Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum

doi:10.1016/S2095-3119(13)60505-5

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (11): 2083-2091.DOI: 10.1016/S2095-3119(13)60505-5

Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum

LIU Yi-peng, LIN Ke-jian, LIU Yang, GUI Fu-rong , WANG Gui-rong

1.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100193, P.R.China
2.College of Plant Protection, Yunnan Agricultural University, Yunnan 650201, P.R.China

收稿日期:2013-01-08 出版日期:2013-11-01 发布日期:2013-12-08
通讯作者: Correspondence WANG Gui-rong, Tel: +86-10-62816247, E-mail: grwang@ippcaas.cn
作者简介:LIU Yi-peng, Mobile: 18600214711, E-mail: liuyipenglyp@126.com
基金资助:
This work was supported by the National 973 Program of China (2012CB114104) and the Beijing Natural Science Foundation, China (6132028).

Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum

LIU Yi-peng, LIN Ke-jian, LIU Yang, GUI Fu-rong , WANG Gui-rong

1.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,Beijing 100193, P.R.China
2.College of Plant Protection, Yunnan Agricultural University, Yunnan 650201, P.R.China

Received:2013-01-08 Online:2013-11-01 Published:2013-12-08
Contact: Correspondence WANG Gui-rong, Tel: +86-10-62816247, E-mail: grwang@ippcaas.cn
About author:LIU Yi-peng, Mobile: 18600214711, E-mail: liuyipenglyp@126.com
Supported by:
This work was supported by the National 973 Program of China (2012CB114104) and the Beijing Natural Science Foundation, China (6132028).

摘要/Abstract

摘要： The nicotinic acetylcholine receptors (nAchRs) are cholinergic receptors that form ligand-gated ion channels by five subunits in insect and vertebrate nervous systems. The insect nAChR is the molecular target of a class of insecticides, neonicotinoids. Here, we identified and cloned 11 candidate nAChR subunit genes in Acyrthosiphon pisum using genome-based bioinformatics combined modern molecular techniques. Most A. pisum nAChRs including α1, α2, α3, α4, α6, α8, and β1 show highly sequence identities with the counterparts of other insects examined. Expression profiles analysis showed that all subunit genes were expressed in adult head. At least two subunits have alternative splicing that obviously increase A. pisum nicotinic receptor diversity. This study will be invaluable for exploring the molecular mechanisms of neonicotinoid-like insecticides in sucking pests, and for ultimately establishing the screening platform of novel insecticides.

关键词: Acyrthosiphon pisum , nicotinic acetylcholine receptor , alternative splicing , expression profile

Abstract: The nicotinic acetylcholine receptors (nAchRs) are cholinergic receptors that form ligand-gated ion channels by five subunits in insect and vertebrate nervous systems. The insect nAChR is the molecular target of a class of insecticides, neonicotinoids. Here, we identified and cloned 11 candidate nAChR subunit genes in Acyrthosiphon pisum using genome-based bioinformatics combined modern molecular techniques. Most A. pisum nAChRs including α1, α2, α3, α4, α6, α8, and β1 show highly sequence identities with the counterparts of other insects examined. Expression profiles analysis showed that all subunit genes were expressed in adult head. At least two subunits have alternative splicing that obviously increase A. pisum nicotinic receptor diversity. This study will be invaluable for exploring the molecular mechanisms of neonicotinoid-like insecticides in sucking pests, and for ultimately establishing the screening platform of novel insecticides.

Key words: Acyrthosiphon pisum , nicotinic acetylcholine receptor , alternative splicing , expression profile

LIU Yi-peng, LIN Ke-jian, LIU Yang, GUI Fu-rong, WANG Gui-rong. Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum[J]. Journal of Integrative Agriculture, 2013, 12(11): 2083-2091.

LIU Yi-peng, LIN Ke-jian, LIU Yang, GUI Fu-rong , WANG Gui-rong. Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum[J]. Journal of Integrative Agriculture, 2013, 12(11): 2083-2091.

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Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum

Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid, Acyrthosiphon pisum

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