Population Improvement of Resistance to Late Blight in Tetraploid Potato: A Case Study in Combination with AFLP Marker Assisted Background Selection

doi:10.1016/S1671-2927(11)60108-9

摘要/Abstract

摘要： Cultivated potato with high level of horizontal resistance against late blight is one of the most important goals of potatobreeding. The recurrent selection has been adopted to increase the level of potato horizontal resistance and a B3C1population without R1-R11 dominant genes has been released by the International Potato Center at the short-day conditionof Peru. The present research was carried out to further improve the resistance and the agronomic traits of B3C1population under long-day condition of Hubei, China, with maximized retention of its genetic diversity. Twenty-sevenindividual clones of B3C1 were used to generate population B3C2 by in-population crossing with the bulk pollens aimingto elevate the frequency of late blight resistance genotypes and to improve the adaptation to local long-day conditions.The late blight resistance and the main agronomic traits including the maturity, the plant characters and the tuber traitswere evaluated for the foreground selection in three years, by which 130 pedigrees were maintained as the basic populationof B3C2 for further selection. A total of 312 polymorphic loci detected by 9 AFLP marker combinations were used tomonitor the genetic diversity of the populations for the background selection. The B3C2 population of 51 clones wasfinally selected, of which the frequency of resistant genotypes increased by 23.8% points and the genetic diversity wasmaintained by about 96% as referred to B3C1. Our results strongly suggested that combination of the foregroundselection for target traits and the background selection for the genetic diversity is an efficient strategy in the recurrentselection of tetraploid potato to improve quantitative traits.

null

关键词:

potato, recurrent selection, late blight, horizontal resistance, genetic diversity

Abstract: Cultivated potato with high level of horizontal resistance against late blight is one of the most important goals of potatobreeding. The recurrent selection has been adopted to increase the level of potato horizontal resistance and a B3C1population without R1-R11 dominant genes has been released by the International Potato Center at the short-day conditionof Peru. The present research was carried out to further improve the resistance and the agronomic traits of B3C1population under long-day condition of Hubei, China, with maximized retention of its genetic diversity. Twenty-sevenindividual clones of B3C1 were used to generate population B3C2 by in-population crossing with the bulk pollens aimingto elevate the frequency of late blight resistance genotypes and to improve the adaptation to local long-day conditions.The late blight resistance and the main agronomic traits including the maturity, the plant characters and the tuber traitswere evaluated for the foreground selection in three years, by which 130 pedigrees were maintained as the basic populationof B3C2 for further selection. A total of 312 polymorphic loci detected by 9 AFLP marker combinations were used tomonitor the genetic diversity of the populations for the background selection. The B3C2 population of 51 clones wasfinally selected, of which the frequency of resistant genotypes increased by 23.8% points and the genetic diversity wasmaintained by about 96% as referred to B3C1. Our results strongly suggested that combination of the foregroundselection for target traits and the background selection for the genetic diversity is an efficient strategy in the recurrentselection of tetraploid potato to improve quantitative traits.

Key words:

potato, recurrent selection, late blight, horizontal resistance, genetic diversity

YAO Chun-guang, SONG Bo-tao, LIU Jun, WU Cheng-jin, CHENG Qun, LI Da-chun and XIE Conghua. Population Improvement of Resistance to Late Blight in Tetraploid Potato: A Case Study in Combination with AFLP Marker Assisted Background Selection[J]. Journal of Integrative Agriculture, 2011, 10(8): 1177-1187.

YAO Chun-guang, SONG Bo-tao, LIU Jun, WU Cheng-jin, CHENG Qun, LI Da-chun and XIE Conghua. null[J]. Journal of Integrative Agriculture, 2011, 10(8): 1177-1187.

