Proteomic Study on Two Bradyrhizobium japonicum Strains with Different Competitivenesses for Nodulation

doi:10.1016/S1671-2927(11)60096-5

摘要/Abstract

摘要： Competitiveness for nodulation of Bradyrhizobium japonicum strains plays a key role in symbiotic nitrogen fixation. Inorder to reveal the difference in competitiveness, B. japonicum 4534 with high competitiveness and B. japonicum 4222with low competitiveness for nodulation were analyzed by proteomic technique. The results showed that differentialproteins were fewer when two strains were treated with just daidzein. Only 24 and 10 differential proteins were detectedwith an up-regulated rate of 58 and 40% in B. japonicum 4534 and B. japonicum 4222, respectively. However, moredifferential proteins were detected upon treatment with daidzein and mutual extracellular materials simultaneously. Therewere 78 differential proteins detected in B. japonicum 4534 with 43 being up-regulated and 35 being down-regulated.These differential proteins, such as metabolism-related proteins, transporters, transcription-related proteins, translationrelatedproteins, and flagellin, were found to be associated with nodulation process. 25 up-regulated and 22 down-regulatedproteins were detected in B. japonicum 4222. Some of these proteins were not related to nodulation. More differentialproteins associated with nodulation in B. japonicum 4534 may be the reason for its high competitiveness. The results canprovide a guide to the selection and inoculation of effective strains and are significant to biological nitrogen fixation.

关键词:

text-autospace: " align="left">Bradyrhizobium japonicum strains, daidzein, extracellular materials, competitiveness for nodulation, proteomic technique

Abstract: Competitiveness for nodulation of Bradyrhizobium japonicum strains plays a key role in symbiotic nitrogen fixation. Inorder to reveal the difference in competitiveness, B. japonicum 4534 with high competitiveness and B. japonicum 4222with low competitiveness for nodulation were analyzed by proteomic technique. The results showed that differentialproteins were fewer when two strains were treated with just daidzein. Only 24 and 10 differential proteins were detectedwith an up-regulated rate of 58 and 40% in B. japonicum 4534 and B. japonicum 4222, respectively. However, moredifferential proteins were detected upon treatment with daidzein and mutual extracellular materials simultaneously. Therewere 78 differential proteins detected in B. japonicum 4534 with 43 being up-regulated and 35 being down-regulated.These differential proteins, such as metabolism-related proteins, transporters, transcription-related proteins, translationrelatedproteins, and flagellin, were found to be associated with nodulation process. 25 up-regulated and 22 down-regulatedproteins were detected in B. japonicum 4222. Some of these proteins were not related to nodulation. More differentialproteins associated with nodulation in B. japonicum 4534 may be the reason for its high competitiveness. The results canprovide a guide to the selection and inoculation of effective strains and are significant to biological nitrogen fixation.

Key words:

text-autospace: " align="left">Bradyrhizobium japonicum strains, daidzein, extracellular materials, competitiveness for nodulation, proteomic technique

LI Jun, XIAO Wen-li, MA Ming-chao, GUAN Da-wei, JIANG Xin, CAO Feng-ming, SHEN Delong, CHEN Hui-jun , LI Li. Proteomic Study on Two Bradyrhizobium japonicum Strains with Different Competitivenesses for Nodulation[J]. Journal of Integrative Agriculture, 2011, 10(7): 1072-1079.

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