Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC)

doi:10.1016/S1671-2927(00)9061

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (8): 1471-1480.DOI: 10.1016/S1671-2927(00)9061

Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC)

ZHAO Xiang-hui, LIU Chan-juan, LI Chao-yun , YAO Jun-hu

College of Animal Science and Technology, Northwest A&F University, Yangling 712100, P.R.China

收稿日期:2012-06-05 出版日期:2013-08-01 发布日期:2013-09-12
通讯作者: Correspondence YAO Jun-hu, Tel: +86-29-87092102, E-mail: yaojunhu2004@sohu.com
作者简介:ZHAO Xiang-hui, Tel: +86-29-87092102, E-mail: zhaoxh001@163.com
基金资助:
This research was supported by the National Key Technologies R&D Program of China (2012BAD12B02), the Program of International S&T Cooperation of China (2010DFB34230), and the Scientific & Technological Innovation Project of Shaanxi, China (2011KTCQ02-02).

Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC)

ZHAO Xiang-hui, LIU Chan-juan, LI Chao-yun , YAO Jun-hu

College of Animal Science and Technology, Northwest A&F University, Yangling 712100, P.R.China

Received:2012-06-05 Online:2013-08-01 Published:2013-09-12
Contact: Correspondence YAO Jun-hu, Tel: +86-29-87092102, E-mail: yaojunhu2004@sohu.com
About author:ZHAO Xiang-hui, Tel: +86-29-87092102, E-mail: zhaoxh001@163.com
Supported by:
This research was supported by the National Key Technologies R&D Program of China (2012BAD12B02), the Program of International S&T Cooperation of China (2010DFB34230), and the Scientific & Technological Innovation Project of Shaanxi, China (2011KTCQ02-02).

摘要/Abstract

摘要： We evaluated the effects of neutral detergent soluble fiber (NDSF) and sucrose supplementation on ruminal fermentation, microbial synthesis, and populations of ruminal cellulolytic bacteria using the rumen simulation technique (RUSITEC). The experiment had a 2×2 factorial design with two dosages of sucrose, low (ca. 0.26 g d-1, low-sucrose) and high (ca. 1.01 g d-1, high-sucrose), and two dosages of supplied NDSF, low (1.95 g d-1, low-NDSF) and high (2.70 g d-1, high-NDSF). Interactions between NDSF and sucrose were detected for xylanase activity from solid fraction and apparent disappearance of neutral detergent fiber (NDF) and hemicellulose, with the lowest values observed for high-NDSF and high-sucrose treatment. Supplemental NDSF appeared to increase the molar proportion of acetate and reduce that of butyrate; however, the effects of supplemental sucrose on VFA profiles depended upon NDSF amount. There was a NDSF×sucrose interaction for the production of methane. High-NDSF fermenters had lower ammonia-N production, greater daily N flow of solidassociated microbial pellets and total microorganisms, and greater microbial synthesis efficiency compared with low- NDSF fermenters. Supplementation with NDSF resulted in an increase in 16S rDNA copies of Ruminococcus flavefaciens and a reduction in copies of Ruminococcus albus. Supplementation with sucrose tended to increase the 16S rDNA copies of R. albus from liquid fraction, but did not affect daily total microbial N flow and cellulolytic bacterium populations from solid fraction. These data indicate that the effects of the interaction between NDSF and sugars on ruminal fermentation and fiber digestion should be taken into account in diet formulation. Ruminal fermentation and metabolism of sugars warrant further investigation.

关键词: neutral detergent soluble fiber , sucrose , ruminal fermentation , Rusitec

Abstract: We evaluated the effects of neutral detergent soluble fiber (NDSF) and sucrose supplementation on ruminal fermentation, microbial synthesis, and populations of ruminal cellulolytic bacteria using the rumen simulation technique (RUSITEC). The experiment had a 2×2 factorial design with two dosages of sucrose, low (ca. 0.26 g d-1, low-sucrose) and high (ca. 1.01 g d-1, high-sucrose), and two dosages of supplied NDSF, low (1.95 g d-1, low-NDSF) and high (2.70 g d-1, high-NDSF). Interactions between NDSF and sucrose were detected for xylanase activity from solid fraction and apparent disappearance of neutral detergent fiber (NDF) and hemicellulose, with the lowest values observed for high-NDSF and high-sucrose treatment. Supplemental NDSF appeared to increase the molar proportion of acetate and reduce that of butyrate; however, the effects of supplemental sucrose on VFA profiles depended upon NDSF amount. There was a NDSF×sucrose interaction for the production of methane. High-NDSF fermenters had lower ammonia-N production, greater daily N flow of solidassociated microbial pellets and total microorganisms, and greater microbial synthesis efficiency compared with low- NDSF fermenters. Supplementation with NDSF resulted in an increase in 16S rDNA copies of Ruminococcus flavefaciens and a reduction in copies of Ruminococcus albus. Supplementation with sucrose tended to increase the 16S rDNA copies of R. albus from liquid fraction, but did not affect daily total microbial N flow and cellulolytic bacterium populations from solid fraction. These data indicate that the effects of the interaction between NDSF and sugars on ruminal fermentation and fiber digestion should be taken into account in diet formulation. Ruminal fermentation and metabolism of sugars warrant further investigation.

Key words: neutral detergent soluble fiber , sucrose , ruminal fermentation , Rusitec

ZHAO Xiang-hui, LIU Chan-juan, LI Chao-yun , YAO Jun-hu. Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC)[J]. Journal of Integrative Agriculture, 2013, 12(8): 1471-1480.

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Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC)

Effects of Neutral Detergent Soluble Fiber and Sucrose Supplementation on Ruminal Fermentation, Microbial Synthesis, and Populations of Ruminal Cellulolytic Bacteria Using the Rumen Simulation Technique (RUSITEC)

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