参考文献

[1]      An Z W, Xie L L, Cheng L, Zhou Y, Zhang Q, He X G, Huang HS. 2009. A silver staining procedure for nucleic acids inpolyacrylamide gels without fixation and pretreatment.Analytical Biochemistry, 391, 77-79.
[2]      Bradshaw J E, Dale M F B, Mackay G R. 2009. Improving theyield, processing quality and disease and pest resistance ofpotatoes by genotypic recurrent selection. Euphytica, 170,215-227.
[3]      Chen G, Zhu J. 2003. QGA Station 1.0. Software for ClassicalQuantitative Genetics. Institute of Bioinformatics, ZhejiangUniversity, China. [2006-01-24].
[4]      http://ibi.zju.edu.cn/software/qga/index.htmCollins A, Milbourne D, Ramsay L, Meyer R, Chatot-BalandrasC, Oberhagemann P, de Jong W, Gebhardt C, Bonnel E, WaughR. 1999. QTL for field resistance to late blight in potato arestrongly correlated with maturity and vigour. MolecularBreeding, 5, 387-398.
[5]      Cooke D E L, Lees A K. 2004. Markers, old and new, forexamining Phytophthora infestans diversity. Plant Pathology,53, 692-704.
[6]      Dekkers J C M, Hospital F. 2002. The use of molecular geneticsin the improvement of agricultural populations. NatureReviews Genetics, 3, 22-32.
[7]      Dong Y S, Zhuang B C, Zhao L M, Sun H, He M Y. 2001. Thegenetic diversity of annual wild soybeans grown in China.Theoretical and Applied Genetics, 103, 98-103.
[8]      Doyle J J. 1991. DNA protocols for plants. In: Hewitt G M,Johnson A W B, Young J P W, eds., Molecular Techniques inTaxonomy. North Atlantic Treaty Organization, ScientificAffairs Division, No. 57. pp. 283-293.
[9]      Ewing E E, Šimko I, Smart C D, Bonierbale M W, Mizubuti E SG, May G D, Fry W E. 2000. Genetic mapping from fieldtests of qualitative and quantitative resistance to Phytophthorainfestans in a population derived from Solanum tuberosumand Solanum berthaultii. Molecular Breeding, 6, 25-36.
[10]   Estrada-Ramos N, Pérez-Alvarez O, Henfling J, Malamud O.1983. Testing CIP and ICA potato selection for late blightresistance and yield at Rionegro (LA Selva) station inColombia. In: Hooker W J, ed., Research for the Potato inthe Year 2000. International Potato Center, Lima, Peru. pp.78-79.
[11]   Frisch M, Bohn M, Melchinger A E. 1999. Comparison ofselection strategies for marker-assisted backcrossing of a gene.Crop Science, 39, 1295-1301.
[12]   Fry W E, Goodwin S B. 1997. Resurgence of the Irish potatofamine fungus. Bioscience, 47, 363-371.
[13]   Garelik G. 2002. Taking the bite out of potato blight. Science,298, 1702-1704.
[14]   Gautney T L, Haynes F L. 1983. Recurrent selection for heattolerance in diploid potatoes (Solanum tuberosum subsp.phureja and stenotomum). American Journal of PotatoResearch, 60, 537-542.
[15]   Gebhardt C, Valkonen J P T. 2001. Organization of genescontrolling disease resistance in the potato genome. AnnualReview of Phytopathology, 39, 79-102.
[16]   Ghislain M, Zhang D, Fajardo D, Huamán Z, Hijmans R J. 1999.Marker-assisted sampling of the cultivated Andean potatoSolanum phureja collection using RAPD markers. GeneticResources and Crop Evolution, 46, 547-555.
[17]   Ghislain M, Andrade D, Rodríguez F, Hijmans R J, Spooner DM. 2006. Genetic analysis of the cultivated potato Solanumtuberosum L. phureja group using RAPDs and nuclear SSRs.Theoretical and Applied Genetics, 113, 1515-1527.
[18]   Goodwin S B, Sujkowski L S, Fry W E. 1995. Rapid evolution ofpathogenicity within clonal lineages of the potato late blightdisease fungus. Phytopathology, 85, 669-676.
[19]   Hallauer A R. 1985. Compendium of recurrent selection methodsand their application. Critical Reviews in Plant Sciences, 3,1-33.
[20]   Jansky S H, Peloquin S J. 2006. Advantages of wild diploidSolanum species over cultivated diploid relatives in potatobreeding programs. Genetic Resources and Crop Evolution,53, 669-674.
[21]   Landeo J A, Gastelo M, Beltran G, Diaz L. 2000. Quantifyinggenetic variance for horizontal resistance to late blight inpotato breeding population B3C1. In: Scientist and Farmer:Partners in Research for the 21st Century Program Report1999-2000.
[22]   International Potato Center, Lima, Peru. pp. 63-68.
