and
Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、Department of Oriental Pharmaceutical Development, Nambu University, Gwangju 506–824, Korea
3、Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic
Improvement, Beijing 100081, P.R.China
4、School of Sciences, Beijing Technology and Business University, Beijing 100048, P.R.China
5、Plant Systems Engineering Research Center, KRIBB, Daejeon 305–806, Korea

Received:2014-08-22 Online:2015-03-01 Published:2015-03-12
Contact: YE Xing-guo, Tel: +86-10-82109765,Fax: +86-10-82105819, E-mail: yexingguo@caas.cn;Pil Son Choi, E-mail: cps6546@hanmail.net
Supported by:
This research was financially supported by grants from the Biogreen 21 Program, RDA, Korea (PJ00810304), the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences (2014–2015) and the Beijing Municipal Education Commission, China (KM200910011001).

摘要/Abstract

摘要： Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

关键词: Cucumis sativus L. , plant regeneration , genetic transformation , positive selection system

Abstract: Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.

Key words: Cucumis sativus L. , plant regeneration , genetic transformation , positive selection system

WANG Shun-li, Seong Sub Ku, YE Xing-guo, HE Cong-fen, Suk Yoon Kwon, Pil Son Choi. Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)[J]. Journal of Integrative Agriculture, 2015, 14(3): 469-482.

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Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

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