Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (7): 1251-1257.DOI: 10.1016/S2095-3119(13)60436-0

• 论文 • 上一篇    下一篇

Effect of Temperature, pH and Salt on Fluorescent Quality of Water Extractable Organic Matter in Black Soil

 LI Ming-tang, ZHAO Lan-po , ZHANG Jin-jing   

  1. College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, P.R.China
  • 收稿日期:2012-10-15 出版日期:2013-07-01 发布日期:2013-07-04
  • 通讯作者: Correspondence ZHANG Jin-jing, Tel: +86-431-84532955, E-mail: Zhangjinjing@126.com; ZHAO Lan-po, Tel: +86-431-84532955, E-mail: Zhaolanpo12@163.com
  • 基金资助:

    The study was supported by the National Natural Science Foundation of China (51109089 and 31071862).

Effect of Temperature, pH and Salt on Fluorescent Quality of Water Extractable Organic Matter in Black Soil

 LI Ming-tang, ZHAO Lan-po , ZHANG Jin-jing   

  1. College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, P.R.China
  • Received:2012-10-15 Online:2013-07-01 Published:2013-07-04
  • Contact: Correspondence ZHANG Jin-jing, Tel: +86-431-84532955, E-mail: Zhangjinjing@126.com; ZHAO Lan-po, Tel: +86-431-84532955, E-mail: Zhaolanpo12@163.com
  • Supported by:

    The study was supported by the National Natural Science Foundation of China (51109089 and 31071862).

摘要: Water erosion is the major reason for the loss of soil organic carbon in the Northeast China, which leads to the soil quality deterioration and adjacent water pollution. In this study, the effect of extraction temperature, pH value, and salt on the water extractable organic matter (WEOM) was determined by means of the UV absorbance, fluorescence excitationemission matrix, and derived fluorescence indexes. In general, the carbon content and aromaticity of WEOM increased with the increasing of extraction temperature, with the exception that there was no significant difference in the amount at 0 and 20°C. More fluorophores, especially microbially-derived organic matter were extracted at high temperature. The pH values of extractant, including 5, 7, and 10, showed no effect on the carbon amount of WEOM, whereas the aromaticity and microbially-derived component gradually increased with the increasing of pH values. The fluorescence intensity of humic acid-like fluorophore was stronger in neutral and alkali condition than that in acidic condition. The addition of 10 mmol L-1 CaCl2 significantly decreased the carbon amount of recovered WEOM. Moreover, it significantly decreased the aromaticity of WEOM and the quantity of fulvic acid-like and humic acid-like fluorophores, whereas increased the percentage of tyrosine-like and tryptophan-like fluorophores in the total fluorophores and the amount of microbially-derived organic matter. Generally, 10 mmol L-1 KCl showed the same influence trend, but with low influence degree.

关键词: black soil , water extractable organic matter , soil profile , fluorescence spectroscopy

Abstract: Water erosion is the major reason for the loss of soil organic carbon in the Northeast China, which leads to the soil quality deterioration and adjacent water pollution. In this study, the effect of extraction temperature, pH value, and salt on the water extractable organic matter (WEOM) was determined by means of the UV absorbance, fluorescence excitationemission matrix, and derived fluorescence indexes. In general, the carbon content and aromaticity of WEOM increased with the increasing of extraction temperature, with the exception that there was no significant difference in the amount at 0 and 20°C. More fluorophores, especially microbially-derived organic matter were extracted at high temperature. The pH values of extractant, including 5, 7, and 10, showed no effect on the carbon amount of WEOM, whereas the aromaticity and microbially-derived component gradually increased with the increasing of pH values. The fluorescence intensity of humic acid-like fluorophore was stronger in neutral and alkali condition than that in acidic condition. The addition of 10 mmol L-1 CaCl2 significantly decreased the carbon amount of recovered WEOM. Moreover, it significantly decreased the aromaticity of WEOM and the quantity of fulvic acid-like and humic acid-like fluorophores, whereas increased the percentage of tyrosine-like and tryptophan-like fluorophores in the total fluorophores and the amount of microbially-derived organic matter. Generally, 10 mmol L-1 KCl showed the same influence trend, but with low influence degree.

Key words: black soil , water extractable organic matter , soil profile , fluorescence spectroscopy