[23]   Landeo J A, Gastelo M, Pinedo H, Flores F. 1995. Breeding forhorizontal resistance to late blight in potato free of R genes.In: Dowley L J, Bannon E, Cooke L R, Keane T, O’SullivanE, eds., Phytophthora infestans 150. Bole Press Ltd., Dublin,Ireland. pp. 268-274.
[24]   Leonards-Schippers C, Gieffers W, Schäfer-Pregl R, Ritter E,Knapp S J, Salamini F, Gebhardt C. 1994. Quantitativeresistance to Phytophthora infestans in potato: a case studyfor QTL mapping in an allogamous plant species. Genetics,137, 67-77.
[25]   Marczewski W, Flis B, Syller J, Schäfer-Pregl R, Gebhardt C.2001. A major quantitative trait locus for resistance to potatoleafroll virus is located in a resistance hotspot on potatochromosome XI and is tightly linked to N-gene-like markers.Molecular Plant-Microbe Interactions, 14, 1420-1425.
[26]   Mihovilovich E, Munive S, Bonierbale M. 2010. Influence ofday-length and isolates of Phytophthora infestans on fieldresistance to late blight of potato. Theoretical and AppliedGenetics, 120, 1265-1278.
[27]   Oberhagemann P, Chatot-Balandras C, Schäfer-Pregl R, WegenerD, Palomino C, Salamini F, Bonnel E, Gebhardt C. 1999. Agenetic analysis of quantitative resistance to late blight inpotato: towards marker-assisted selection. MolecularBreeding, 5, 399-415.
[28]   Pérez W, Salas A, Raymundo R, Huamán Z, Nelson R, BonierbaleM. 2000. Evaluation of wild potato species for resistance tolate blight. In: Scientist and Farmer: Partners in Researchfor the 21st Century Program Report 1999-2000.
[29]   International Potato Center, Lima, Peru. pp. 49-62.
[30]   Rauscher G M, Smart C D, Simko I, Bonierbale M, Mayton H,Greenland A, Fry W E. 2006. Characterization and mappingof RPi-ber, a novel potato late blight resistance gene fromSolanum berthaultii. Theoretical and Applied Genetics, 112,674-687.
[31]   Sanford L L, Ladd T L. 1979. Selection for resistance to potatoleafhopper in potatoes. II. Progress after two cycles ofphenotypic recurrent selection. American Journal PotatoResearch, 56, 541-547.
[32]   Simko I. 2002. Comparative analysis of quantitative trait loci forfoliage resistance to Phytophthora infestans in tuber-bearingSolanum species. American Journal of Potato Research, 79,125-132.
[33]   Smith J S C, Chin E C L, Shu H, Smith O S, Wall S J, Senior M L,Mitchell S E, Kresovich S, Ziegle J. 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zeamays L.): comparisons with data from RFLP and pedigree.Theoretical and Applied Genetics, 95, 163-173.
[34]   Song J Q, Bradeen J M, Naess S K, Raasch J A, Wielgus S M,Haberlach G T, Liu J, Kuang H H, Austin-Phillips S, Buell CR, Helgeson J P, et al. 2003. Gene RB cloned from Solanumbulbocastanum confers broad spectrum resistance to potatolate blight. Proceedings of the National Academy of Sciencesof the USA, 100, 9128-9133.
[35]   Vleeshouwers V G A A, van Dooijeweert W, Keizer L C P,Sijpkes L, Govers F, Colon L T. 1999. A laboratory assayfor Phytophthora infestans resistance in various Solanumspecies reflects the field situation. European Journal of PlantPathology, 150, 241-250.
[36]   Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, HornesM, Friters A, Pot J, Paleman J, Kuiper M, Zabeau M. 1995.AFLP: a new technique for DNA finger printing. NucleicAcids Research, 23, 4407-4414.
[37]   van der Vossen E, Sikkema A, Hekkert B T L, Gros J, Stevens P,Muskens M, Wouters D, Pereira A, Stiekema W, Allefs S.2003. An ancient R gene from the wild potato species Solanumbulbocastanum confers broad-spectrum resistance toPhytophthora infestans in cultivated potato and tomato. ThePlant Journal, 36, 867-882.
[38]   Wang B L, Liu J, Tian Z D, Song B T, Xie C H. 2005. Monitoringthe expression patterns of potato genes associated withquantitative resistance to late blight during Phytophthorainfestans infection using cDNA microarrays. Plant Science,169, 1155-1167.
[39] Young N D, Tanksley S D. 1989. RFLP analysis of the size ofchromosomal segments retained around the Tm-2 locus oftomato during backcross breeding. Theoretical and AppliedGenetics, 77, 353-359.